Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   J0X00_RS09540 Genome accession   NZ_CP071708
Coordinates   737126..738934 (+) Length   602 a.a.
NCBI ID   WP_282477974.1    Uniprot ID   -
Organism   Vibrio sp. ABG19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 732126..743934
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J0X00_RS09510 (J0X00_09510) dsbC 732590..733351 (-) 762 WP_136486105.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
  J0X00_RS09515 (J0X00_09515) xerD 733367..734275 (-) 909 WP_136486103.1 site-specific tyrosine recombinase XerD -
  J0X00_RS09520 (J0X00_09520) fldB 734413..734934 (+) 522 WP_282477971.1 flavodoxin FldB -
  J0X00_RS09525 (J0X00_09525) ampD 734964..735512 (-) 549 WP_282477972.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  J0X00_RS09530 (J0X00_09530) nadC 735655..736542 (+) 888 WP_282477973.1 carboxylating nicotinate-nucleotide diphosphorylase -
  J0X00_RS09535 (J0X00_09535) - 736716..737126 (+) 411 WP_218562707.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  J0X00_RS09540 (J0X00_09540) pilB 737126..738934 (+) 1809 WP_282477974.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  J0X00_RS09545 (J0X00_09545) pilC 738945..740162 (+) 1218 WP_282477975.1 type II secretion system F family protein Machinery gene
  J0X00_RS09550 (J0X00_09550) pilD 740213..741082 (+) 870 WP_218562705.1 A24 family peptidase Machinery gene
  J0X00_RS09555 (J0X00_09555) coaE 741085..741693 (+) 609 WP_218562704.1 dephospho-CoA kinase -
  J0X00_RS09560 (J0X00_09560) zapD 741725..742465 (+) 741 WP_136486084.1 cell division protein ZapD -
  J0X00_RS09565 (J0X00_09565) yacG 742502..742699 (+) 198 WP_136486081.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 67040.68 Da        Isoelectric Point: 6.3908

>NTDB_id=547137 J0X00_RS09540 WP_282477974.1 737126..738934(+) (pilB) [Vibrio sp. ABG19]
MSTQLLRQLRQAGVLDGEQETALQRQTLSRHISAPQAILEQGVLTPAELSQQLSRLFSLPLIELDDFDYPALCQQLGLSE
LIIRYQALPLQRDSRELTLAVSDPGWPQAEEEFCFATGLMVKPVLTDQPSLQAAIRRLYGETLPSYHAERREEISQEQLA
DAVQVAEDEANYQLDVSQDEAPVSRFIHQVLLDAVRKRASDIHFEPYESSYRIRIRCDGILIQTQQPAPHLSRRLAARLK
ILARLDIAQRRLPQDGRIKLRLNNDSAIDLRVSTLPTLWGEKIVLRLLDNQALPLELDQLGYSKQQKQCYLQALHKPQGM
ILITGPTGSGKTISLYCGLQQLNREHLNIATAEDPVEINLNGINQVQVQPAIGFGFAQALRTFLRQDPDVLMVGEIRDRE
TADVALKAAQTGHLVLSTLHTNSAAETLIRLLNMGIEPFNLASSLNLIVAQRLVRRLCPQCKIATQLTRAQADLLHIESL
HIESRPLTHSPAASAPAHSAQEQQQQTTEQRKSVTDTVIFTANPNGCPHCNRGYAGRTGIYEMLNVEPVIADALLQRAPL
KQLESLACQQGMQTLQQAGCEKLLAGQTSLAELQRVLGLATW

