Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KCG52_RS07550 Genome accession   NZ_CP073114
Coordinates   1577156..1579288 (+) Length   710 a.a.
NCBI ID   WP_254343584.1    Uniprot ID   -
Organism   Neisseria subflava strain HP0048     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1572156..1584288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCG52_RS07530 (KCG52_07510) pilM 1574187..1575278 (+) 1092 WP_254343583.1 type IV pilus assembly protein PilM -
  KCG52_RS07535 (KCG52_07515) pilN 1575283..1575906 (+) 624 WP_036492821.1 PilN domain-containing protein Machinery gene
  KCG52_RS07540 (KCG52_07520) pilO 1575906..1576577 (+) 672 WP_003748967.1 type 4a pilus biogenesis protein PilO -
  KCG52_RS07545 (KCG52_07525) pilP 1576593..1577135 (+) 543 WP_049347906.1 pilus assembly protein PilP Machinery gene
  KCG52_RS07550 (KCG52_07530) pilQ 1577156..1579288 (+) 2133 WP_254343584.1 type IV pilus secretin PilQ Machinery gene
  KCG52_RS07555 (KCG52_07535) - 1579419..1579931 (+) 513 WP_283254529.1 shikimate kinase -
  KCG52_RS07560 (KCG52_07540) aroB 1579953..1581032 (+) 1080 WP_107848890.1 3-dehydroquinate synthase -
  KCG52_RS07565 (KCG52_07545) - 1581162..1582475 (-) 1314 WP_254343586.1 ATP-binding protein -
  KCG52_RS07570 (KCG52_07550) - 1582505..1583182 (-) 678 WP_254343588.1 response regulator -
  KCG52_RS07575 (KCG52_07555) - 1583328..1583894 (-) 567 WP_049328898.1 NADPH-dependent FMN reductase -

Sequence

Protein


Download         Length: 710 a.a.        Molecular weight: 78043.19 Da        Isoelectric Point: 9.6083

>NTDB_id=558007 KCG52_RS07550 WP_254343584.1 1577156..1579288(+) (pilQ) [Neisseria subflava strain HP0048]
MKTKHMTKLFAGFSVALAVQTAFAGNITDINVSTLPDNQKIIKIRFDKDVTTPHGFVTSTPARIALDFANTNIRLPQPVL
EYADPLLNQITAAQNNDRARVVLGLNKISQYNTEIRDNEVWVFVNESTDQTNAAVVNERAATPSTARATQAYQAVSAANI
DFRKGARNSGIIELSAPGFSGQPDIKQQRDRVVVTLKNHTLPTQAQRSLDVADFNTPVQNVTLKRIGNSTQLIIRNNNAN
WDINTKASSGRFVFEVSPKAANTESGGLNQNANKSFKGRKISLDFQDVEVRTILQILAKESGVNIVASDTVKGTMTLSLK
DVPWDQALDLVMQARNLDMRRQGNIINIAPRDELLAKDKAFLQAEKEIAELGPLYSQTFQLKYKNVEEFRKILRLEEYDN
NNSNTRNTLLSNRGSALIDPATNTLIVTDNRGVIEKFRKLIDELDVPTRQVMVEARIVEAEDTFFRNLGVKFGSGGASGR
TAWGSNWSNAQANYNTNASLNRGDIGYRTWSLDPNVSLPTAAAVNSIALVRAFSSGALGLEISASEEQGKSKTISNPRVL
TQDRKEAKIESGTEIPYQEASSSGATSITFKKAVLGLTVTPNITPDGQIIMTVKINRDTPIDCTVDSLTTKCINTKHLNT
QAMVEDGGTLIVGGIYEEESTNAVNKVPVLGDIPVVGNLFKSRGKRENRRELLIFITPRIMDNVGNNLRY

