Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   J2887_RS17295 Genome accession   NZ_CP071785
Coordinates   3777326..3778390 (-) Length   354 a.a.
NCBI ID   WP_227172350.1    Uniprot ID   -
Organism   Marinobacter sp. CA1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3772326..3783390
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J2887_RS17270 (J2887_17215) aroK 3772767..3773333 (-) 567 WP_227172344.1 shikimate kinase AroK -
  J2887_RS17275 (J2887_17220) pilQ 3773456..3775534 (-) 2079 WP_227176162.1 type IV pilus secretin PilQ Machinery gene
  J2887_RS17280 (J2887_17225) - 3775601..3776149 (-) 549 WP_227172346.1 pilus assembly protein PilP -
  J2887_RS17285 (J2887_17230) - 3776152..3776763 (-) 612 WP_227172347.1 type 4a pilus biogenesis protein PilO -
  J2887_RS17290 (J2887_17235) - 3776760..3777326 (-) 567 WP_227172349.1 PilN domain-containing protein -
  J2887_RS17295 (J2887_17240) pilM 3777326..3778390 (-) 1065 WP_227172350.1 pilus assembly protein PilM Machinery gene
  J2887_RS17300 (J2887_17245) - 3778576..3781014 (+) 2439 WP_416381871.1 penicillin-binding protein 1A -
  J2887_RS17305 (J2887_17250) - 3781117..3782382 (-) 1266 WP_227172352.1 malic enzyme-like NAD(P)-binding protein -
  J2887_RS17310 (J2887_17255) rpmE 3782691..3782912 (-) 222 WP_227172354.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37943.87 Da        Isoelectric Point: 4.1785

>NTDB_id=547767 J2887_RS17295 WP_227172350.1 3777326..3778390(-) (pilM) [Marinobacter sp. CA1]
MFGLFGKKSSAVLGVDISSSSVKLIELSKQGDRFKVESYAVEPLPANAVVEKNITDVEAVGEAVKKVAAKSRTSVKQTAV
AVSGSAVITKVIQMNGGLNEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDQVDVLLAACRKENVDVREDALDI
ASLTTKVVDVEAYALERAYTLIESQLESHGEELVVAVVDIGATMTTLSVLADGHTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYESEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTNTPTLVANP
FADMAVGSRVNASALGNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=547767 J2887_RS17295 WP_227172350.1 3777326..3778390(-) (pilM) [Marinobacter sp. CA1]
GTGTTCGGATTGTTTGGAAAAAAATCCAGTGCAGTACTGGGTGTCGATATTAGCTCCAGCTCGGTGAAGTTGATTGAACT
CTCTAAGCAGGGGGATCGCTTCAAGGTCGAGAGCTACGCCGTTGAGCCGCTCCCGGCCAATGCGGTGGTGGAGAAGAACA
TCACCGATGTTGAGGCCGTAGGAGAGGCTGTCAAGAAGGTGGCTGCCAAGTCCCGCACCAGCGTCAAGCAGACGGCGGTG
GCGGTGTCCGGTTCAGCTGTGATCACCAAGGTGATCCAGATGAACGGTGGCCTTAACGAATTTGAGATGGAAGACCAGAT
TGCCCTTGAGGCGGATCAGTATATTCCGTATCCGCTGGACGAAGTGGCGATTGATTTTGAGGTTCAGGGACCGTCCGAGA
GCAATCCGGATCAGGTCGATGTTCTGCTGGCTGCCTGCCGGAAAGAGAATGTGGATGTCCGTGAGGATGCTCTGGACATC
GCCTCTCTGACCACCAAGGTTGTGGACGTGGAAGCCTACGCTCTGGAGCGGGCCTACACTCTGATTGAATCCCAGCTCGA
GTCCCATGGCGAAGAGCTGGTGGTAGCCGTGGTGGACATAGGCGCAACGATGACCACACTGAGTGTGTTGGCGGATGGTC
ACACCGTGTATACCCGCGAGCAGATTTTTGGTGGCAAGCAGTTGACCGAGGAAATCCAGCGCCGTTACGGCCTGTCTCTG
GAGGAGGCCGGACTGGCCAAGAAGCAGGGCGGTCTGCCCGATGACTATGAGTCGGAGGTGCTGACCCCGTTCCGCGAGGC
GGTGGTGCAACAGGTCGCTCGGGCCTTGCAGTTCTTCTTCGGTGCAAGCCAGTACAATGCCGTGGACTACGTGGTCCTGG
CTGGCGGTACTGCCTCGATTCAGGGACTGACGGAAATGGTGGAAGAGAAGACAAACACTCCGACCCTGGTGGCCAACCCG
TTTGCGGATATGGCGGTAGGCTCCCGGGTCAATGCATCGGCACTCGGTAATGACGCGCCGTCACTGATGATTGCTTGCGG
CCTGGCAATGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.695

100

0.517
  comM Acinetobacter nosocomialis M2

51.695

100

0.517
  comM Acinetobacter baylyi ADP1

49.435

100

0.494
  pilM Legionella pneumophila strain ERS1305867

45.48

100

0.455