Detailed information NTG0554245

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: J6600_RS12235
• Genome accession: NZ_CP072563
• Coordinates: 2550570..2551184 (-)
• Length: 204 a.a.
• NCBI ID: WP_121984384.1
• Uniprot ID: -
• Organism: Bacillus sp. LJBV19
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2545570..2556184

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
J6600_RS12205 (J6600_12205)	rpsT	2545873..2546139 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
J6600_RS12210 (J6600_12210)	holA	2546156..2547197 (-)	1042	Protein_2377	DNA polymerase III subunit delta	-
J6600_RS12215 (J6600_12215)	-	2547237..2547388 (-)	152	Protein_2378	hypothetical protein	-
J6600_RS12220 (J6600_12220)	-	2547429..2547563 (+)	135	WP_003152870.1	YqzM family protein	-
J6600_RS12225 (J6600_12225)	comEC	2547582..2549933 (-)	2352	WP_209387446.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
J6600_RS12230 (J6600_12230)	-	2549934..2550503 (-)	570	WP_003152868.1	ComE operon protein 2	-
J6600_RS12235 (J6600_12235)	comEA	2550570..2551184 (-)	615	WP_121984384.1	helix-hairpin-helix domain-containing protein	Machinery gene
J6600_RS12240 (J6600_12240)	comER	2551243..2552064 (+)	822	WP_012118027.1	late competence protein ComER	-
J6600_RS12245 (J6600_12245)	-	2552133..2552870 (-)	738	WP_015417856.1	class I SAM-dependent methyltransferase	-
J6600_RS12250 (J6600_12250)	rsfS	2552867..2553223 (-)	357	WP_007408260.1	ribosome silencing factor	-
J6600_RS12255 (J6600_12255)	yqeK	2553241..2553801 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
J6600_RS12260 (J6600_12260)	-	2553791..2554360 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
J6600_RS12265 (J6600_12265)	yhbY	2554371..2554661 (-)	291	WP_209387447.1	ribosome assembly RNA-binding protein YhbY	-
J6600_RS12270 (J6600_12270)	aroE	2554655..2555497 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22166.25 Da
• Isoelectric Point: 5.2093

>NTDB_id=554245 J6600_RS12235 WP_121984384.1 2550570..2551184(-) (comEA) [Bacillus sp. LJBV19]
MTKAMMTEPLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=554245 J6600_RS12235 WP_121984384.1 2550570..2551184(-) (comEA) [Bacillus sp. LJBV19]
ATGACGAAAGCGATGATGACGGAGCCGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACTCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGAACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae Rx1	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae D39	36.019	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae R6	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368