Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LVO07_RS12700 Genome accession   NZ_CP089930
Coordinates   2687650..2689143 (+) Length   497 a.a.
NCBI ID   WP_222679694.1    Uniprot ID   -
Organism   Escherichia coli strain E69     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2682650..2694143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVO07_RS12680 (LVO07_12680) pilD 2683300..2684109 (+) 810 WP_225762974.1 prepilin peptidase PppA Machinery gene
  LVO07_RS12685 (LVO07_12685) gspS2 2684175..2684585 (+) 411 WP_024165221.1 type II secretion system pilot lipoprotein GspS-beta -
  LVO07_RS12690 (LVO07_12690) gspC 2684601..2685560 (+) 960 WP_233073624.1 type II secretion system protein GspC -
  LVO07_RS12695 (LVO07_12695) gspD 2685590..2687650 (+) 2061 WP_233073633.1 type II secretion system secretin GspD -
  LVO07_RS12700 (LVO07_12700) pilB/pilB1 2687650..2689143 (+) 1494 WP_222679694.1 type II secretion system ATPase GspE Machinery gene
  LVO07_RS12705 (LVO07_12705) gspF 2689143..2690366 (+) 1224 WP_225762234.1 type II secretion system inner membrane protein GspF -
  LVO07_RS12710 (LVO07_12710) gspG 2690383..2690838 (+) 456 WP_001087291.1 type II secretion system major pseudopilin GspG -
  LVO07_RS12715 (LVO07_12715) gspH 2690842..2691405 (+) 564 WP_032226244.1 type II secretion system minor pseudopilin GspH -
  LVO07_RS12720 (LVO07_12720) gspI 2691402..2691773 (+) 372 WP_000820130.1 type II secretion system minor pseudopilin GspI -
  LVO07_RS12725 (LVO07_12725) gspJ 2691770..2692375 (+) 606 WP_225762235.1 type II secretion system minor pseudopilin GspJ -
  LVO07_RS12730 (LVO07_12730) gspK 2692372..2693349 (+) 978 WP_225762236.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55228.38 Da        Isoelectric Point: 6.0089

>NTDB_id=552601 LVO07_RS12700 WP_222679694.1 2687650..2689143(+) (pilB/pilB1) [Escherichia coli strain E69]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLIRTLCPDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=552601 LVO07_RS12700 WP_222679694.1 2687650..2689143(+) (pilB/pilB1) [Escherichia coli strain E69]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGATGAGGCCGAGTTTGAACAGCGGCTGAATGCGGTCTGGCAGCGTGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGA
CCGGGGCGCAAACTGGCCTCGTTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGCCGGGCGATTGACGTGCGCGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGTTGAGCCATGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCACACGGTATCTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGGATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAT
CCGGATGTGGTGATGGTGGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCGTCATTGACCGGACACCT
GGTACTGTCAACCCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TATCTTCCAGTCTGACGGGCGTGATGGCGCAGCGGCTGATTCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGTTGGGGATTACCGATGCGCGTACCGTCACTCTGTACCATCCGCAGGGCTGTCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTAACTATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

44.75

80.483

0.36