Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   J4N44_RS15165 Genome accession   NZ_CP071852
Coordinates   3188756..3189349 (-) Length   197 a.a.
NCBI ID   WP_005451500.1    Uniprot ID   A0A9X3S2A2
Organism   Vibrio sp. SCSIO 43155     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3183756..3194349
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N44_RS15145 (J4N44_15075) aroB 3184625..3185725 (-) 1101 WP_009697563.1 3-dehydroquinate synthase -
  J4N44_RS15150 (J4N44_15080) aroK 3185757..3186275 (-) 519 WP_005451507.1 shikimate kinase AroK -
  J4N44_RS15155 (J4N44_15085) pilQ 3186465..3188216 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N44_RS15160 (J4N44_15090) pilP 3188251..3188766 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  J4N44_RS15165 (J4N44_15095) pilO 3188756..3189349 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N44_RS15170 (J4N44_15100) pilN 3189342..3189917 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  J4N44_RS15175 (J4N44_15105) pilM 3189901..3190917 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene
  J4N44_RS15180 (J4N44_15110) - 3191096..3193666 (+) 2571 WP_050902270.1 PBP1A family penicillin-binding protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23020.58 Da        Isoelectric Point: 4.8068

>NTDB_id=548384 J4N44_RS15165 WP_005451500.1 3188756..3189349(-) (pilO) [Vibrio sp. SCSIO 43155]
MVNWQDLEMDEIAEWPLVPQIVVILLLVVLIQGLGYWFYLKPEQEQLNNLIQEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKQSEHFLYRLPLDIELTGDFHDIGRFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVKANTYQFKPEVKKDEK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=548384 J4N44_RS15165 WP_005451500.1 3188756..3189349(-) (pilO) [Vibrio sp. SCSIO 43155]
ATGGTTAATTGGCAAGATCTAGAAATGGATGAGATCGCTGAGTGGCCATTGGTGCCTCAGATAGTAGTGATCTTGTTGTT
AGTTGTGCTGATTCAAGGCCTTGGTTATTGGTTTTATTTAAAGCCAGAGCAAGAACAGCTGAATAACCTTATTCAAGAAG
AACAAACCTTAAAAGTTGCGTTACGGATCAAAGCGAACAAAGTCGCTGCTTTGCCTCAATTGCAATCGCAACTTGATGAG
CTTACTACTCGATACGAATTGCTACTGCAACAACTGCCTGCACAGAAAGAGCTGGCAACCATGTTGGCTTCGGTGAATGA
ACTCGGAATTGATAATAAGTTGACGTTCACCCGAATCGACTGGGGTGATAAGCAATCAGAACACTTCCTTTATCGTTTAC
CGCTCGACATTGAGTTGACAGGGGATTTTCATGATATCGGTCGTTTCTCCCAAGCGATTGCGACGTTGCCACGCATCATC
ACTTTTAAAGACGTGACTTGGCAGCGTGTCAGCCAAGAGAGTGAAACTCTGCACTTTCGTGTGAAAGCAAACACTTACCA
GTTCAAACCAGAGGTGAAGAAAGATGAGAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

93.158

96.447

0.898
  pilO Vibrio cholerae strain A1552

71.066

100

0.711