Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   THEOS_RS14250 Genome accession   NC_019386
Coordinates   391617..393065 (-) Length   482 a.a.
NCBI ID   WP_420802238.1    Uniprot ID   -
Organism   Thermus oshimai JL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 386617..398065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEOS_RS02140 (Theos_0429) - 386729..387088 (+) 360 WP_016328699.1 DUF4870 domain-containing protein -
  THEOS_RS02145 (Theos_0430) - 387069..387440 (-) 372 WP_016328700.1 4'-phosphopantetheinyl transferase superfamily protein -
  THEOS_RS02150 (Theos_0431) rsmF 387475..388839 (+) 1365 WP_016328701.1 16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF -
  THEOS_RS02155 (Theos_0432) - 388834..389859 (-) 1026 WP_016328702.1 3-dehydroquinate synthase -
  THEOS_RS02160 (Theos_0433) - 389837..390391 (-) 555 WP_016328703.1 shikimate kinase -
  THEOS_RS02165 (Theos_0434) aroC 390391..391542 (-) 1152 WP_016328704.1 chorismate synthase -
  THEOS_RS14250 pilQ 391617..393065 (-) 1449 WP_420802238.1 secretin N-terminal domain-containing protein Machinery gene
  THEOS_RS14255 - 393046..393120 (+) 75 WP_413217667.1 hypothetical protein -
  THEOS_RS02175 (Theos_0436) pilW 393878..394828 (-) 951 WP_016328706.1 hypothetical protein Machinery gene
  THEOS_RS02180 (Theos_0437) pilO 394825..395388 (-) 564 WP_187288203.1 type 4a pilus biogenesis protein PilO Machinery gene
  THEOS_RS02185 (Theos_0438) pilN 395429..396055 (-) 627 WP_016328708.1 hypothetical protein Machinery gene
  THEOS_RS02190 (Theos_0439) pilM 396048..397193 (-) 1146 WP_016328709.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 482 a.a.        Molecular weight: 51853.53 Da        Isoelectric Point: 5.1418

>NTDB_id=54372 THEOS_RS14250 WP_420802238.1 391617..393065(-) (pilQ) [Thermus oshimai JL-2]
MPGAQITPLPTDPKRAVVLATEADHAKLAGLLQAADLPKAVRRVYTLQNTTLEAVGERLRALLSKELPAARLEAIPGNPK
ALLLEAPESDQALFAELLKAVDVAEAPPPPPQEATVRRLFPLRFADAEKVAPFLAREVPGVVVQTVPGQRILSVRGTEAA
LKEVEALLLQIDRAPEQGPALFQRTYQLSNAKAKDLAAVLQEALKAKTGPQAQGQEATATPTATVVADERTNTLIVTGTA
EDLALVEGLIPRLDQAVPQVNLRVRIQEVQSNFSRALGLKWNTIAGGNVAASIVDAGLSLIFDSTRSLAALNIMATLDAL
QRQGLSRALRDVNQTVLNNQTARLQSGETFLIRRVVGDRVERVPFDIGIIVEVTPQITADGQILLNIKAEVSGNVQRNPV
DGDVDRFTKQVVTTTLRVRDGQTVVLGGLTSQENNETVQGVPFLMDIPLIGELFKQRTGETTDRELLVVITADVLKETAS
RP

Nucleotide


Download         Length: 1449 bp        

>NTDB_id=54372 THEOS_RS14250 WP_420802238.1 391617..393065(-) (pilQ) [Thermus oshimai JL-2]
GTGCCAGGGGCCCAGATCACCCCCCTCCCCACCGACCCCAAGCGGGCCGTGGTCCTGGCCACGGAAGCCGACCACGCCAA
GCTCGCCGGGCTCCTCCAGGCCGCCGACCTGCCCAAGGCGGTCCGCCGGGTCTACACCCTGCAGAACACCACCCTCGAGG
CCGTAGGGGAAAGGCTAAGGGCCCTCCTCTCCAAGGAGCTTCCGGCCGCCCGCCTGGAAGCCATCCCCGGCAACCCCAAA
GCCCTCCTCCTGGAAGCCCCCGAAAGCGACCAGGCCCTCTTCGCCGAACTCCTCAAAGCCGTGGACGTGGCCGAGGCCCC
GCCCCCACCTCCCCAGGAGGCCACGGTGCGCAGGCTCTTCCCCCTGCGCTTCGCCGACGCGGAGAAGGTGGCCCCCTTCC
TGGCCCGGGAGGTGCCGGGCGTGGTGGTCCAGACCGTGCCCGGGCAGCGGATCCTCTCCGTGCGGGGCACGGAAGCGGCC
CTAAAGGAGGTGGAGGCCCTCCTTTTGCAGATTGACCGGGCCCCGGAGCAAGGCCCCGCTCTCTTCCAGCGCACCTACCA
GCTCTCCAACGCCAAGGCCAAGGACTTGGCCGCCGTTCTGCAGGAGGCCCTGAAGGCCAAGACCGGCCCCCAGGCCCAAG
GCCAGGAGGCCACGGCCACCCCCACCGCCACCGTGGTGGCCGACGAGCGCACCAACACCCTCATCGTCACGGGGACCGCG
GAGGACCTGGCCCTGGTGGAAGGCCTCATCCCCCGGCTGGACCAGGCGGTGCCCCAGGTGAACCTGAGGGTGCGCATCCA
GGAGGTGCAGAGCAACTTCAGCCGCGCCCTGGGCCTCAAGTGGAACACCATCGCCGGGGGAAACGTGGCCGCCAGCATCG
TGGACGCCGGGCTTTCCCTCATCTTTGACTCCACCAGGAGCCTCGCCGCCCTCAACATCATGGCCACCCTGGACGCCCTG
CAGCGGCAGGGGCTTTCCCGGGCCCTAAGGGACGTGAACCAGACGGTGCTCAACAACCAGACCGCCCGCCTCCAGTCCGG
GGAGACCTTCCTCATCCGCCGGGTGGTGGGGGACCGGGTGGAGCGGGTGCCCTTTGACATCGGCATCATCGTGGAGGTCA
CGCCCCAGATCACCGCGGACGGCCAGATCCTCCTCAACATCAAGGCGGAGGTCTCGGGGAACGTGCAGCGGAACCCCGTG
GACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCCTCAGGGTCCGGGACGGCCAGACCGTGGTCCTGGG
GGGCCTCACCTCCCAGGAGAACAACGAAACCGTCCAGGGGGTGCCCTTCCTGATGGACATCCCCCTCATCGGGGAGCTCT
TCAAGCAACGGACGGGGGAAACCACCGACCGGGAGCTCCTGGTAGTCATCACCGCGGACGTGCTGAAGGAAACGGCGAGC
AGGCCCTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(118-177)

Bacterial type II/III secretion system short domain (Secretin_N)

(184-259)

Bacterial type II and III secretion system protein (Secretin)

(319-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Thermus thermophilus HB27

79.253

100

0.793

Multiple sequence alignment