Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J4377_RS09425 Genome accession   NZ_CP071889
Coordinates   2018481..2020235 (+) Length   584 a.a.
NCBI ID   WP_253488050.1    Uniprot ID   -
Organism   Halomonas sp. XH26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2013481..2025235
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4377_RS09395 (J4377_09380) - 2013939..2014298 (+) 360 WP_253488038.1 hypothetical protein -
  J4377_RS09400 (J4377_09385) - 2014554..2015771 (-) 1218 WP_009724493.1 EAL and HDOD domain-containing protein -
  J4377_RS09410 (J4377_09395) - 2016348..2017157 (-) 810 WP_253488039.1 inositol monophosphatase family protein -
  J4377_RS09415 (J4377_09400) - 2017252..2017707 (-) 456 WP_253488041.1 anti-virulence regulator CigR family protein -
  J4377_RS18835 (J4377_09405) - 2017871..2018266 (-) 396 WP_301271693.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  J4377_RS09425 (J4377_09410) pilB 2018481..2020235 (+) 1755 WP_253488050.1 type IV-A pilus assembly ATPase PilB Machinery gene
  J4377_RS09430 (J4377_09415) - 2020242..2021471 (+) 1230 WP_253488052.1 type II secretion system F family protein -
  J4377_RS09435 (J4377_09420) pilD 2021527..2022372 (+) 846 WP_038484644.1 A24 family peptidase Machinery gene
  J4377_RS09440 (J4377_09425) coaE 2022414..2023010 (+) 597 WP_253488054.1 dephospho-CoA kinase -
  J4377_RS09445 (J4377_09430) yacG 2023050..2023283 (+) 234 WP_038484650.1 DNA gyrase inhibitor YacG -
  J4377_RS09450 (J4377_09435) - 2023297..2024169 (+) 873 WP_038484653.1 DNA replication terminus site-binding protein -
  J4377_RS09455 (J4377_09440) - 2024197..2024544 (-) 348 WP_253488056.1 hypothetical protein -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63793.23 Da        Isoelectric Point: 5.9361

