Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J6335_RS15995 Genome accession   NZ_CP072393
Coordinates   3698775..3700484 (-) Length   569 a.a.
NCBI ID   WP_180959844.1    Uniprot ID   -
Organism   Xanthomonas phaseoli pv. phaseoli strain 6546R(delta)tal18H     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3693775..3705484
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J6335_RS15975 (J6335_15975) - 3694668..3696281 (+) 1614 WP_039568284.1 HAMP domain-containing sensor histidine kinase -
  J6335_RS15980 (J6335_15980) pilR 3696552..3698006 (+) 1455 WP_039568287.1 sigma-54 dependent transcriptional regulator Regulator
  J6335_RS15985 (J6335_15985) - 3698203..3698349 (-) 147 WP_080948799.1 serine/threonine protein kinase -
  J6335_RS15990 (J6335_15990) - 3698411..3698530 (-) 120 WP_039568289.1 hypothetical protein -
  J6335_RS15995 (J6335_15995) pilB 3698775..3700484 (-) 1710 WP_180959844.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J6335_RS16000 (J6335_16000) - 3700576..3701031 (-) 456 WP_039568290.1 pilin -
  J6335_RS16005 (J6335_16005) pilA 3701141..3701581 (-) 441 WP_039568292.1 pilin Machinery gene
  J6335_RS16010 (J6335_16010) - 3701875..3703101 (-) 1227 WP_011345585.1 IS256-like element ISXax1 family transposase -
  J6335_RS16015 (J6335_16015) pilC 3703273..3704529 (+) 1257 WP_039573972.1 type II secretion system F family protein Machinery gene
  J6335_RS16020 (J6335_16020) - 3704536..3705399 (+) 864 WP_039573976.1 A24 family peptidase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 61623.59 Da        Isoelectric Point: 5.4526

>NTDB_id=552303 J6335_RS15995 WP_180959844.1 3698775..3700484(-) (pilB) [Xanthomonas phaseoli pv. phaseoli strain 6546R(delta)tal18H]
MGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDASQNAVKLVSE
ELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALGDDEEGMGDL
DVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPVKLNQRIAA
RLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQKLFLEAIHKP
YGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPDNALLAEGF
TPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEISAIVLEGGNAMQIAEAAQAIGIRDLRQSALMKAANGVTSL
AEINRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=552303 J6335_RS15995 WP_180959844.1 3698775..3700484(-) (pilB) [Xanthomonas phaseoli pv. phaseoli strain 6546R(delta)tal18H]
GTGGGAATTACGGGCATCGCGCGCCGTCTGGTACAGGACGGCGCTGTCGAAGAGGCAGTTGCGCGATCTGCGATGGATCA
GGCATCTGCGGCAAAAGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGGTCACTGCTTCCCAGCTCGCCGCTGCTA
ATGCGGTCGAGTTCGGTATGCCGCTGCTGGATGTGTCGGCGTTCGACGCCAGCCAAAACGCGGTCAAGCTGGTGAGTGAG
GAACTACTCCAGAAGCACCAAGTCCTGCCGCTGTTCAAGCGCGGCAACCGGCTGTTCGTGGGGGTGAGCAATCCGACCCA
GACCCGGGCGCTGGATGACATCAAGTTCCACACGAACTTGGTAGTTGAGCCCATCCTTGTGGATGAAGATCAGATCCGTC
GCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCACTGGGCTCCGCGCTCGGTGACGACGAGGAGGGCATGGGGGATCTC
GACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGATGCCAAGGGCGACGACACGCCGGTGGT
GAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACATCCATTTCGAGCCGTATGAAGACGATT
ACCGGGTGCGCTTGCGCATCGACGGCTTGCTCAAGAACGTGGCCAAGGCGCCGGTGAAGCTGAATCAGCGCATCGCAGCG
CGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGATGGGCGCATCAAGCTCAACCTGTCCAA
GACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGTGAGAAGGTGGTGCTACGTATCCTGGACGGCA
GCGCGGCCAAGCTGGGTATCGACAAGCTGGGCTATGAGGCGGACCAGCAGAAGTTGTTCCTGGAAGCGATCCACAAGCCG
TACGGGATGGTACTGGTGACCGGGCCGACTGGCTCGGGCAAGACGGTCTCGCTGTACACGGCGCTGGGTATCCTCAATGA
CGAGACGCGCAATATCTCCACTGCGGAAGACCCGGTCGAAATCCGCTTGCCTGGTGTCAACCAGGTGCAGCAGAACAACA
AGCGTGGCATGACCTTCGCCGCAGCCTTGCGCTCGTTCCTGCGACAGGATCCGGACATCATCATGGTCGGCGAAATCCGC
GACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCCCAGACCGGTCACATGGTGCTGTCGACGTTGCACACCAACGATGC
GCCACAGACCATTGCGCGTCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGGTGACCCTGGTCATCGCGC
AGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCTGATAACGCATTGCTGGCCGAAGGATTC
ACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTTGGCTGCGACGAGTGCACCGAAGGCTACAAGGGGCG
GACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCAGTGCGATCGTGCTGGAAGGCGGCAATGCAATGCAGATCG
CCGAGGCCGCGCAGGCGATCGGTATCCGAGATTTGCGTCAATCGGCGCTGATGAAGGCTGCGAATGGGGTCACCAGCCTG
GCAGAGATCAACCGGGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-144)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.69

99.824

0.566
  pilB Acinetobacter baylyi ADP1

55.81

99.824

0.557
  pilB Legionella pneumophila strain ERS1305867

51.67

100

0.517
  pilB Vibrio cholerae strain A1552

50.181

97.364

0.489
  pilF Neisseria gonorrhoeae MS11

48.944

99.824

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

47.143

98.418

0.464
  pilB Vibrio campbellii strain DS40M4

45.694

100

0.457
  pilF Thermus thermophilus HB27

39.615

91.388

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.048

92.267

0.36