NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	LSI69_RS02800	Genome accession	NZ_CP089032
Coordinates	563707..564831 (+)	Length	374 a.a.
NCBI ID	WP_000228544.1	Uniprot ID	Q8X8F4
Organism	Escherichia coli O157:H7 strain 6535WT
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 558707..569831

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSI69_RS02775 (LSI69_02775)	mscL	558833..559243 (-)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-
LSI69_RS02780 (LSI69_02780)	trkA	559373..560749 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
LSI69_RS02785 (LSI69_02785)	rsmB	560771..562060 (-)	1290	WP_001301795.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
LSI69_RS02790 (LSI69_02790)	fmt	562106..563053 (-)	948	WP_000004459.1	methionyl-tRNA formyltransferase	-
LSI69_RS02795 (LSI69_02795)	def	563068..563577 (-)	510	WP_000114984.1	peptide deformylase	-
LSI69_RS02800 (LSI69_02800)	dprA	563707..564831 (+)	1125	WP_000228544.1	DNA-protecting protein DprA	Machinery gene
LSI69_RS02805 (LSI69_02805)	smg	564803..565276 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
LSI69_RS02810 (LSI69_02810)	yrdD	565305..565847 (+)	543	WP_001129722.1	type I DNA topoisomerase	-
LSI69_RS02815 (LSI69_02815)	tsaC	565852..566424 (+)	573	WP_001301437.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
LSI69_RS02820 (LSI69_02820)	aroE	566429..567247 (+)	819	WP_000451254.1	shikimate dehydrogenase	-
LSI69_RS02825 (LSI69_02825)	yrdB	567244..567501 (+)	258	WP_001070590.1	DUF1488 domain-containing protein	-
LSI69_RS02830 (LSI69_02830)	yrdA	567477..568031 (-)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 41005.96 Da Isoelectric Point: 6.2655

                                >NTDB_id=547771 LSI69_RS02800 WP_000228544.1 563707..564831(+) (dprA) [Escherichia coli O157:H7 strain 6535WT]

MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=547771 LSI69_RS02800 WP_000228544.1 563707..564831(+) (dprA) [Escherichia coli O157:H7 strain 6535WT]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT

TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	Q8X8F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Glaesserella parasuis strain SC1401	45.401	90.107	0.409
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.545	88.235	0.393

Detailed information NTG0547771