>NTDB_id=548818 J4377_RS09425 WP_253488050.1 2018481..2020235(+) (pilB) [Halomonas sp. XH26]
MNDLPPRPTVSKAAMHSGLHGIAKRLIEHGVLTEAQATVAESTATELDVSLLQHVVDSGLVESGAAALAAAWEYGLPVVD
LDAIRINALPPASDYPVKILERLDVLPLARHGHRLTVAVPYPATLTQLNELQFATGLSVDGVLAPADQLKNTLTQYLAQN
ERSMLDELENVDDAVSALSIVQLDEDEATLDKAVTDSEDAPVVKFVNKILLDAISLGASDIHFEPYETQYRVRFRIDGML
LEIARPPFALRHRIAARVKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVFGEKMVLRLLDPASAQLAIEQLG
FSPEQQAVYEHALKKPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVPGVNQVNVLPKIGLNFASALR
AFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGIASYNIASSVSLIIAQRLARKLCNLC
KTPAKIPAEALHQAGFSQQEIRNGTIYSAVGCKQCTQGYKGRIGIYEVVPITDAMRQLIMRDGDALDIDQQARREGYPSL
YQRCLHRVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=548818 J4377_RS09425 WP_253488050.1 2018481..2020235(+) (pilB) [Halomonas sp. XH26]
ATGAACGATTTACCCCCCAGGCCGACGGTGAGCAAGGCGGCCATGCATAGCGGCCTGCACGGTATCGCTAAACGGCTGAT
AGAACATGGCGTGCTGACCGAAGCCCAGGCGACTGTCGCGGAAAGCACAGCAACAGAGCTTGACGTGTCGCTGTTACAGC
ACGTGGTCGATAGTGGGCTGGTAGAATCAGGCGCTGCAGCGCTGGCCGCTGCCTGGGAGTATGGTTTACCGGTTGTTGAT
CTAGATGCCATTCGTATAAACGCGCTACCACCCGCCAGCGACTACCCCGTTAAAATATTAGAGCGTCTAGATGTTCTTCC
CCTGGCACGCCACGGGCACCGCCTTACTGTCGCTGTACCCTACCCCGCTACGCTAACCCAACTGAATGAACTTCAATTTG
CTACCGGGCTAAGTGTAGATGGAGTACTGGCTCCCGCTGATCAACTTAAAAACACTCTAACGCAGTATCTAGCGCAAAAT
GAGCGCAGCATGCTAGATGAACTGGAAAATGTAGATGATGCGGTCAGCGCGTTGAGTATTGTTCAGCTTGACGAAGATGA
GGCGACATTAGACAAGGCAGTAACAGATAGCGAAGATGCCCCCGTCGTTAAATTCGTCAATAAAATACTGCTAGATGCTA
TCAGTCTCGGCGCTTCCGATATTCACTTTGAGCCCTATGAAACCCAGTACCGGGTGCGCTTTCGTATTGATGGCATGCTG
CTTGAAATTGCTCGCCCACCTTTTGCGCTGCGGCACCGCATCGCGGCACGTGTAAAAATCATGTCTCGGTTGGATATCTC
TGAGCGGCGCCTGCCTCAGGATGGTGCAATCAAGCTGCAGCTTTCACGCACACGCTCAATTGATTTTCGAGTGAACTCAC
TGCCCACTGTGTTTGGTGAAAAAATGGTACTGCGTTTGCTAGATCCTGCCTCTGCTCAACTCGCTATCGAGCAGCTAGGC
TTTAGCCCTGAACAGCAGGCCGTTTATGAACACGCGCTTAAGAAGCCTCAAGGAATGATTCTGGTTACCGGGCCTACGGG
GAGCGGTAAAACAGTGACGCTTTATACCGGCATCAACATTCTTAACCAAATTGAGCGCAATATCTGCACCGCCGAGGACC
CGGTAGAAATTAAAGTGCCGGGGGTCAATCAGGTGAACGTTCTCCCTAAAATCGGTCTGAACTTCGCCAGCGCTCTGCGC
GCCTTTTTACGCCAAGACCCTGATGTTGTCATGGTGGGCGAAATTCGCGACTTGGAAACCGCCGAAATTGCCGTAAAAGC
AGCCCAAACAGGTCACTTAGTGCTATCTACCGTACACACTAACTCCGCCGCTGAAACGCTAACCCGATTAGCGAATATGG
GCATCGCATCGTATAACATCGCAAGCTCAGTTAGCTTGATTATTGCCCAGCGCTTGGCACGCAAGCTATGCAACCTATGC
AAAACACCTGCAAAGATTCCTGCCGAAGCGCTGCATCAGGCTGGCTTTAGCCAACAGGAGATTCGCAACGGCACGATTTA
TTCAGCGGTCGGCTGTAAACAGTGCACACAGGGCTATAAAGGTCGTATTGGTATCTATGAAGTGGTGCCTATTACTGATG
CCATGCGCCAGCTGATTATGCGCGATGGCGATGCGCTGGATATCGACCAACAAGCGCGCCGCGAAGGTTACCCTAGCCTT
TATCAGCGTTGCCTACATCGTGTGCTGGATGGCAGCACTAGTCTGGAAGAAGTTAACCGCATCAGTAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-161)

Type II/IV secretion system protein (T2SSE)

(208-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.212

96.747

0.505
  pilB Acinetobacter baylyi ADP1

48.772

97.603

0.476
  pilB Acinetobacter baumannii D1279779

48.09

98.63

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

47.11

97.774

0.461
  pilB Vibrio campbellii strain DS40M4

47.007

97.26

0.457
  pilB Vibrio cholerae strain A1552

51.172

87.671

0.449
  pilF Neisseria gonorrhoeae MS11

49.895

81.678

0.408
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.986

96.918

0.368