Detailed information NTG0001027

Experimentally validated

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: TT_RS05145
• Genome accession: NC_005835
• Coordinates: 994961..997234 (+)
• Length: 757 a.a.
• NCBI ID: WP_011173436.1
• Uniprot ID: -
• Organism: Thermus thermophilus HB27
• Function: assembly of type IV pilus DNA binding and uptake

Function

PilQ forms macromolecular complexes which consist of 13 protomers forming a stable cone and a cup structure and six stacked rings (N0–N5) with a large central channel. The PilQ complex is highly dynamic and undergoes major conformational changes by opening and closing of two periplasmic gates, thereby making way for pilus extrusion.

Genomic Context

Location: 989961..1002234

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
TT_RS05115 (TT_C1011)	-	990085..990576 (+)	492	WP_011173430.1	DUF5317 domain-containing protein	-
TT_RS05120 (TT_C1012)	-	990630..991634 (+)	1005	WP_011173431.1	homoisocitrate dehydrogenase	-
TT_RS05125 (TT_C1013)	pilM	991769..992902 (+)	1134	WP_011173432.1	type IV pilus assembly protein PilM	Machinery gene
TT_RS05130 (TT_C1014)	pilN	992895..993518 (+)	624	WP_011173433.1	competence protein	Machinery gene
TT_RS05135 (TT_C1015)	pilO	993508..994089 (+)	582	WP_011173434.1	type 4a pilus biogenesis protein PilO	Machinery gene
TT_RS05140 (TT_C1016)	pilW	994086..994964 (+)	879	WP_011173435.1	hypothetical protein	Machinery gene
TT_RS05145 (TT_C1017)	pilQ	994961..997234 (+)	2274	WP_011173436.1	secretin N-terminal domain-containing protein	Machinery gene
TT_RS05150 (TT_C1018)	aroC	997293..998444 (+)	1152	WP_011173437.1	chorismate synthase	-
TT_RS05155 (TT_C1019)	-	998469..999023 (+)	555	WP_011173438.1	shikimate kinase	-
TT_RS05160 (TT_C1020)	-	999001..1000047 (+)	1047	WP_011173439.1	3-dehydroquinate synthase	-
TT_RS05165 (TT_C1021)	rsmF	1000093..1001463 (-)	1371	WP_041443492.1	16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF	-
TT_RS05170 (TT_C1022)	-	1001516..1001893 (+)	378	WP_011173441.1	4'-phosphopantetheinyl transferase superfamily protein	-
TT_RS05175 (TT_C1023)	-	1001871..1002224 (-)	354	WP_011173442.1	DUF4870 domain-containing protein	-

Sequence

Protein

• Length: 757 a.a.
• Molecular weight: 82637.62 Da
• Isoelectric Point: 5.1292

>NTDB_id=1027 TT_RS05145 WP_011173436.1 994961..997234(+) (pilQ) [Thermus thermophilus HB27]
MKSAWIRAAVIALAGLGAFALAGSFPEEPRFQAPVNLKVSESQVKAGQTLPLDVVLEALARSVGLQPLIYRAYDPSGDPA
TAQPPLPNVKLDFQGKPFREVWDLLFATYGNQYSLDYLFLPPDVVVVAPTQVITALVDAPSRTGAMERRPYIVGVPEIAY
KRTETDAQGQPRTVVNIEGAKAWVQNDLLPFLSREAAGLNVNWIVVEEGGRLKAVLSVLATPEQHARFSDILQRAGIDFR
PLPALAQPKPRVEKTYTLTYATFPDLLAFLQSRLPEAQIGVVPTNPQRAIVLATEEDHARLSELLKTADVPKTVRRVYTL
QNLTFAEAQERLKPLLEKDLKGARLESLPGNPKALLLEAPEAEHALFAEILKALDVPPQAPQAPQEATLRRLYPLHYANA
EQVAPFLAREVPGIVVQTVPGQPLLSVRGTEAQLREVESLLAQIDRPPEQGPPVFQRAYQLSNAKAVELAQVLQEALKAR
QAQNQGQQNQAPPTREATVVADPRTNTLIVTGTQEDLALVEGLIPKLDQPVPQVNLRVRIQEVQSNLTRSLGLKWNSIAG
GNVAASILDSGLSLIFDSTRSLAALNIMATLDALQQQGLSRALRDVNQTVLNNQTARLQSGETFFIRRVVNDQVERVPFD
VGLIVEVTPQITADGQILLNIKAEVSGNVQRNPVDGDVDRFTKQVVTTTLRVKDGETVVLGGLTSQESNQSQQGVPLLMD
IPLIGELFKQRTNESTDKELLVVITADILKEAASANP

