Detailed information NTG0555517

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: LL229_RS11960
• Genome accession: NZ_CP090823
• Coordinates: 2292188..2292634 (-)
• Length: 148 a.a.
• NCBI ID: WP_031296844.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain 229
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2287188..2297634

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LL229_RS11925 (LL229_2268)	-	2287305..2287682 (-)	378	WP_003129983.1	pyridoxamine 5'-phosphate oxidase family protein	-
LL229_RS11930 (LL229_2269)	-	2287887..2288753 (+)	867	WP_010906309.1	RluA family pseudouridine synthase	-
LL229_RS11935 (LL229_2270)	-	2288792..2289601 (-)	810	WP_010906310.1	metal ABC transporter permease	-
LL229_RS11940 (LL229_2271)	-	2289594..2290331 (-)	738	WP_010906311.1	metal ABC transporter ATP-binding protein	-
LL229_RS11945 (LL229_2272)	-	2290508..2291350 (-)	843	WP_010906312.1	metal ABC transporter substrate-binding protein	-
LL229_RS11950 (LL229_2273)	-	2291347..2291784 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
LL229_RS11955 (LL229_2274)	comGG	2291865..2292149 (-)	285	WP_010906314.1	competence type IV pilus minor pilin ComGG	Machinery gene
LL229_RS11960 (LL229_2275)	comGF	2292188..2292634 (-)	447	WP_031296844.1	competence type IV pilus minor pilin ComGF	Machinery gene
LL229_RS11965 (LL229_2276)	comGE	2292597..2292893 (-)	297	WP_010906316.1	competence type IV pilus minor pilin ComGE	Machinery gene
LL229_RS11970 (LL229_2277)	comGD	2292865..2293281 (-)	417	WP_021216554.1	competence type IV pilus minor pilin ComGD	Machinery gene
LL229_RS11975 (LL229_2278)	comGC	2293256..2293525 (-)	270	WP_023349160.1	competence type IV pilus major pilin ComGC	Machinery gene
LL229_RS11980 (LL229_2279)	-	2293686..2294423 (-)	738	WP_081172231.1	hypothetical protein	-
LL229_RS11985 (LL229_2281)	-	2294627..2295406 (-)	780	WP_081172233.1	peptidoglycan amidohydrolase family protein	-
LL229_RS11990 (LL229_2282)	-	2295406..2295693 (-)	288	WP_010905390.1	phage holin	-
LL229_RS11995 (LL229_03420)	-	2295680..2296006 (-)	327	WP_010905389.1	hypothetical protein	-

Sequence

Protein

• Length: 148 a.a.
• Molecular weight: 17083.85 Da
• Isoelectric Point: 8.6780

>NTDB_id=555517 LL229_RS11960 WP_031296844.1 2292188..2292634(-) (comGF) [Lactococcus lactis subsp. lactis strain 229]
MERKLCDLNFKVKAFTLLECLVALLAISGSALVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQNFL
YVTKDKKLRFGFMSDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 447 bp

>NTDB_id=555517 LL229_RS11960 WP_031296844.1 2292188..2292634(-) (comGF) [Lactococcus lactis subsp. lactis strain 229]
ATGGAAAGGAAATTATGCGACTTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAAT
TTCTGGTTCAGCCCTTGTCATCTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAA
AAGATTGGCAAATTTTCTGTCAGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAACAGAATTTTCTG
TATGTAACTAAAGATAAAAAACTAAGGTTTGGTTTTATGAGTGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTA
TCAGCCAATGCTTTATGATATAAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATC
AAGGAGGTGAAAGAACATTTATTTATCAATTTCCAGAAGATACGTAA

Domains

Predicted by InterProScan.

• Putative Competence protein ComGF (ComGF) (48-137)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	75.54	93.919	0.709
comGF	Streptococcus mutans UA140	47.518	95.27	0.453
comGF	Streptococcus mutans UA159	46.809	95.27	0.446
comGF/cglF	Streptococcus mitis SK321	43.066	92.568	0.399
comGF/cglF	Streptococcus pneumoniae Rx1	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae D39	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae R6	40.426	95.27	0.385
comGF/cglF	Streptococcus pneumoniae TIGR4	40.426	95.27	0.385
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	92.568	0.385