Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   KBU60_RS01640 Genome accession   NZ_CP073068
Coordinates   347476..348054 (+) Length   192 a.a.
NCBI ID   WP_015297396.1    Uniprot ID   A0A243VIJ8
Organism   Vibrio parahaemolyticus strain FB-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 342476..353054
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBU60_RS01625 (KBU60_01625) oxyR 342799..343707 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  KBU60_RS01630 (KBU60_01630) - 343763..346333 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  KBU60_RS01635 (KBU60_01635) pilM 346476..347492 (+) 1017 WP_005480475.1 type IV pilus assembly protein PilM Machinery gene
  KBU60_RS01640 (KBU60_01640) pilN 347476..348054 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  KBU60_RS01645 (KBU60_01645) pilO 348047..348640 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  KBU60_RS01650 (KBU60_01650) pilP 348630..349145 (+) 516 WP_140279392.1 pilus assembly protein PilP Machinery gene
  KBU60_RS01655 (KBU60_01655) pilQ 349177..350925 (+) 1749 WP_212569295.1 type IV pilus secretin PilQ family protein Machinery gene
  KBU60_RS01660 (KBU60_01660) aroK 351114..351632 (+) 519 WP_005381319.1 shikimate kinase AroK -
  KBU60_RS01665 (KBU60_01665) aroB 351664..352764 (+) 1101 WP_005480443.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 192 a.a.        Molecular weight: 22438.95 Da        Isoelectric Point: 6.9944

>NTDB_id=557636 KBU60_RS01640 WP_015297396.1 347476..348054(+) (pilN) [Vibrio parahaemolyticus strain FB-11]
MLYRINLLPWRENQREEHRRRFVGLVVLGVMVALGIQWGVGKYYEYQQDLQQERLDYLTTYISDLDKRIEAMKIAELEHS
KILERLKVVEALQNGRNKTTDFMNLMPAVIPEGVYVDKIKMNDLEIEISGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKARFGKEFQTFKVSFMFNTVEEKKEGKQHG

Nucleotide


Download         Length: 579 bp        

>NTDB_id=557636 KBU60_RS01640 WP_015297396.1 347476..348054(+) (pilN) [Vibrio parahaemolyticus strain FB-11]
GTGCTTTATCGCATTAACTTATTGCCTTGGCGCGAAAATCAAAGGGAAGAGCATCGTCGTCGATTTGTCGGTTTAGTGGT
ATTGGGCGTCATGGTGGCGTTGGGGATTCAGTGGGGCGTTGGCAAATATTATGAGTATCAACAAGATCTTCAACAAGAGC
GACTCGACTATCTAACAACGTACATTTCCGATCTAGATAAGCGGATAGAAGCGATGAAGATCGCGGAGCTTGAACACAGC
AAAATCTTGGAACGCTTGAAAGTTGTGGAAGCGTTGCAAAATGGTCGGAATAAAACCACTGATTTTATGAACCTCATGCC
TGCTGTTATTCCAGAAGGCGTGTATGTCGACAAGATCAAAATGAACGATCTAGAAATTGAAATCTCAGGTATTTCAGACA
GTACACCACGGCTAGCAACCATGCTCGATAACATGGAACGTTCTGCCAAGCTACTGGACGTTGAAATGCACTCTATCGTG
CACGGAAAAGCCCGTTTTGGTAAAGAGTTTCAGACCTTTAAAGTGTCCTTCATGTTTAACACGGTAGAAGAGAAGAAAGA
GGGAAAACAACATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A243VIJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

83.333

100

0.833
  pilN Vibrio cholerae strain A1552

62.983

94.271

0.594