Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF/cglF   Type   Machinery gene
Locus tag   J4Q32_RS09915 Genome accession   NZ_CP071916
Coordinates   1909953..1910414 (-) Length   153 a.a.
NCBI ID   WP_000250545.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 19A-19087     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1904953..1915414
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4Q32_RS09885 (J4Q32_09875) - 1905264..1906088 (-) 825 WP_000727904.1 membrane protein insertase YidC -
  J4Q32_RS09890 (J4Q32_09880) rnpA 1906063..1906434 (-) 372 WP_000739246.1 ribonuclease P protein component -
  J4Q32_RS11355 - 1906451..1906582 (-) 132 WP_000768904.1 hypothetical protein -
  J4Q32_RS09895 (J4Q32_09885) - 1906583..1907773 (-) 1191 WP_000167766.1 acetate kinase -
  J4Q32_RS09900 (J4Q32_09890) comYH 1907824..1908777 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  J4Q32_RS09905 (J4Q32_09895) - 1908838..1909425 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  J4Q32_RS09910 (J4Q32_09900) comGG/cglG 1909562..1909975 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  J4Q32_RS09915 (J4Q32_09905) comGF/cglF 1909953..1910414 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  J4Q32_RS09920 (J4Q32_09910) comGE/cglE 1910377..1910679 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  J4Q32_RS09925 (J4Q32_09915) comGD/cglD 1910642..1911046 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  J4Q32_RS09930 (J4Q32_09920) comGC/cglC 1911039..1911305 (-) 267 WP_000962026.1 competence type IV pilus major pilin ComGC Machinery gene
  J4Q32_RS09935 (J4Q32_09925) - 1911307..1911564 (-) 258 WP_000698513.1 hypothetical protein -
  J4Q32_RS09940 (J4Q32_09930) - 1911796..1912752 (-) 957 WP_233923013.1 N-acetylmuramoyl-L-alanine amidase family protein -
  J4Q32_RS09945 (J4Q32_09935) - 1912755..1913090 (-) 336 WP_050200954.1 phage holin -
  J4Q32_RS09950 (J4Q32_09940) - 1913094..1913510 (-) 417 WP_001165344.1 phage holin family protein -
  J4Q32_RS09955 (J4Q32_09945) - 1913520..1913870 (-) 351 WP_050200952.1 hypothetical protein -
  J4Q32_RS09960 (J4Q32_09950) - 1913873..1914076 (-) 204 WP_001091109.1 hypothetical protein -
  J4Q32_RS11515 - 1914057..1914173 (-) 117 WP_001063633.1 hypothetical protein -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 17855.34 Da        Isoelectric Point: 6.4832

>NTDB_id=548977 J4Q32_RS09915 WP_000250545.1 1909953..1910414(-) (comGF/cglF) [Streptococcus pneumoniae strain 19A-19087]
MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVEGN
RFYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide


Download         Length: 462 bp        

>NTDB_id=548977 J4Q32_RS09915 WP_000250545.1 1909953..1910414(-) (comGF/cglF) [Streptococcus pneumoniae strain 19A-19087]
ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTGCT
CGTCATCAGTGGAGGCTTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG
AGCAAAAGGAGTGGCTCTTGTTTGTGGATCAACTTGAGGCAGAATTAGACCGTTCGCAGTTCGAAAAAGTGGAAGGCAAT
CGTTTCTATATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG
TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT
TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF/cglF Streptococcus pneumoniae Rx1

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae D39

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae R6

93.464

100

0.935
  comGF/cglF Streptococcus pneumoniae TIGR4

93.464

100

0.935
  comGF/cglF Streptococcus mitis SK321

92.81

100

0.928
  comGF/cglF Streptococcus mitis NCTC 12261

91.503

100

0.915