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=547137 J0X00_RS09540 WP_282477974.1 737126..738934(+) (pilB) [Vibrio sp. ABG19]
ATGAGCACCCAATTACTCCGCCAGTTGCGTCAGGCCGGCGTCCTTGATGGCGAGCAGGAAACAGCGCTGCAGCGCCAGAC
GCTGAGCCGCCATATCAGTGCACCACAAGCGATCCTTGAACAAGGCGTGCTGACTCCGGCCGAACTCAGCCAGCAACTGA
GTCGTCTGTTCAGCCTGCCGCTGATTGAACTCGACGACTTTGACTACCCGGCTTTGTGTCAGCAACTGGGCCTGAGCGAG
CTGATCATTCGTTATCAGGCGCTGCCCCTGCAGCGTGACAGTCGGGAGTTAACCCTGGCCGTATCGGATCCTGGCTGGCC
CCAAGCGGAAGAAGAGTTCTGTTTTGCCACCGGTCTGATGGTTAAACCGGTGCTGACTGATCAGCCCAGCCTGCAAGCGG
CGATACGACGTCTGTATGGCGAGACACTCCCCAGCTATCATGCCGAGCGCCGCGAAGAGATCAGCCAGGAACAGCTCGCC
GATGCGGTACAGGTTGCCGAAGATGAAGCCAATTATCAGTTGGATGTCAGCCAGGACGAAGCACCGGTCAGCCGATTCAT
TCATCAGGTGTTACTCGACGCGGTACGCAAACGGGCCTCGGATATCCATTTTGAACCTTACGAGTCGAGTTATCGCATCC
GCATTCGCTGTGACGGCATCTTGATTCAGACCCAGCAACCGGCTCCGCACCTCAGTCGCCGCCTGGCAGCGCGTCTGAAA
ATTCTTGCCCGGCTGGATATCGCCCAGCGCCGTCTGCCGCAAGATGGTCGGATCAAACTGCGCCTCAATAATGACAGCGC
GATTGACCTGCGTGTATCCACTCTGCCGACCTTATGGGGCGAAAAGATCGTCCTGCGCCTGCTCGATAATCAGGCTCTGC
CGCTTGAACTGGACCAGCTTGGTTACAGCAAGCAGCAAAAACAGTGCTACCTGCAGGCTCTACACAAACCACAGGGCATG
ATCCTGATCACCGGACCAACCGGCAGCGGTAAGACCATTTCGTTATACTGCGGGCTGCAGCAGCTCAATCGTGAACACCT
CAATATCGCTACCGCAGAAGATCCGGTCGAGATCAACCTCAACGGGATTAACCAGGTCCAAGTCCAGCCGGCGATCGGAT
TTGGCTTTGCCCAGGCTCTGCGTACATTTCTGCGTCAGGATCCGGATGTACTCATGGTGGGTGAGATCCGCGATCGCGAG
ACCGCAGACGTTGCACTCAAAGCCGCTCAGACCGGGCATCTGGTCCTATCCACTCTGCATACCAATTCAGCCGCAGAGAC
TTTGATTCGGTTACTGAATATGGGCATTGAACCTTTTAATCTGGCATCGTCGCTGAATCTTATCGTGGCTCAGCGTCTGG
TACGCCGCTTATGCCCGCAGTGTAAAATCGCCACCCAACTGACCCGGGCGCAGGCCGACTTACTTCATATAGAAAGCCTT
CATATAGAAAGCCGTCCTCTAACCCACTCTCCTGCCGCAAGCGCGCCGGCTCATTCAGCACAAGAGCAGCAGCAACAGAC
AACTGAGCAGCGCAAGTCGGTAACAGACACGGTGATCTTTACCGCCAACCCGAACGGCTGTCCCCACTGTAATCGTGGTT
ACGCCGGACGCACCGGTATCTATGAAATGCTGAATGTTGAACCTGTCATTGCCGATGCACTGCTACAGCGCGCACCTCTG
AAGCAGCTGGAGTCGCTGGCTTGTCAGCAGGGCATGCAGACTTTGCAGCAAGCCGGCTGTGAAAAACTGCTCGCCGGGCA
GACCAGCCTGGCGGAGCTGCAGCGGGTACTGGGTCTGGCGACCTGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(189-456)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

60.469

99.169

0.6
  pilB Vibrio parahaemolyticus RIMD 2210633

58.961

99.169

0.585
  pilB Vibrio campbellii strain DS40M4

57.525

99.336

0.571
  pilB Acinetobacter baylyi ADP1

43.121

99.003

0.427
  pilB Acinetobacter baumannii D1279779

44.902

92.857

0.417
  pilF Neisseria gonorrhoeae MS11

40.568

99.502

0.404
  pilB Legionella pneumophila strain ERS1305867

39.832

98.837

0.394