Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LMH79_RS22760 Genome accession   NZ_CP086259
Coordinates   4641472..4642965 (-) Length   497 a.a.
NCBI ID   WP_000249325.1    Uniprot ID   -
Organism   Escherichia coli strain SCPM-O-B-9427     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4636472..4647965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LMH79_RS22730 (LMH79_22755) gspK 4637281..4638243 (-) 963 WP_000631645.1 type II secretion system minor pseudopilin GspK -
  LMH79_RS22735 (LMH79_22760) gspJ 4638240..4638845 (-) 606 WP_001364472.1 type II secretion system minor pseudopilin GspJ -
  LMH79_RS22740 (LMH79_22765) gspI 4638842..4639213 (-) 372 WP_000820133.1 type II secretion system minor pseudopilin GspI -
  LMH79_RS22745 (LMH79_22770) gspH 4639210..4639773 (-) 564 WP_001115109.1 type II secretion system minor pseudopilin GspH -
  LMH79_RS22750 (LMH79_22775) gspG 4639777..4640232 (-) 456 WP_001087297.1 type II secretion system major pseudopilin GspG -
  LMH79_RS22755 (LMH79_22780) gspF 4640249..4641472 (-) 1224 WP_001173420.1 type II secretion system inner membrane protein GspF -
  LMH79_RS22760 (LMH79_22785) pilB/pilB1 4641472..4642965 (-) 1494 WP_000249325.1 type II secretion system ATPase GspE Machinery gene
  LMH79_RS22765 (LMH79_22790) gspD 4642965..4645022 (-) 2058 WP_000942636.1 type II secretion system secretin GspD -
  LMH79_RS22770 (LMH79_22795) gspC 4645082..4645945 (-) 864 WP_000277223.1 type II secretion system protein GspC -
  LMH79_RS22775 (LMH79_22800) gspS2 4646046..4646456 (-) 411 WP_000890768.1 type II secretion system pilot lipoprotein GspS-beta -
  LMH79_RS22780 (LMH79_22805) pilD 4646497..4647255 (-) 759 WP_000522992.1 prepilin peptidase Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55262.44 Da        Isoelectric Point: 6.1287

>NTDB_id=541641 LMH79_RS22760 WP_000249325.1 4641472..4642965(-) (pilB/pilB1) [Escherichia coli strain SCPM-O-B-9427]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKKQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGTITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCTDCRQPAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=541641 LMH79_RS22760 WP_000249325.1 4641472..4642965(-) (pilB/pilB1) [Escherichia coli strain SCPM-O-B-9427]
ATGGTGCCTGTAGCACAGGAAACCACCGCCAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGACTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGATTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGATGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCACTGCTGGTATCGCGTATTAAGGTGATGGCGCGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGAATTGCGCTGCTGCTGGGTGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAAACAGGCCCGCCTGACGCTGGAGCGTCTGGGGTTAAGCCACGAACTGACC
GCACAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCGTCGCTCACTGGGCACCT
GGTACTTTCCACACTGCATACCAACACGGCGGTGGGGACGATTACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCACCGACTGCCGTCAGCCCGCGCCTGCC
ACTGATGAAGAAAAACGCTTGCTGGGAATTACCGACGCGCGTACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACCGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGCATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTGCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.852

86.72

0.38
  pilB Vibrio campbellii strain DS40M4

47.475

79.678

0.378
  pilB Acinetobacter baumannii D1279779

46.867

80.282

0.376
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

44.75

80.483

0.36
  pilB Acinetobacter baylyi ADP1

46.134

78.068

0.36