Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   J4Q32_RS10245 Genome accession   NZ_CP071916
Coordinates   1953120..1954061 (-) Length   313 a.a.
NCBI ID   WP_000249567.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 19A-19087     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1948120..1959061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4Q32_RS10215 (J4Q32_10220) - 1948368..1948643 (-) 276 WP_050202804.1 hypothetical protein -
  J4Q32_RS10220 (J4Q32_10225) - 1948804..1949556 (+) 753 WP_233922969.1 XRE family transcriptional regulator -
  J4Q32_RS10225 (J4Q32_10230) - 1949558..1950322 (+) 765 WP_219576745.1 hypothetical protein -
  J4Q32_RS10230 (J4Q32_10235) - 1950629..1952074 (+) 1446 WP_219576746.1 recombinase family protein -
  J4Q32_RS10240 (J4Q32_10245) comGB/cglB 1952156..1953172 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  J4Q32_RS10245 (J4Q32_10250) comGA/cglA/cilD 1953120..1954061 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  J4Q32_RS10250 (J4Q32_10255) - 1954137..1954502 (-) 366 WP_000286415.1 DUF1033 family protein -
  J4Q32_RS10255 (J4Q32_10260) - 1954653..1955711 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  J4Q32_RS10260 (J4Q32_10265) nagA 1955874..1957025 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  J4Q32_RS10265 (J4Q32_10270) - 1957178..1958995 (-) 1818 WP_001220865.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35573.48 Da        Isoelectric Point: 6.0083

>NTDB_id=548984 J4Q32_RS10245 WP_000249567.1 1953120..1954061(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 19A-19087]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=548984 J4Q32_RS10245 WP_000249567.1 1953120..1954061(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain 19A-19087]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGGCTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCATGCTAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTTTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.361

100

0.994
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.361

100

0.994
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.486

100

0.965
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361