Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KBU60_RS01635 Genome accession   NZ_CP073068
Coordinates   346476..347492 (+) Length   338 a.a.
NCBI ID   WP_005480475.1    Uniprot ID   A0A0L7V0I6
Organism   Vibrio parahaemolyticus strain FB-11     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 341476..352492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KBU60_RS01620 (KBU60_01620) - 341900..342628 (-) 729 WP_140279390.1 redoxin family protein -
  KBU60_RS01625 (KBU60_01625) oxyR 342799..343707 (+) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  KBU60_RS01630 (KBU60_01630) - 343763..346333 (-) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  KBU60_RS01635 (KBU60_01635) pilM 346476..347492 (+) 1017 WP_005480475.1 type IV pilus assembly protein PilM Machinery gene
  KBU60_RS01640 (KBU60_01640) pilN 347476..348054 (+) 579 WP_015297396.1 PilN domain-containing protein Machinery gene
  KBU60_RS01645 (KBU60_01645) pilO 348047..348640 (+) 594 WP_005480482.1 type 4a pilus biogenesis protein PilO Machinery gene
  KBU60_RS01650 (KBU60_01650) pilP 348630..349145 (+) 516 WP_140279392.1 pilus assembly protein PilP Machinery gene
  KBU60_RS01655 (KBU60_01655) pilQ 349177..350925 (+) 1749 WP_212569295.1 type IV pilus secretin PilQ family protein Machinery gene
  KBU60_RS01660 (KBU60_01660) aroK 351114..351632 (+) 519 WP_005381319.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37776.31 Da        Isoelectric Point: 6.8323

>NTDB_id=557635 KBU60_RS01635 WP_005480475.1 346476..347492(+) (pilM) [Vibrio parahaemolyticus strain FB-11]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPEKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=557635 KBU60_RS01635 WP_005480475.1 346476..347492(+) (pilM) [Vibrio parahaemolyticus strain FB-11]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTACTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCAAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTTGTACGCCTTCGTGCTGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGCAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGAAAAAAACAAATACTG
TTTGTTAGACATCGGCTCGCTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACGCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAACGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTGTCGCATCAGTTGGCATTGGAGTGTGAGTTGTTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0L7V0I6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.911

100

0.849
  pilM Vibrio cholerae strain A1552

48.368

99.704

0.482