Detailed information NTG0544043

Bioinformatically predicted

Overview

• Name: pilB/pilB1
• Type: Machinery gene
• Locus tag: LMF34_RS13770
• Genome accession: NZ_CP086678
• Coordinates: 2882179..2883672 (+)
• Length: 497 a.a.
• NCBI ID: WP_000249375.1
• Uniprot ID: -
• Organism: Escherichia coli strain EFF60
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2877179..2888672

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LMF34_RS13750	pilD	2877827..2878636 (+)	810	WP_000464637.1	prepilin peptidase PppA	Machinery gene
LMF34_RS13755	gspS2	2878702..2879112 (+)	411	WP_001324279.1	type II secretion system pilot lipoprotein GspS-beta	-
LMF34_RS13760	gspC	2879130..2880089 (+)	960	WP_115914941.1	type II secretion system protein GspC	-
LMF34_RS13765	gspD	2880119..2882179 (+)	2061	WP_000498848.1	type II secretion system secretin GspD	-
LMF34_RS13770	pilB/pilB1	2882179..2883672 (+)	1494	WP_000249375.1	type II secretion system ATPase GspE	Machinery gene
LMF34_RS13775	gspF	2883672..2884895 (+)	1224	WP_248422641.1	type II secretion system inner membrane protein GspF	-
LMF34_RS13780	gspG	2884912..2885367 (+)	456	WP_001087296.1	type II secretion system major pseudopilin GspG	-
LMF34_RS13785	gspH	2885371..2885934 (+)	564	WP_248422640.1	type II secretion system minor pseudopilin GspH	-
LMF34_RS13790	gspI	2885931..2886302 (+)	372	WP_115747502.1	type II secretion system minor pseudopilin GspI	-
LMF34_RS13795	gspJ	2886299..2886904 (+)	606	WP_001254778.1	type II secretion system minor pseudopilin GspJ	-
LMF34_RS13800	gspK	2886901..2887878 (+)	978	WP_000633203.1	type II secretion system minor pseudopilin GspK	-

Sequence

Protein

• Length: 497 a.a.
• Molecular weight: 55170.29 Da
• Isoelectric Point: 5.9081

>NTDB_id=544043 LMF34_RS13770 WP_000249375.1 2882179..2883672(+) (pilB/pilB1) [Escherichia coli strain EFF60]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide

• Length: 1494 bp

>NTDB_id=544043 LMF34_RS13770 WP_000249375.1 2882179..2883672(+) (pilB/pilB1) [Escherichia coli strain EFF60]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGCTGTCCCGCTTG
TAATCATAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (123-389)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB/pilB1	Synechocystis sp. PCC 6803	39.556	100	0.431
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.529	85.513	0.406
pilB	Legionella pneumophila strain ERS1305867	42.398	93.964	0.398
pilB	Vibrio parahaemolyticus RIMD 2210633	48.111	79.879	0.384
pilB	Vibrio campbellii strain DS40M4	47.222	79.678	0.376
pilB	Acinetobacter baumannii D1279779	46.366	80.282	0.372
pilB	Vibrio cholerae strain A1552	45.96	79.678	0.366
pilB	Acinetobacter baylyi ADP1	46.41	78.471	0.364
pilF	Thermus thermophilus HB27	45	80.483	0.362