Detailed information NTG0556912

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KBP49_RS05665
• Genome accession: NZ_CP072933
• Coordinates: 1312712..1313842 (+)
• Length: 376 a.a.
• NCBI ID: WP_071869741.1
• Uniprot ID: -
• Organism: Xylella fastidiosa subsp. multiplex strain AlmaEM3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1307712..1318842

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KBP49_RS05645 (KBP49_05645)	-	1307991..1308815 (+)	825	WP_004084943.1	glycosyltransferase family 2 protein	-
KBP49_RS05650 (KBP49_05650)	-	1309445..1310272 (-)	828	WP_004084945.1	glycosyltransferase family 2 protein	-
KBP49_RS05655 (KBP49_05655)	-	1310657..1310869 (-)	213	WP_071869740.1	hypothetical protein	-
KBP49_RS05660 (KBP49_05660)	pilT	1311512..1312546 (+)	1035	WP_004084948.1	type IV pilus twitching motility protein PilT	Machinery gene
KBP49_RS05665 (KBP49_05665)	pilU	1312712..1313842 (+)	1131	WP_071869741.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KBP49_RS05670 (KBP49_05670)	-	1314535..1315503 (-)	969	WP_071869748.1	YafY family protein	-
KBP49_RS05675 (KBP49_05675)	-	1316286..1317095 (+)	810	WP_021358437.1	DUF481 domain-containing protein	-
KBP49_RS05680 (KBP49_05680)	hemC	1317149..1318066 (-)	918	WP_004084955.1	hydroxymethylbilane synthase	-
KBP49_RS05685 (KBP49_05685)	-	1318074..1318832 (-)	759	WP_027700753.1	LytTR family DNA-binding domain-containing protein	-

Sequence

Protein

• Length: 376 a.a.
• Molecular weight: 42506.76 Da
• Isoelectric Point: 6.5563

>NTDB_id=556912 KBP49_RS05665 WP_071869741.1 1312712..1313842(+) (pilU) [Xylella fastidiosa subsp. multiplex strain AlmaEM3]
MTTIDFTSLLKLMAHQKASDLFITCGMPPSMKIHGNITPITQVPLTSEQSRDIVFNIMSEAQREEFEKVHECNFAIDITS
IGRFRVNAFYQRNQIGMVLRRIEMSIPTFEKLKLPQVAKELVMEKRGIIIIVGATGSGKSTSMASMINHRNHNSTGHIVT
IEDPIEFIHKHEKCIITQREIGTDTDSWENALKNTLRQAPDVIVIGEVRTQETMDHAISFSETGHLVMCTLHANNANQAI
DRIINFFPEERRSQLLMDLSLNLKGIIAQQLIPSADGKERCLAAEVLRDSRLVQDYIRVGEIHKLKEVMQNHNDLGMTTF
DQSLVELYHNGAINYKEALRHADSQNEVRLRIKLSDGKDAKTLSQGFDGIEIAEMS

Nucleotide

• Length: 1131 bp

>NTDB_id=556912 KBP49_RS05665 WP_071869741.1 1312712..1313842(+) (pilU) [Xylella fastidiosa subsp. multiplex strain AlmaEM3]
ATGACTACCATCGATTTCACATCCTTACTTAAGCTGATGGCGCACCAAAAAGCGTCGGATTTATTTATTACCTGTGGAAT
GCCGCCTTCAATGAAGATTCACGGCAATATCACTCCAATCACACAAGTACCGCTGACTTCAGAGCAAAGCCGTGACATAG
TCTTCAATATCATGAGTGAAGCGCAGCGCGAGGAATTCGAGAAGGTCCACGAATGCAACTTCGCAATTGACATCACCAGC
ATTGGTCGCTTCCGTGTCAACGCTTTCTATCAGCGTAACCAAATTGGTATGGTGTTACGCAGAATCGAGATGTCTATTCC
CACCTTCGAAAAACTGAAGCTCCCGCAGGTCGCTAAGGAGCTCGTCATGGAGAAGCGCGGCATCATCATCATCGTTGGTG
CTACGGGTAGTGGTAAATCGACCTCAATGGCATCAATGATCAACCACCGTAATCACAACTCAACAGGACATATCGTCACT
ATCGAAGATCCCATTGAGTTTATTCACAAGCACGAGAAATGCATCATCACCCAACGCGAAATAGGTACCGATACCGACAG
TTGGGAAAATGCCTTGAAAAATACCCTACGCCAAGCCCCAGATGTGATTGTGATTGGTGAAGTGCGTACTCAAGAGACAA
TGGATCATGCCATCTCGTTCTCCGAAACCGGCCATCTGGTCATGTGTACCCTACATGCCAACAACGCCAATCAAGCAATA
GACCGCATTATTAACTTCTTCCCGGAAGAGCGGCGCAGCCAATTACTGATGGATCTGTCGCTGAATCTAAAAGGCATCAT
CGCTCAACAATTGATCCCATCTGCGGATGGTAAAGAGCGCTGTTTAGCAGCGGAAGTTTTGCGCGACAGTCGATTAGTAC
AGGACTACATACGCGTAGGCGAGATCCATAAGCTCAAAGAAGTCATGCAAAACCACAATGATCTTGGAATGACGACATTC
GATCAAAGTCTAGTCGAACTCTATCATAACGGAGCCATTAACTATAAAGAGGCACTACGCCATGCTGATTCGCAGAACGA
GGTACGCCTGCGTATTAAACTCTCCGATGGGAAAGACGCTAAAACTCTGAGTCAAGGATTCGATGGAATAGAAATTGCAG
AAATGTCATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-273)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	61.58	97.606	0.601
pilU	Acinetobacter baylyi ADP1	60.169	94.149	0.566
pilU	Vibrio cholerae strain A1552	50.843	94.681	0.481
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.484	96.809	0.402