Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   J4N44_RS15175 Genome accession   NZ_CP071852
Coordinates   3189901..3190917 (-) Length   338 a.a.
NCBI ID   WP_026000383.1    Uniprot ID   A0A3A1Q0R6
Organism   Vibrio sp. SCSIO 43155     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3184901..3195917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J4N44_RS15150 (J4N44_15080) aroK 3185757..3186275 (-) 519 WP_005451507.1 shikimate kinase AroK -
  J4N44_RS15155 (J4N44_15085) pilQ 3186465..3188216 (-) 1752 WP_005451505.1 type IV pilus secretin PilQ family protein Machinery gene
  J4N44_RS15160 (J4N44_15090) pilP 3188251..3188766 (-) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  J4N44_RS15165 (J4N44_15095) pilO 3188756..3189349 (-) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  J4N44_RS15170 (J4N44_15100) pilN 3189342..3189917 (-) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  J4N44_RS15175 (J4N44_15105) pilM 3189901..3190917 (-) 1017 WP_026000383.1 type IV pilus assembly protein PilM Machinery gene
  J4N44_RS15180 (J4N44_15110) - 3191096..3193666 (+) 2571 WP_050902270.1 PBP1A family penicillin-binding protein -
  J4N44_RS15185 (J4N44_15115) oxyR 3193733..3194641 (-) 909 WP_005451495.1 DNA-binding transcriptional regulator OxyR -
  J4N44_RS15190 (J4N44_15120) - 3194816..3195544 (+) 729 WP_005451494.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38066.43 Da        Isoelectric Point: 6.4206

>NTDB_id=548386 J4N44_RS15175 WP_026000383.1 3189901..3190917(-) (pilM) [Vibrio sp. SCSIO 43155]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDSQVATEQFN
RQIVDRVKRQIQLYTSINGNQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=548386 J4N44_RS15175 WP_026000383.1 3189901..3190917(-) (pilM) [Vibrio sp. SCSIO 43155]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGATAATGCC
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCAACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGCACTTCAAATGATCTATTTCAACAAGACCCTCTTGCTGAAGACTCGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGAAATCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3A1Q0R6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.533

100

0.905
  pilM Vibrio cholerae strain A1552

48.83

100

0.494