Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   THEOS_RS02185 Genome accession   NC_019386
Coordinates   395429..396055 (-) Length   208 a.a.
NCBI ID   WP_016328708.1    Uniprot ID   -
Organism   Thermus oshimai JL-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390429..401055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THEOS_RS14250 pilQ 391617..393065 (-) 1449 WP_420802238.1 secretin N-terminal domain-containing protein Machinery gene
  THEOS_RS14255 - 393046..393120 (+) 75 WP_413217667.1 hypothetical protein -
  THEOS_RS02175 (Theos_0436) pilW 393878..394828 (-) 951 WP_016328706.1 hypothetical protein Machinery gene
  THEOS_RS02180 (Theos_0437) pilO 394825..395388 (-) 564 WP_187288203.1 type 4a pilus biogenesis protein PilO Machinery gene
  THEOS_RS02185 (Theos_0438) pilN 395429..396055 (-) 627 WP_016328708.1 hypothetical protein Machinery gene
  THEOS_RS02190 (Theos_0439) pilM 396048..397193 (-) 1146 WP_016328709.1 type IV pilus assembly protein PilM Machinery gene
  THEOS_RS02195 (Theos_0440) - 397378..398382 (-) 1005 WP_016328710.1 homoisocitrate dehydrogenase -
  THEOS_RS02200 (Theos_0441) - 398428..398934 (-) 507 WP_016328711.1 DUF5317 domain-containing protein -
  THEOS_RS02205 (Theos_0442) - 398970..400373 (-) 1404 WP_041436069.1 HD-GYP domain-containing protein -

Sequence

Protein


Download         Length: 208 a.a.        Molecular weight: 23401.93 Da        Isoelectric Point: 10.8019

>NTDB_id=54352 THEOS_RS02185 WP_016328708.1 395429..396055(-) (pilN) [Thermus oshimai JL-2]
MIRLNLLPKDLRRRVEPGWWRLAASLLALFVLLTMGFLHYTALTELNLARSERDALKAEVEALRPFIAEQNRLQRERKTL
EALLAIREGLRKGFVPWSEYLAAFIGQIPRTGGRFPVALRSVNTRALSEEEAKQAAQNGAYDGKTVRVEFTVQGEALNRE
ALVGFVRAFESSPRFGIEFQGATLDRDRGLWTFSARIGAMGGERSAQP

Nucleotide


Download         Length: 627 bp        

>NTDB_id=54352 THEOS_RS02185 WP_016328708.1 395429..396055(-) (pilN) [Thermus oshimai JL-2]
TTGATTAGGCTGAACCTCCTCCCCAAGGACCTTAGGCGCCGGGTGGAGCCCGGCTGGTGGCGGCTTGCGGCCTCCCTCCT
CGCCCTCTTCGTCCTCCTCACCATGGGCTTCCTCCACTACACCGCCCTTACGGAGCTCAATCTGGCCCGCTCGGAGCGGG
ATGCCCTGAAGGCCGAGGTGGAGGCCCTAAGGCCCTTCATCGCCGAGCAGAACCGGCTCCAGCGGGAAAGGAAGACCCTC
GAGGCCCTCCTCGCCATCCGGGAGGGCCTGAGGAAGGGGTTCGTGCCCTGGTCGGAGTACCTGGCGGCCTTCATCGGGCA
GATCCCGCGCACAGGAGGCCGGTTCCCCGTGGCCCTCCGCTCGGTGAACACCCGCGCCCTCTCGGAAGAGGAGGCCAAGC
AGGCCGCCCAGAACGGGGCCTACGACGGCAAGACGGTGCGGGTGGAGTTCACCGTCCAGGGGGAGGCCCTGAACCGGGAA
GCCCTGGTGGGCTTCGTCCGGGCCTTCGAGTCCTCCCCCCGGTTCGGGATCGAGTTCCAAGGGGCCACGCTGGATCGGGA
CCGCGGGCTTTGGACCTTTTCCGCCCGCATAGGGGCCATGGGAGGTGAGCGCAGTGCTCAACCTTAG

Domains

Predicted by InterproScan.

PilN biogenesis protein dimerization domain (PilN_bio_d)

(97-200)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Thermus thermophilus HB27

74.879

99.519

0.745

Multiple sequence alignment