Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KFF03_RS15315 Genome accession   NZ_CP073745
Coordinates   3370953..3372014 (-) Length   353 a.a.
NCBI ID   WP_255860940.1    Uniprot ID   A0A9J7ALR8
Organism   Bacterioplanoides sp. SCSIO 12839     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3365953..3377014
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KFF03_RS15290 aroK 3366592..3367101 (-) 510 WP_255857782.1 shikimate kinase AroK -
  KFF03_RS15295 (KFF03_15215) pilQ 3367106..3369211 (-) 2106 WP_255857783.1 type IV pilus secretin PilQ Machinery gene
  KFF03_RS15300 (KFF03_15220) - 3369214..3369753 (-) 540 WP_255857784.1 pilus assembly protein PilP -
  KFF03_RS15305 (KFF03_15225) - 3369755..3370402 (-) 648 WP_255857785.1 type 4a pilus biogenesis protein PilO -
  KFF03_RS15310 (KFF03_15230) - 3370399..3370953 (-) 555 WP_255857786.1 PilN domain-containing protein -
  KFF03_RS15315 (KFF03_15235) pilM 3370953..3372014 (-) 1062 WP_255860940.1 pilus assembly protein PilM Machinery gene
  KFF03_RS15320 (KFF03_15240) - 3372249..3374726 (+) 2478 WP_255857787.1 penicillin-binding protein 1A -
  KFF03_RS15325 (KFF03_15245) - 3374844..3376247 (-) 1404 WP_255857788.1 FAD-binding oxidoreductase -

Sequence

Protein


Download         Length: 353 a.a.        Molecular weight: 38463.21 Da        Isoelectric Point: 4.1202

>NTDB_id=560389 KFF03_RS15315 WP_255860940.1 3370953..3372014(-) (pilM) [Bacterioplanoides sp. SCSIO 12839]
MASLFNKKSKALIGVDISSTSVKVLELSHNNGRYQVEAYASEPLPPNAIVEQAISNDEAVGEAIRKAVGRSRTSAKRAAI
AVAGSAVITKTVQMSSKLSDDEMDLQIRAEADQYIPYPLEEVALDWEVQGESDQVPDSVDILLAACRSETVERRKDAVEY
ADLEAGVVDVEAFCTERAFELLQGQLDGDDIETVAVLDIGATMTTLSVLHEGKSIYTREQLFGGRQLTEDIMRRYGLSEE
EATRSKLDGDLPDDYESEVLEPFRKAVVQQVSRSLQFFYSSSQFNDVDFVVLAGGTSAIPLLAENVEEDLGVATLIANPF
MNMTLSQKVNASLLANDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1062 bp        

>NTDB_id=560389 KFF03_RS15315 WP_255860940.1 3370953..3372014(-) (pilM) [Bacterioplanoides sp. SCSIO 12839]
CTGGCCAGCCTGTTTAATAAGAAGAGCAAAGCACTGATCGGGGTGGATATCAGTTCCACATCAGTCAAGGTACTTGAGCT
AAGTCATAATAATGGCCGATATCAGGTCGAAGCTTATGCCTCTGAACCATTACCACCAAATGCAATTGTTGAACAGGCAA
TCAGTAATGATGAGGCTGTTGGTGAGGCTATTCGTAAGGCTGTTGGACGTTCCCGTACATCCGCTAAGCGTGCTGCTATT
GCCGTCGCGGGTTCTGCTGTTATCACTAAAACGGTTCAGATGAGTTCAAAGCTCAGTGATGATGAAATGGATCTGCAGAT
TCGCGCTGAAGCTGATCAATATATTCCTTACCCGCTTGAAGAAGTTGCACTGGACTGGGAAGTTCAGGGTGAGTCTGATC
AGGTTCCTGACTCTGTTGATATCCTTTTAGCGGCTTGTCGTTCTGAAACTGTAGAGCGTCGCAAAGATGCGGTTGAATAC
GCTGATCTCGAAGCGGGCGTGGTTGACGTTGAGGCGTTTTGTACTGAACGTGCCTTTGAGTTATTACAAGGCCAGTTAGA
CGGTGATGATATTGAGACCGTTGCTGTACTTGATATCGGTGCCACTATGACAACCTTGAGTGTCCTGCATGAAGGAAAGT
CGATTTACACGCGAGAGCAGTTGTTTGGCGGTCGTCAGCTGACAGAAGATATTATGCGGCGTTATGGCTTGTCTGAAGAA
GAAGCGACACGTTCCAAGCTCGATGGTGACCTTCCTGATGACTATGAAAGTGAAGTCTTGGAGCCATTTCGCAAAGCGGT
GGTTCAGCAGGTAAGCCGCTCGCTGCAATTTTTCTATTCATCAAGTCAGTTCAATGACGTTGATTTTGTCGTTCTCGCAG
GTGGAACTTCTGCCATTCCTTTGTTAGCTGAAAATGTTGAAGAGGATTTAGGCGTTGCGACTTTAATTGCTAACCCCTTT
ATGAATATGACCTTATCGCAAAAAGTGAATGCCAGCCTTCTGGCTAACGATGCTCCTTCACTGATGATTGCTTGTGGTTT
GGCAATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

54.674

100

0.547
  comM Acinetobacter nosocomialis M2

54.674

100

0.547
  comM Acinetobacter baylyi ADP1

50.142

100

0.501
  pilM Legionella pneumophila strain ERS1305867

43.662

100

0.439