Detailed information NTG0054353

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: THEOS_RS02190
• Genome accession: NC_019386
• Coordinates: 396048..397193 (-)
• Length: 381 a.a.
• NCBI ID: WP_016328709.1
• Uniprot ID: -
• Organism: Thermus oshimai JL-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 391048..402193

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
THEOS_RS14250	pilQ	391617..393065 (-)	1449	WP_420802238.1	secretin N-terminal domain-containing protein	Machinery gene
THEOS_RS14255	-	393046..393120 (+)	75	WP_413217667.1	hypothetical protein	-
THEOS_RS02175 (Theos_0436)	pilW	393878..394828 (-)	951	WP_016328706.1	hypothetical protein	Machinery gene
THEOS_RS02180 (Theos_0437)	pilO	394825..395388 (-)	564	WP_187288203.1	type 4a pilus biogenesis protein PilO	Machinery gene
THEOS_RS02185 (Theos_0438)	pilN	395429..396055 (-)	627	WP_016328708.1	hypothetical protein	Machinery gene
THEOS_RS02190 (Theos_0439)	pilM	396048..397193 (-)	1146	WP_016328709.1	type IV pilus assembly protein PilM	Machinery gene
THEOS_RS02195 (Theos_0440)	-	397378..398382 (-)	1005	WP_016328710.1	homoisocitrate dehydrogenase	-
THEOS_RS02200 (Theos_0441)	-	398428..398934 (-)	507	WP_016328711.1	DUF5317 domain-containing protein	-
THEOS_RS02205 (Theos_0442)	-	398970..400373 (-)	1404	WP_041436069.1	HD-GYP domain-containing protein	-
THEOS_RS02210 (Theos_0443)	-	400376..401467 (-)	1092	WP_014514485.1	IS4 family transposase	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 41756.32 Da
• Isoelectric Point: 4.4801

>NTDB_id=54353 THEOS_RS02190 WP_016328709.1 396048..397193(-) (pilM) [Thermus oshimai JL-2]
MVQRLFRQGALSRLFKPRVEALGLEIGAAHLKLVELSGNPPALKAWAMRPTPPGTLVDGLIAEPSALAQELRELLAEART
KKRYVVTAAPNLSVILRTVQVPKMPAKEMEEAVRWEAERYIPFPIDEVVLDYAPLTPLEEAEEGEQVEVMVGAARKESVA
GLIEALRGAGLIPVVVDVKPFAGLYPLEEKLTEGPVTLSVDIGAESTSLVLTQGDRPLYVRILTLSGKDFTEAIGKAFGL
DLISAEEVKRTYGLATIPTEDEELLLDFDAERERYSPARIYDAIRPVLVELTQEIRRSLEFFRVQMGDLQPEVGYLTGGG
SKLRGLPTLLTDTLGVSFEVPNPWEGIAVDPKRFDLEELKGLAPEFMVPVGLALRGVSPLD

Nucleotide

• Length: 1146 bp

>NTDB_id=54353 THEOS_RS02190 WP_016328709.1 396048..397193(-) (pilM) [Thermus oshimai JL-2]
GTGGTTCAAAGGCTCTTTCGTCAGGGCGCACTGAGCCGTCTATTCAAACCCCGGGTTGAGGCCCTGGGCCTGGAGATCGG
GGCGGCGCACCTCAAGCTGGTGGAGCTTTCCGGCAACCCCCCGGCCCTCAAGGCTTGGGCCATGCGGCCCACCCCCCCGG
GCACCCTCGTGGACGGGCTCATCGCCGAGCCCAGCGCCTTAGCTCAAGAACTCCGGGAGCTCCTCGCCGAGGCCAGGACC
AAGAAGCGCTACGTGGTCACCGCGGCCCCCAACCTCTCGGTCATCCTGCGCACGGTCCAGGTGCCCAAGATGCCCGCCAA
GGAGATGGAGGAGGCGGTGCGCTGGGAGGCGGAGCGCTACATCCCCTTCCCCATAGACGAGGTAGTCCTGGACTACGCCC
CCCTCACCCCCCTGGAGGAGGCCGAGGAGGGGGAGCAGGTGGAGGTCATGGTGGGGGCCGCCCGGAAGGAGAGCGTGGCG
GGGCTCATCGAGGCCCTGAGGGGGGCAGGGCTCATCCCGGTGGTGGTGGACGTGAAGCCCTTCGCGGGGCTCTACCCCTT
GGAGGAGAAGCTCACGGAGGGTCCGGTGACCCTTTCCGTGGACATCGGAGCGGAGAGCACCAGCCTGGTCCTCACCCAGG
GGGACCGGCCCCTTTACGTGCGCATCCTCACCCTCTCCGGCAAGGACTTCACCGAGGCCATCGGCAAGGCCTTCGGCCTG
GACCTCATCAGCGCGGAGGAGGTCAAGCGCACCTATGGCCTGGCCACCATCCCCACGGAGGACGAAGAACTCCTCTTGGA
CTTTGACGCGGAGAGGGAACGGTACAGCCCGGCCCGCATCTATGACGCCATCCGCCCCGTGCTGGTGGAGCTCACCCAGG
AGATCCGGCGGAGCCTGGAGTTCTTCCGGGTGCAGATGGGGGACCTCCAGCCCGAGGTGGGCTACCTCACGGGGGGCGGG
AGCAAGCTCCGGGGCCTCCCCACCCTCCTCACGGACACCCTGGGGGTGAGCTTTGAGGTGCCCAACCCCTGGGAGGGCAT
CGCCGTGGACCCCAAGCGCTTTGACCTGGAAGAGCTCAAGGGCCTGGCCCCGGAGTTCATGGTGCCCGTGGGCCTGGCCT
TAAGGGGGGTGAGTCCGCTTGATTAG

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (23-376)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Thermus thermophilus HB27	77.005	98.163	0.756