Nucleotide

• Length: 2274 bp

>NTDB_id=1027 TT_RS05145 WP_011173436.1 994961..997234(+) (pilQ) [Thermus thermophilus HB27]
ATGAAGAGCGCGTGGATCCGTGCGGCCGTGATCGCCCTCGCAGGGCTAGGGGCTTTTGCCCTTGCGGGGAGCTTTCCCGA
GGAGCCCCGCTTCCAGGCCCCGGTGAACCTCAAGGTCTCGGAATCCCAGGTCAAGGCGGGCCAGACCCTGCCCTTGGACG
TGGTCCTCGAGGCCCTGGCGCGAAGCGTGGGGCTCCAGCCCCTCATCTACCGGGCCTACGACCCGAGCGGGGACCCCGCC
ACGGCCCAGCCCCCCCTGCCCAACGTCAAGCTGGACTTCCAGGGCAAGCCCTTCCGGGAGGTCTGGGACCTCCTCTTCGC
CACCTACGGCAACCAGTACAGCCTGGACTACCTCTTCCTGCCCCCCGACGTGGTGGTGGTGGCCCCCACCCAGGTGATCA
CCGCCCTGGTGGACGCCCCGAGCCGCACGGGGGCCATGGAGCGCAGGCCCTACATCGTGGGCGTCCCCGAGATCGCCTAC
AAGCGCACGGAGACGGACGCCCAGGGCCAGCCCCGCACGGTCGTCAACATCGAGGGCGCCAAGGCCTGGGTCCAGAACGA
TCTCCTCCCCTTCCTCTCCCGGGAGGCGGCGGGGCTCAACGTGAACTGGATCGTGGTGGAGGAAGGGGGTAGGCTCAAGG
CCGTCCTCTCCGTCCTCGCCACCCCCGAGCAGCACGCCCGCTTCTCCGATATCCTGCAGCGGGCCGGGATTGACTTCCGC
CCCCTCCCCGCCCTGGCCCAGCCTAAGCCCCGGGTGGAGAAGACCTACACCCTCACCTACGCCACCTTCCCCGACCTCCT
CGCCTTCCTCCAGTCCCGTCTCCCCGAGGCCCAGATCGGCGTGGTCCCCACCAACCCCCAAAGGGCCATCGTCCTCGCCA
CCGAGGAGGACCACGCCCGCTTGAGCGAGCTTCTGAAGACGGCCGACGTCCCCAAGACCGTCCGCCGGGTCTACACCCTG
CAGAACCTCACCTTCGCCGAGGCCCAGGAGCGCCTAAAGCCCCTCCTGGAGAAGGACCTCAAGGGGGCCCGCCTGGAAAG
CCTCCCCGGCAACCCCAAGGCCCTCCTCCTCGAGGCCCCCGAGGCTGAGCACGCCCTCTTCGCCGAGATCCTGAAGGCCC
TGGACGTCCCCCCCCAGGCCCCCCAAGCCCCCCAGGAGGCCACCTTGCGGCGCCTCTACCCCCTGCACTACGCCAACGCC
GAGCAGGTGGCCCCCTTCCTCGCCCGGGAGGTGCCGGGGATCGTGGTCCAGACCGTCCCCGGCCAACCCCTCCTCTCGGT
GAGGGGCACGGAAGCGCAGCTTAGGGAGGTGGAAAGCCTCCTCGCCCAGATTGACCGCCCCCCGGAGCAGGGCCCTCCCG
TGTTCCAGCGGGCCTACCAGCTCTCCAACGCCAAGGCGGTGGAGCTCGCCCAGGTGCTCCAGGAGGCGCTCAAGGCCCGG
CAGGCCCAGAACCAGGGCCAGCAGAACCAGGCCCCCCCCACCCGGGAGGCCACGGTGGTGGCCGACCCCAGGACCAACAC
CCTCATCGTCACCGGCACCCAGGAGGACCTCGCCCTGGTGGAGGGCCTCATCCCCAAGCTGGACCAGCCCGTGCCCCAGG
TGAACCTCAGGGTGCGCATCCAGGAGGTCCAGTCCAACCTGACCCGCAGCCTCGGCCTCAAGTGGAACAGCATCGCCGGC
GGCAACGTGGCGGCCAGCATCCTGGACTCGGGCCTTTCCCTCATCTTTGACTCCACGCGAAGCCTCGCCGCCCTCAACAT
CATGGCCACCCTGGACGCCCTGCAGCAACAGGGCCTCTCCCGGGCCCTCCGGGACGTGAACCAGACCGTCCTCAACAACC
AAACCGCCCGCCTGCAGTCCGGCGAGACCTTCTTCATCCGGCGGGTGGTGAACGACCAGGTGGAACGGGTCCCCTTTGAC
GTGGGGCTCATCGTGGAGGTCACGCCCCAGATCACCGCCGACGGCCAGATCCTCCTCAACATCAAGGCCGAGGTCTCGGG
GAACGTGCAGCGCAACCCCGTGGACGGGGACGTGGACCGCTTCACCAAGCAGGTGGTGACCACCACCCTCAGGGTCAAAG
ACGGGGAGACCGTGGTCCTCGGGGGCCTCACCTCCCAGGAGTCCAACCAGAGCCAGCAGGGGGTGCCCCTCCTCATGGAC
ATCCCCCTGATCGGCGAGCTCTTCAAGCAGCGGACGAACGAGAGCACCGACAAGGAGCTTTTGGTGGTGATCACCGCCGA
CATCCTCAAGGAGGCGGCCTCGGCGAACCCCTAA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (593-750)

