Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   KBU60_RS01645 Genome accession   NZ_CP073068
Coordinates   348047..348640 (+) Length   197 a.a.
NCBI ID   WP_005480482.1    Uniprot ID   Q87L64
Organism   Vibrio parahaemolyticus strain FB-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 343047..353640
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBU60_RS01630 (KBU60_01630) - 343763..346333 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  KBU60_RS01635 (KBU60_01635) pilM 346476..347492 (+) 1017 WP_005480475.1 type IV pilus assembly protein PilM Machinery gene
  KBU60_RS01640 (KBU60_01640) pilN 347476..348054 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  KBU60_RS01645 (KBU60_01645) pilO 348047..348640 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  KBU60_RS01650 (KBU60_01650) pilP 348630..349145 (+) 516 WP_140279392.1 pilus assembly protein PilP Machinery gene
  KBU60_RS01655 (KBU60_01655) pilQ 349177..350925 (+) 1749 WP_212569295.1 type IV pilus secretin PilQ family protein Machinery gene
  KBU60_RS01660 (KBU60_01660) aroK 351114..351632 (+) 519 WP_005381319.1 shikimate kinase AroK -
  KBU60_RS01665 (KBU60_01665) aroB 351664..352764 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23091.63 Da        Isoelectric Point: 4.8225

>NTDB_id=557637 KBU60_RS01645 WP_005480482.1 348047..348640(+) (pilO) [Vibrio parahaemolyticus strain FB-11]
MVNWQDLEMDEIAEWPLLPQLVVICLLVLLIQGFGYWFYLKPEEARLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSENFLYRLPLNIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEEKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=557637 KBU60_RS01645 WP_005480482.1 348047..348640(+) (pilO) [Vibrio parahaemolyticus strain FB-11]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATTGCTGAATGGCCATTACTGCCTCAGCTTGTAGTGATCTGCCTGCT
TGTTCTTCTTATCCAAGGGTTTGGTTATTGGTTTTACTTAAAGCCAGAAGAGGCTCGCTTGAACAATTTGATTCAAGAGG
AGCAAACATTAAAAGTGGCATTGCGCATAAAAGCCAACAAGGTGGCTGCATTGCCTCAGTTGCAATCTCAACTGGACGAG
CTCACTACGCGTTATGAGTTGCTGTTGCAACAACTACCGGCACAAAAAGAGTTGGCCACAATGTTGGCGTCGGTAAACGA
ACTCGGTATCGACAACAAGTTAACGTTTACTCGAATCGATTGGGGAGAAAAGCAATCTGAAAACTTCCTGTATCGATTGC
CACTAAATATCGAGCTAACAGGTGATTTCCACGATATCGGTCGTTTTTCACAGGCCATTGCCACATTGCCACGAATCATC
ACCTTTAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTTTGCACTTCCGAGTGAGAGCAAACACTTATCA
GTTCAAGCCTGAGGAAAAGAAAGATGAGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q87L64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

94.211

96.447

0.909
  pilO Vibrio cholerae strain A1552

72.589

100

0.726