Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   MKF35_RS24325 Genome accession   NZ_CP092629
Coordinates   5302845..5303516 (-) Length   223 a.a.
NCBI ID   WP_003090494.1    Uniprot ID   A0A0H2ZC88
Organism   Pseudomonas aeruginosa strain HU20     
Function   repress competence development (predicted from homology)   
Competence regulation

Genomic Context

Location: 5297845..5308516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKF35_RS24310 - 5298963..5301107 (+) 2145 WP_003113383.1 methyl-accepting chemotaxis protein -
  MKF35_RS24315 - 5301112..5301420 (-) 309 WP_003113384.1 hypothetical protein -
  MKF35_RS24320 carS 5301511..5302848 (-) 1338 WP_003090493.1 two-component system sensor histidine kinase CarS -
  MKF35_RS24325 ciaR 5302845..5303516 (-) 672 WP_003090494.1 two-component system response regulator CarR Regulator
  MKF35_RS24330 - 5303516..5303830 (-) 315 WP_003097704.1 PepSY domain-containing protein -
  MKF35_RS24335 - 5303830..5304138 (-) 309 WP_003090497.1 PepSY domain-containing protein -
  MKF35_RS24340 - 5304353..5305435 (+) 1083 WP_003113385.1 hypothetical protein -
  MKF35_RS24345 - 5305462..5306355 (+) 894 WP_003116712.1 hypothetical protein -
  MKF35_RS24350 - 5306339..5306605 (+) 267 WP_003115326.1 DUF5329 domain-containing protein -
  MKF35_RS24355 - 5306606..5307799 (-) 1194 WP_047106060.1 NnrS family protein -
  MKF35_RS24360 ppyR 5307786..5308043 (-) 258 WP_003090502.1 psl/pyoverdine operon transcriptional regulator PpyR -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24729.20 Da        Isoelectric Point: 5.8445

>NTDB_id=567264 MKF35_RS24325 WP_003090494.1 5302845..5303516(-) (ciaR) [Pseudomonas aeruginosa strain HU20]
MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA
RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR
YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide


Download         Length: 672 bp        

>NTDB_id=567264 MKF35_RS24325 WP_003090494.1 5302845..5303516(-) (ciaR) [Pseudomonas aeruginosa strain HU20]
ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGACGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT
GGACTGGCTGGCCGACGGCCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGATCTGATCATCCTCGACCTCGGCC
TGCCCGGGCGTCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG
CGCGGTTCCTGGGCCGAACGCATCGATGGGCTCAAGGCCGGTGCCGACGACTACCTGACCAAGCCGTTCCATCCGGAAGA
GCTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCCGGGCTGC
GCCTGGACGAGCAGCGCCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC
TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA
TTCCAACGTCATCGAGGTCCACGTCAACCACCTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG
GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(147-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus mutans UA159

37.727

98.655

0.372
  ciaR Streptococcus pneumoniae Rx1

37.273

98.655

0.368
  ciaR Streptococcus pneumoniae D39

37.273

98.655

0.368
  ciaR Streptococcus pneumoniae R6

37.273

98.655

0.368
  ciaR Streptococcus pneumoniae TIGR4

37.273

98.655

0.368
  micA Streptococcus pneumoniae Cp1015

35.683

100

0.363
  vicR Streptococcus mutans UA159

35.526

100

0.363