Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LVO07_RS19490 Genome accession   NZ_CP089930
Coordinates   4120927..4122420 (+) Length   497 a.a.
NCBI ID   WP_233073963.1    Uniprot ID   -
Organism   Escherichia coli strain E69     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4115927..4127420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVO07_RS19470 (LVO07_19470) pilD 4116597..4117379 (+) 783 WP_225762607.1 A24 family peptidase Machinery gene
  LVO07_RS19475 (LVO07_19475) gspS2 4117437..4117841 (+) 405 WP_225762608.1 type II secretion system pilot lipoprotein GspS-beta -
  LVO07_RS19480 (LVO07_19480) gspC 4117995..4118831 (+) 837 WP_300980031.1 type II secretion system protein GspC -
  LVO07_RS19485 (LVO07_19485) gspD 4118867..4120924 (+) 2058 WP_024165166.1 type II secretion system secretin GspD -
  LVO07_RS19490 (LVO07_19490) pilB/pilB1 4120927..4122420 (+) 1494 WP_233073963.1 type II secretion system ATPase GspE Machinery gene
  LVO07_RS19495 (LVO07_19495) gspF 4122420..4123655 (+) 1236 WP_225762610.1 type II secretion system inner membrane protein GspF -
  LVO07_RS19500 (LVO07_19500) gspG 4123672..4124130 (+) 459 WP_000286808.1 type II secretion system major pseudopilin GspG -
  LVO07_RS19505 (LVO07_19505) gspH 4124131..4124685 (+) 555 WP_000946124.1 type II secretion system minor pseudopilin GspH -
  LVO07_RS19510 (LVO07_19510) gspI 4124691..4125065 (+) 375 WP_225762611.1 type II secretion system minor pseudopilin GspI -
  LVO07_RS19515 (LVO07_19515) gspJ 4125062..4125667 (+) 606 WP_000706931.1 type II secretion system minor pseudopilin GspJ -
  LVO07_RS19520 (LVO07_19520) gspK 4125675..4126640 (+) 966 WP_225762991.1 type II secretion system minor pseudopilin GspK -
  LVO07_RS19525 (LVO07_19525) gspL 4126633..4127214 (+) 582 WP_233073964.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55527.93 Da        Isoelectric Point: 6.6914

>NTDB_id=552629 LVO07_RS19490 WP_233073963.1 4120927..4122420(+) (pilB/pilB1) [Escherichia coli strain E69]
MAVPEETAAGTVRLPYSFSRRFNLIAWCESSLEILHVYPLSVSVLQELQRRLSVPFTLRTIGEAEFEQRLNALWQRDSSE
ARQLMEDISSAGNFFTLAEELPETEDLLESNDDAPIIKLINAMLAEAIKVGASDIHIETFEKSLVIRFRVDGTLHEMLRP
GRKLASLLVSRIKVMARLDIAEKRIPQDGRIALLLGGRAIDVRVSTMPSAWGERAVLRLLDKSQACLSLEYLGLNHTLTA
QLRQLLHRPHGIFLVTGPTGSGKSTTLYAGLQELNNHTRNILTVEDPVEYMIEGIGQTQVNNRVGMTFARGLRAILRQDP
DVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAIGAITRLQDMGIEPFLLSSSLIGMMAQRLVRTLCPDCRQAVQAN
DEEKRLMGLTEARDVILYHPVGCSACNHKGYRGRTAIHELILVDATLRNLIHRKAGEREMERYIRQYSAGIRTNGIEKVL
SGETTLREVLRVTQEEQ

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=552629 LVO07_RS19490 WP_233073963.1 4120927..4122420(+) (pilB/pilB1) [Escherichia coli strain E69]
GTGGCAGTGCCAGAGGAAACGGCCGCAGGTACCGTCCGATTACCCTATAGTTTTAGTCGTCGGTTTAACCTGATAGCCTG
GTGTGAATCATCGCTGGAGATCTTGCATGTCTATCCACTGTCAGTTTCCGTCTTGCAGGAACTACAGCGTCGATTGAGCG
TCCCATTCACACTAAGGACAATTGGTGAGGCAGAATTTGAACAGCGTCTTAATGCATTATGGCAGCGGGACTCTTCTGAG
GCTCGCCAACTTATGGAAGATATCAGTTCTGCCGGGAATTTCTTTACCCTCGCAGAGGAACTACCTGAAACCGAAGATCT
TCTGGAAAGTAACGACGATGCCCCCATCATCAAATTAATCAACGCAATGTTGGCAGAGGCAATTAAAGTGGGAGCCTCCG
ATATACATATTGAGACGTTTGAAAAGAGTCTGGTAATCCGTTTTCGTGTTGATGGAACGCTACATGAAATGTTGCGTCCC
GGACGAAAATTGGCCTCATTGCTGGTATCGCGTATTAAAGTGATGGCTCGCCTGGATATTGCCGAAAAACGTATTCCACA
GGATGGGCGAATCGCGTTGCTACTGGGCGGTCGGGCGATTGATGTACGTGTCTCCACAATGCCATCAGCCTGGGGAGAAC
GGGCGGTGCTTCGTCTGCTGGATAAAAGCCAGGCCTGCCTCTCTCTTGAGTATCTCGGACTGAACCACACTCTAACCGCG
CAACTGCGGCAACTTTTACACCGCCCCCATGGCATATTTCTGGTCACTGGGCCAACAGGTTCCGGCAAAAGCACTACGCT
CTATGCAGGTTTACAGGAGTTGAACAACCACACGCGCAACATCCTTACAGTTGAAGATCCTGTTGAGTATATGATTGAAG
GAATAGGCCAGACGCAAGTTAATAACCGCGTTGGGATGACCTTCGCCCGTGGCCTGCGGGCGATTTTACGCCAGGACCCT
GATGTCGTGATGGTTGGCGAAATTCGAGACACCGAAACGGCGGAAATTGCTGTTCAGGCTTCTCTAACAGGGCACCTGGT
CCTTTCCACCCTACATACCAACACTGCCATTGGAGCCATAACCCGTCTGCAAGATATGGGGATTGAACCATTTTTACTTT
CATCCAGTTTGATAGGAATGATGGCGCAACGTCTGGTTCGCACCCTATGTCCAGATTGCCGTCAGGCTGTTCAGGCTAAC
GATGAAGAAAAACGTCTTATGGGGCTTACCGAGGCACGTGATGTCATCCTGTACCACCCGGTGGGGTGTTCTGCATGTAA
TCACAAAGGATACAGAGGGCGAACAGCTATTCATGAATTAATTCTTGTTGACGCAACGCTACGTAATTTGATTCATCGTA
AGGCCGGTGAAAGGGAGATGGAGCGTTATATTCGGCAGTACTCCGCCGGGATCCGGACAAATGGTATCGAAAAAGTCCTT
TCTGGTGAAACGACGCTCAGGGAAGTTCTACGCGTTACTCAGGAGGAGCAGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(122-388)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

40.23

100

0.423
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.147

89.135

0.402
  pilB Legionella pneumophila strain ERS1305867

45.093

86.117

0.388
  pilB Vibrio cholerae strain A1552

48

80.483

0.386
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Vibrio campbellii strain DS40M4

47.727

79.678

0.38
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilF Thermus thermophilus HB27

45.75

80.483

0.368