Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J5226_RS09720 Genome accession   NZ_CP072597
Coordinates   2251960..2253687 (-) Length   575 a.a.
NCBI ID   WP_215839713.1    Uniprot ID   -
Organism   Lysobacter sp. K5869     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2246960..2258687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J5226_RS09700 (J5226_09700) - 2247677..2248744 (+) 1068 WP_215839709.1 Nudix family hydrolase -
  J5226_RS09705 (J5226_09705) coaE 2249162..2249776 (-) 615 WP_215839710.1 dephospho-CoA kinase -
  J5226_RS09710 (J5226_09710) - 2249781..2250668 (-) 888 WP_215839711.1 A24 family peptidase -
  J5226_RS09715 (J5226_09715) pilC 2250688..2251956 (-) 1269 WP_215839712.1 type II secretion system F family protein Machinery gene
  J5226_RS09720 (J5226_09720) pilB 2251960..2253687 (-) 1728 WP_215839713.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J5226_RS09725 (J5226_09725) - 2253822..2254676 (+) 855 WP_215839714.1 glycosyltransferase family 2 protein -
  J5226_RS09730 (J5226_09730) - 2254681..2255763 (+) 1083 WP_215839715.1 UDP-N-acetylglucosamine 2-epimerase -
  J5226_RS09735 (J5226_09735) - 2255846..2256769 (+) 924 WP_215839716.1 hypothetical protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62439.65 Da        Isoelectric Point: 5.9236

>NTDB_id=554460 J5226_RS09720 WP_215839713.1 2251960..2253687(-) (pilB) [Lysobacter sp. K5869]
MSTATANLVGITGIARRLVMDGALDEVKAREAMAAASAERKPLASYLAENRLATSAQLAAANSIEFGVPLFDASAMDPGQ
SASRLISEELVRKHNALPLFKRGNKLFIGLADPTNSRALDEIKFQTNLVIEAILVDEDLIKSTLDRWMDTNDALGDTVGD
GEGLDTLEVGKDDEGLGAGGDSGIDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETEYRVRLRIDGLLKQVAKVPNK
LHSRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDDQRDLF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDDQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEVAAGITLYEPVGCPDCTEGYKGRTGIYQVMPMTEEIQAIVLEGGNAMQIAAAATKSGVRDLRRSALVKVL
NGVTSLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=554460 J5226_RS09720 WP_215839713.1 2251960..2253687(-) (pilB) [Lysobacter sp. K5869]
ATGTCCACCGCTACTGCCAATCTGGTGGGAATCACCGGCATCGCCCGGCGTTTGGTCATGGACGGCGCCCTGGACGAGGT
CAAAGCCCGCGAAGCCATGGCCGCGGCCAGCGCCGAGCGCAAGCCGCTGGCCTCATACCTTGCTGAGAATCGGCTGGCGA
CCTCGGCCCAGCTGGCCGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACGCCAGCGCGATGGACCCGGGCCAG
TCGGCCTCGCGCCTGATCAGCGAGGAGCTGGTCCGCAAGCACAACGCCCTGCCGTTGTTTAAGCGCGGCAACAAGCTTTT
CATCGGTCTGGCCGACCCGACCAACAGCCGCGCGCTGGACGAGATCAAGTTCCAGACCAACCTCGTGATCGAGGCGATCC
TGGTCGACGAGGACCTGATCAAGTCGACCCTCGACCGCTGGATGGACACCAACGACGCCCTCGGCGACACGGTCGGCGAC
GGCGAGGGCCTGGACACCCTGGAAGTCGGCAAGGACGACGAGGGCCTGGGCGCCGGCGGCGACTCGGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAAACCGAATACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCGAACAAG
CTGCACTCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGAGCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCTCGGCCGCGCGGCTGGGCATCGAGAAGCTCGGCTACGAGGACGATCAGCGCGATCTGTTC
GTCAACGCGGTGGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGACCAGCGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCAGGCATCA
ACCAGGTGCAGATGAACGTCAAACGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGCCAAGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAGACCGCCGAAATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACGCTGCACACCAACGACGCTCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCCT
CGGTGACCTTGGTGATCGCCCAGCGTCTGGCGCGGCGCCTGCACGACTGCAAGCGCGAGGTTCATCTGCCCGAACACGCG
CTGCTGGCCGAAGGTTTCACCCACGAAGAAGTCGCCGCCGGCATCACCCTCTACGAACCGGTCGGCTGCCCCGACTGCAC
CGAGGGGTATAAGGGCCGTACCGGCATCTACCAGGTCATGCCGATGACCGAAGAAATCCAGGCGATCGTGCTCGAAGGCG
GCAATGCGATGCAGATCGCCGCCGCCGCCACGAAGTCGGGCGTGCGCGACCTGCGCCGCTCGGCGCTGGTCAAGGTGCTC
AACGGCGTCACCAGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(47-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.85

98.609

0.541
  pilB Acinetobacter baumannii D1279779

54.674

98.609

0.539
  pilB Legionella pneumophila strain ERS1305867

51.049

99.478

0.508
  pilF Neisseria gonorrhoeae MS11

46.235

99.304

0.459
  pilB Vibrio cholerae strain A1552

50.778

89.391

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

49.046

91.13

0.447
  pilB Vibrio campbellii strain DS40M4

48.855

91.13

0.445