• Bacterial type II/III secretion system short domain (Secretin_N) (391-450)

• Bacterial type II/III secretion system short domain (Secretin_N) (457-533)

References

• [1]: Deniz Yaman et al. (2024) Identification of subcomplexes and protein-protein interactions in the DNA transporter of Thermus thermophilus HB27. Biochimica Et Biophysica Acta. Biomembranes 1866(7):184363. [PMID: 38909880]
• [2]: Beate Averhoff et al. (2021) Natural transformation in Gram-negative bacteria thriving in extreme environments: from genes and genomes to proteins, structures and regulation. Extremophiles : Life Under Extreme Conditions 25(5-6):425-436. [PMID: 34542714]
• [3]: Edoardo D'Imprima et al. (2017) Cryo-EM structure of the bifunctional secretin complex of Thermus thermophilus. ELife 6:e30483. [PMID: 29280731]
• [4]: Ralf Salzer et al. (2016) Topology and Structure/Function Correlation of Ring- and Gate-forming Domains in the Dynamic Secretin Complex of Thermus thermophilus. The Journal of Biological Chemistry 291(28):14448-56. [PMID: 27226590]
• [5]: Vicki A M Gold et al. (2015) Structure of a type IV pilus machinery in the open and closed state. ELife 4:e07380. [PMID: 25997099]
• [6]: Janin Burkhardt et al. (2012) Unusual N-terminal ααβαββα fold of PilQ from Thermus thermophilus mediates ring formation and is essential for piliation. The Journal of Biological Chemistry 287(11):8484-94. [PMID: 22253437]
• [7]: Janin Burkhardt et al. (2011) Structure and function of PilQ, a secretin of the DNA transporter from the thermophilic bacterium Thermus thermophilus HB27. The Journal of Biological Chemistry 286(12):9977-84. [PMID: 21285351]
• [8]: Cornelia Schwarzenlander et al. (2009) The role of single subunits of the DNA transport machinery of Thermus thermophilus HB27 in DNA binding and transport. Environmental Microbiology 11(4):801-8. [PMID: 19396940]
• [9]: Judit Rumszauer et al. (2006) Identification, subcellular localization and functional interactions of PilMNOWQ and PilA4 involved in transformation competency and pilus biogenesis in the thermophilic bacterium Thermus thermophilus HB27. The FEBS Journal 273(14):3261-72. [PMID: 16857013]
• [10]: Alexandra Friedrich et al. (2002) Molecular analyses of the natural transformation machinery and identification of pilus structures in the extremely thermophilic bacterium Thermus thermophilus strain HB27. Applied And Environmental Microbiology 68(2):745-55. [PMID: 11823215]