Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   RIV7116_RS03325 Genome accession   NC_019678
Coordinates   871257..872366 (-) Length   369 a.a.
NCBI ID   WP_015116854.1    Uniprot ID   K9R730
Organism   Rivularia sp. PCC 7116     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 866257..877366
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RIV7116_RS03310 (Riv7116_0683) - 867231..869558 (-) 2328 WP_015116851.1 type IV pilus secretin family protein -
  RIV7116_RS03315 (Riv7116_0684) - 869744..870496 (-) 753 WP_015116852.1 hypothetical protein -
  RIV7116_RS03320 (Riv7116_0685) - 870493..871257 (-) 765 WP_015116853.1 PilN domain-containing protein -
  RIV7116_RS03325 (Riv7116_0686) pilM 871257..872366 (-) 1110 WP_015116854.1 type IV pilus assembly protein PilM Machinery gene
  RIV7116_RS03330 (Riv7116_0687) - 873333..874619 (-) 1287 WP_044291449.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  RIV7116_RS03335 (Riv7116_0688) - 874818..874943 (-) 126 WP_015116856.1 photosystem II protein Y -
  RIV7116_RS03340 (Riv7116_0689) - 875122..875649 (-) 528 WP_015116857.1 gamma carbonic anhydrase family protein -
  RIV7116_RS03345 (Riv7116_0690) - 875983..876498 (+) 516 WP_015116858.1 TIGR02652 family protein -
  RIV7116_RS03350 (Riv7116_0691) - 876591..877031 (+) 441 WP_015116859.1 VOC family protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40321.25 Da        Isoelectric Point: 4.3245

>NTDB_id=54673 RIV7116_RS03325 WP_015116854.1 871257..872366(-) (pilM) [Rivularia sp. PCC 7116]
MVKGLSSLFGKSKSGVGIELAPQRVNVAQLRKQRQGLKLDVLTSVPVPEGIFIDGQIADPPTMAQIIQEALLESKIKASR
VVTAVPGRESIVRLIPVPAELDDKELREMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVQVLLVATRKDVTDTY
ISTFEQAGLQVDVLEISSFSLIRTIRDQLRQFGPQEAAVLVNIEYDSTEIAIVVNGVPQFSRTVPIGTEQMQAALSRAMN
LPTSRDMELLEGMTIPASSMEGGKTGVTEVNPGMAAMLRVLGELTDELRRSVDFYLNQAENLEIAQILLAGSGGGLIQLD
EFFTQRLSLPTTQIDPVQALSLQVDDEDYPFVQRPGLGTVLGLGMRGAP

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=54673 RIV7116_RS03325 WP_015116854.1 871257..872366(-) (pilM) [Rivularia sp. PCC 7116]
GTGGTGAAAGGCTTAAGCAGTCTGTTTGGGAAATCCAAAAGCGGGGTTGGCATCGAGCTTGCACCACAACGAGTGAATGT
AGCTCAGTTACGCAAGCAGCGCCAAGGTTTGAAATTGGATGTTTTAACTTCAGTGCCGGTTCCAGAAGGCATCTTCATTG
ATGGTCAAATAGCAGATCCGCCTACTATGGCACAAATCATCCAAGAGGCGCTTTTGGAAAGCAAAATCAAAGCGAGTCGT
GTTGTAACTGCGGTACCCGGACGCGAGTCTATCGTGCGTCTTATTCCCGTACCTGCAGAATTAGACGATAAAGAGTTACG
GGAAATGGTTCTAAATCATGAAGCAGGTTTGTATCTGCCTTATCCCCGTGAAGAGGCGGATGTGGATTATCAAAAGTTGG
GATACTTTGTAGATGAGGATGGCATCGAAAAGGTACAAGTGCTTTTAGTCGCAACCCGTAAAGATGTTACAGATACCTAC
ATAAGTACTTTTGAACAGGCAGGATTGCAGGTTGATGTTTTAGAAATTAGTAGTTTTTCTCTGATTCGGACAATCCGCGA
CCAACTACGCCAGTTTGGACCGCAGGAAGCCGCAGTCCTAGTAAATATTGAGTACGACAGCACTGAAATTGCAATCGTTG
TTAATGGAGTTCCACAGTTTTCTCGTACCGTTCCTATTGGAACAGAGCAAATGCAAGCAGCCTTATCTAGAGCCATGAAT
TTACCTACATCTAGAGACATGGAACTATTAGAAGGAATGACTATTCCAGCTTCAAGTATGGAAGGCGGCAAAACAGGTGT
AACTGAGGTAAATCCCGGTATGGCTGCCATGTTGAGAGTGTTAGGAGAACTTACCGATGAGCTGCGTCGTTCGGTCGATT
TCTATTTGAATCAGGCTGAGAACTTAGAGATCGCGCAGATATTACTTGCAGGTTCTGGAGGTGGACTTATACAGCTAGAT
GAGTTTTTTACTCAACGCTTGAGTTTACCTACTACTCAGATAGACCCCGTACAAGCTCTATCCCTACAAGTAGATGATGA
AGATTACCCCTTTGTACAGCGTCCTGGTTTGGGTACGGTACTTGGGTTAGGAATGCGAGGTGCTCCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(17-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB K9R730

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

66.848

99.729

0.667

Multiple sequence alignment