Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   KB879_RS03130 Genome accession   NZ_CP073677
Coordinates   619437..619952 (+) Length   171 a.a.
NCBI ID   WP_211980736.1    Uniprot ID   -
Organism   Cupriavidus sp. KK10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 614437..624952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KB879_RS03105 (KB879_03105) recX 614463..614930 (+) 468 WP_211980735.1 recombination regulator RecX -
  KB879_RS03110 (KB879_03110) - 615243..615938 (+) 696 WP_011614567.1 DUF2889 domain-containing protein -
  KB879_RS03115 (KB879_03115) sucC 616028..617194 (+) 1167 WP_013955623.1 ADP-forming succinate--CoA ligase subunit beta -
  KB879_RS03120 (KB879_03120) sucD 617294..618175 (+) 882 WP_011614568.1 succinate--CoA ligase subunit alpha -
  KB879_RS03125 (KB879_03125) - 618422..619117 (+) 696 WP_013955624.1 TerC family protein -
  KB879_RS03130 (KB879_03130) pilA 619437..619952 (+) 516 WP_211980736.1 pilin Machinery gene
  KB879_RS03135 (KB879_03135) - 620049..621860 (+) 1812 WP_211980737.1 Wzy polymerase domain-containing protein -
  KB879_RS03140 (KB879_03140) - 621862..622368 (-) 507 WP_211980738.1 hypothetical protein -
  KB879_RS03145 (KB879_03145) - 622383..622817 (-) 435 WP_013955628.1 hypothetical protein -
  KB879_RS03150 (KB879_03150) moaC 623046..623525 (-) 480 WP_013955629.1 cyclic pyranopterin monophosphate synthase MoaC -

Sequence

Protein


Download         Length: 171 a.a.        Molecular weight: 17158.79 Da        Isoelectric Point: 10.0092

>NTDB_id=559867 KB879_RS03130 WP_211980736.1 619437..619952(+) (pilA) [Cupriavidus sp. KK10]
MQRVQQLKKLGRRVQKGFTLIELMIVVAIIGILAAIAIPAYQDYLIRSRVTEGLSLAASAKTSVTENAANGIALANGWTA
PSPTQNVTAMAVDATGQITITYSARAGGGTLVLVPSSAAAALAVGTPPAGGSVDWECFSAAKAASTLPTPGPAPATAATL
PGNVSPASCRS

Nucleotide


Download         Length: 516 bp        

>NTDB_id=559867 KB879_RS03130 WP_211980736.1 619437..619952(+) (pilA) [Cupriavidus sp. KK10]
ATGCAACGGGTACAACAACTGAAGAAACTGGGCCGTCGTGTCCAGAAGGGCTTTACGCTGATCGAACTGATGATCGTGGT
GGCGATCATTGGTATTTTGGCGGCGATTGCTATTCCGGCGTATCAGGACTATCTGATTCGTTCTCGGGTTACCGAAGGCC
TGAGCTTGGCCGCTTCAGCCAAGACGTCAGTTACCGAAAATGCAGCGAACGGCATTGCGCTTGCTAACGGTTGGACGGCT
CCATCCCCGACGCAAAACGTAACGGCTATGGCAGTCGACGCTACTGGGCAAATCACGATCACGTATTCTGCTCGCGCCGG
TGGCGGTACGCTGGTCCTGGTGCCCTCGTCTGCCGCCGCCGCTCTGGCAGTCGGCACTCCGCCGGCCGGTGGTTCGGTGG
ATTGGGAGTGCTTCTCGGCGGCCAAGGCTGCGTCGACTCTGCCGACTCCGGGGCCGGCACCTGCCACGGCCGCCACGCTT
CCGGGTAACGTCTCCCCTGCAAGCTGCCGCAGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(47-170)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-37)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

52.874

100

0.538
  pilA2 Legionella pneumophila str. Paris

52.174

94.152

0.491
  pilA2 Legionella pneumophila strain ERS1305867

51.553

94.152

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

36.269

100

0.409
  comP Acinetobacter baylyi ADP1

45

81.871

0.368