Detailed information NTG0550927

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: LUZ08_RS02350
• Genome accession: NZ_CP089489
• Coordinates: 410601..411605 (+)
• Length: 334 a.a.
• NCBI ID: WP_001005767.1
• Uniprot ID: Q6GG63
• Organism: Staphylococcus aureus strain E1174_IV_ST22
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 405601..416605

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LUZ08_RS02315	mreD	406211..406741 (+)	531	WP_001259925.1	rod shape-determining protein MreD	-
LUZ08_RS02320	rplU	406908..407216 (+)	309	WP_000457385.1	50S ribosomal protein L21	-
LUZ08_RS02325	-	407222..407542 (+)	321	WP_000633692.1	ribosomal-processing cysteine protease Prp	-
LUZ08_RS02330	rpmA	407554..407838 (+)	285	WP_000916187.1	50S ribosomal protein L27	-
LUZ08_RS02335	obgE	408193..409485 (+)	1293	WP_000496100.1	GTPase ObgE	-
LUZ08_RS02340	-	409498..409953 (+)	456	WP_000368902.1	ACT domain-containing protein	-
LUZ08_RS02345	ruvA	409967..410569 (+)	603	WP_000271554.1	Holliday junction branch migration protein RuvA	Machinery gene
LUZ08_RS02350	ruvB	410601..411605 (+)	1005	WP_001005767.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
LUZ08_RS02355	queA	411607..412632 (+)	1026	WP_001019172.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
LUZ08_RS02360	tgt	412655..413794 (+)	1140	WP_001112045.1	tRNA guanosine(34) transglycosylase Tgt	-
LUZ08_RS02365	yajC	413813..414073 (+)	261	WP_001160682.1	preprotein translocase subunit YajC	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 37718.00 Da
• Isoelectric Point: 5.0865

>NTDB_id=550927 LUZ08_RS02350 WP_001005767.1 410601..411605(+) (ruvB) [Staphylococcus aureus strain E1174_IV_ST22]
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERE

Nucleotide

• Length: 1005 bp

>NTDB_id=550927 LUZ08_RS02350 WP_001005767.1 410601..411605(+) (ruvB) [Staphylococcus aureus strain E1174_IV_ST22]
ATGAATGAGCGTATGGTTGATCAATCAATGCATAGTGAAGAAACTGATTTCGAATTGTCGCTTAGACCTACGAGATTACG
ACAATATATTGGTCAAAATTCAATAAAAAGTAATTTAGAAGTATTTATTAAAGCGGCTAAACTTCGTCATGAACCATTAG
ATCATGTATTGCTTTTTGGCCCCCCTGGATTAGGTAAGACAACATTATCTAATATCATTGCCAATGAAATGGAAGTTAAT
ATACGTACAGTATCAGGGCCTTCATTAGAAAGACCTGGTGATTTGGCTGCAATTTTATCAGGACTTCAACCTGGAGATGT
TTTGTTTATTGATGAAATACACAGACTGAGTAGTGTTGTTGAAGAAGTGTTATACCCTGCAATGGAAGATTTCTTTTTAG
ATATTATCATTGGTAAAGGCGATGAGGCTAGAAGTATCCGTATCGACTTACCTCCATTCACTTTGGTAGGTGCAACAACG
CGAGCTGGCAGCTTAACAGGTCCACTAAGGGATCGATTTGGTGTGCACTTAAGATTAGAATATTATAACGAATCAGATTT
AAAAGAAATCATTATTAGAACAGCTGAGGTTTTAGGCACAGGTATTGATGAAGAAAGTGCCATTGAACTTGCTAAACGTT
CTAGAGGGACTCCAAGAGTAGCAAATCGACTATTGAAGCGGGTAAGAGACTTCCAGCAAGTGAATGAAGATGAACAAATA
TACATTGAAACAACGAAGCACGCATTAGGTTTACTTCAAGTTGATCAACACGGACTAGATTACATTGATCATAAAATGAT
GAACTGTATTATTAAGCAGTATAATGGCGGACCTGTTGGTTTAGATACGATTGCCGTAACAATTGGTGAAGAACGTATTA
CAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCA
TTAGCTTATGAACATTTTGCAAAGTCGAATGAGGAGAGAGAATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (182-255)

• RuvB C-terminal winged helix domain (WHD_RuvB) (257-326)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (21-179)

3D structure

• AlphaFold DB: Q6GG63

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	65.951	97.605	0.644
ruvB	Streptococcus pneumoniae TIGR4	58.182	98.802	0.575
ruvB	Streptococcus pneumoniae R6	57.879	98.802	0.572
ruvB	Streptococcus pneumoniae D39	57.879	98.802	0.572
ruvB	Helicobacter pylori 26695	56.481	97.006	0.548
ruvB	Synechocystis sp. PCC 6803	53.75	95.808	0.515