Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   LVO07_RS12680 Genome accession   NZ_CP089930
Coordinates   2683300..2684109 (+) Length   269 a.a.
NCBI ID   WP_225762974.1    Uniprot ID   -
Organism   Escherichia coli strain E69     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2678300..2689109
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVO07_RS12675 (LVO07_12675) sslE 2678556..2683106 (+) 4551 WP_233073605.1 lipoprotein metalloprotease SslE -
  LVO07_RS12680 (LVO07_12680) pilD 2683300..2684109 (+) 810 WP_225762974.1 prepilin peptidase PppA Machinery gene
  LVO07_RS12685 (LVO07_12685) gspS2 2684175..2684585 (+) 411 WP_024165221.1 type II secretion system pilot lipoprotein GspS-beta -
  LVO07_RS12690 (LVO07_12690) gspC 2684601..2685560 (+) 960 WP_233073624.1 type II secretion system protein GspC -
  LVO07_RS12695 (LVO07_12695) gspD 2685590..2687650 (+) 2061 WP_233073633.1 type II secretion system secretin GspD -

Sequence

Protein


Download         Length: 269 a.a.        Molecular weight: 29411.00 Da        Isoelectric Point: 8.3821

>NTDB_id=552600 LVO07_RS12680 WP_225762974.1 2683300..2684109(+) (pilD) [Escherichia coli strain E69]
MHFDVFQQYPAVMPILATVGGLIIGSFLNVVIWRYPIMLRQQMAEFQGETPSAQSKISLALPRSHCPHCQQTIRVRDNIP
LFSWLMLKGRCRDCQAKISKRYPLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGWVGALSLPNVALIASCCGLIYAV
ITKKGSTTLPFGPCLSLGGIATLYLQALL

Nucleotide


Download         Length: 810 bp        

>NTDB_id=552600 LVO07_RS12680 WP_225762974.1 2683300..2684109(+) (pilD) [Escherichia coli strain E69]
ATGCATTTTGATGTTTTTCAGCAATATCCAGCGGTGATGCCCATCCTGGCAACCGTTGGAGGATTAATCATCGGCAGTTT
TTTGAATGTGGTGATTTGGCGTTACCCCATCATGCTGCGCCAACAAATGGCGGAGTTTCAGGGGGAAACTCCGAGTGCGC
AGTCAAAAATAAGCCTGGCGCTGCCACGTTCGCACTGTCCGCATTGCCAGCAGACCATCCGCGTTCGTGACAATATTCCG
CTATTCTCCTGGCTGATGCTCAAAGGGCGCTGCCGCGACTGTCAGGCGAAAATCAGCAAGCGTTATCCGCTGGTGGAGTT
ATTGACGGCACTCGCTTTTTTGCTGGCGAGTCTGGTCTGGCCGGAAAGTGGATGGGCGCTGGCGGTGATGATATTATCCG
CCTGGCTGATTGCCGCGAGTGTCATCGACCTCGATCACCAATGGCTGCCCGATGTTTTTACTCAGGGCGTATTGTGGACA
GGACTTATTGCGGCATGGGCGCAGCAGAGTCCGTTAACGCTACAAGACGCAGTCACCGGCGTCCTGGTGGGGTTTATCAC
TTTTTACTCGCTGCGCTGGATAGCCGGAATAGTTCTGCGTAAAGAAGCATTAGGCATGGGCGATGTATTACTCTTCGCCG
CTTTAGGTGGCTGGGTGGGGGCGTTGTCGCTGCCCAATGTTGCTTTAATCGCCTCATGCTGCGGCCTGATATATGCCGTT
ATTACAAAAAAAGGATCAACCACACTGCCTTTCGGACCGTGTTTAAGTCTGGGCGGTATAGCAACACTTTATCTACAGGC
ATTGCTTTAA

Domains

Predicted by InterProScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(21-118)

Type IV leader peptidase family (Peptidase_A24)

(130-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

46.067

99.257

0.457
  pilD Vibrio campbellii strain DS40M4

41.026

100

0.416
  pilD Neisseria gonorrhoeae MS11

39.535

95.911

0.379
  pilD Acinetobacter nosocomialis M2

37.736

98.513

0.372
  pilD Acinetobacter baumannii D1279779

37.358

98.513

0.368