Nucleotide


Download         Length: 2133 bp        

>NTDB_id=558007 KCG52_RS07550 WP_254343584.1 1577156..1579288(+) (pilQ) [Neisseria subflava strain HP0048]
ATGAAAACCAAGCACATGACAAAATTATTTGCCGGCTTCAGCGTTGCCCTCGCTGTTCAGACGGCCTTTGCAGGCAATAT
TACCGATATCAACGTTTCCACTTTGCCTGACAACCAAAAAATCATCAAAATCCGTTTCGACAAAGACGTCACCACACCTC
ATGGTTTTGTAACATCTACTCCGGCGCGTATTGCATTGGATTTTGCCAACACCAATATCCGTTTGCCCCAGCCTGTTTTG
GAATATGCCGACCCCTTGCTGAATCAAATTACTGCGGCACAAAACAACGACCGCGCACGCGTGGTTTTAGGTTTGAACAA
AATCAGTCAATACAATACTGAAATTCGCGACAACGAAGTTTGGGTATTCGTAAACGAATCTACTGACCAAACCAATGCGG
CCGTTGTAAATGAACGTGCGGCTACACCGTCTACCGCGCGCGCTACTCAGGCTTATCAAGCCGTTTCGGCCGCCAATATC
GATTTCCGCAAAGGCGCACGCAATTCCGGCATTATTGAACTGTCTGCCCCGGGTTTCAGCGGACAGCCGGACATTAAACA
GCAACGCGACCGCGTCGTTGTTACCTTGAAAAACCATACGCTGCCGACACAAGCGCAACGCAGTTTGGATGTGGCTGACT
TCAATACGCCTGTACAAAACGTTACGCTCAAACGTATCGGCAATTCTACCCAGCTCATTATCCGCAACAATAATGCGAAT
TGGGACATTAATACCAAAGCCTCTTCCGGCCGTTTTGTATTTGAAGTATCGCCTAAAGCCGCCAATACCGAATCCGGCGG
CCTGAACCAAAATGCAAACAAATCGTTTAAAGGCCGTAAAATTTCCTTGGACTTCCAAGATGTAGAAGTCCGTACCATTT
TGCAGATTTTGGCGAAAGAGTCCGGCGTGAATATTGTTGCCAGCGATACCGTCAAAGGCACCATGACCCTGTCCTTGAAA
GATGTACCTTGGGATCAGGCTTTGGACTTGGTTATGCAGGCGCGCAATTTGGATATGCGCCGTCAAGGCAACATCATCAA
TATCGCCCCCCGCGATGAATTGTTGGCAAAAGACAAAGCCTTCTTACAGGCGGAAAAAGAGATTGCCGAATTGGGTCCGC
TGTATTCTCAAACCTTCCAGTTGAAATACAAAAACGTGGAAGAATTCCGCAAAATTTTGCGTTTGGAAGAATACGACAAC
AATAATTCCAATACTCGCAACACTCTGTTGAGCAACCGAGGCAGCGCCTTAATCGATCCGGCCACCAATACGCTGATCGT
AACCGACAACCGCGGCGTGATTGAGAAATTCCGCAAACTGATTGATGAATTGGACGTTCCGACCCGTCAAGTGATGGTTG
AAGCGCGCATTGTCGAAGCAGAAGATACCTTCTTCCGCAATTTGGGCGTTAAATTCGGTTCCGGTGGTGCGAGCGGCCGT
ACGGCATGGGGCAGCAACTGGAGCAATGCGCAAGCCAACTACAACACCAATGCCTCGTTAAACCGTGGCGACATAGGCTA
CCGCACATGGTCTTTGGATCCGAACGTCAGCCTGCCGACCGCAGCCGCAGTCAACAGCATTGCGCTGGTGCGTGCGTTCT
CGTCCGGCGCATTGGGTTTGGAAATCAGCGCGTCTGAAGAACAAGGTAAGAGCAAAACCATTTCCAATCCACGCGTGCTG
ACACAAGACCGTAAAGAAGCCAAAATTGAATCCGGTACGGAAATCCCTTACCAAGAAGCATCTTCCAGCGGTGCGACTTC
GATTACCTTCAAGAAAGCCGTTTTGGGCTTGACCGTAACGCCGAACATTACGCCTGACGGCCAAATCATCATGACTGTGA
AGATTAATCGCGATACCCCGATCGACTGTACCGTAGATTCTCTGACAACCAAGTGTATCAACACCAAACACTTGAATACC
CAAGCCATGGTTGAAGATGGCGGTACGCTGATTGTCGGCGGTATTTATGAGGAAGAAAGCACCAATGCAGTGAACAAAGT
CCCCGTTTTGGGCGATATTCCCGTTGTCGGCAATTTGTTCAAATCACGCGGCAAGCGTGAAAACCGCCGTGAGCTGTTGA
TTTTCATCACGCCGCGCATTATGGATAATGTGGGTAACAATCTGCGTTATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-117)

Secretin and TonB N terminus short domain (STN)

(303-350)

Bacterial type II and III secretion system protein (Secretin)

(545-702)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-450)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Neisseria gonorrhoeae strain FA1090

64.586

100

0.67
  pilQ Neisseria meningitidis MC58

60.203

100

0.669
  pilQ Neisseria meningitidis M1080

60.819

100

0.669