Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LGV82_RS02755 Genome accession   NZ_CP086104
Coordinates   594388..596526 (+) Length   712 a.a.
NCBI ID   WP_275541829.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RS650     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 589388..601526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGV82_RS02735 (LGV82_02730) pilM 591465..592535 (+) 1071 WP_011002880.1 type IV pilus biogenesis protein PilM -
  LGV82_RS02740 (LGV82_02735) - 592549..593181 (+) 633 WP_011002879.1 PilN domain-containing protein -
  LGV82_RS02745 (LGV82_02740) - 593184..593843 (+) 660 WP_020832821.1 type 4a pilus biogenesis protein PilO -
  LGV82_RS02750 (LGV82_02745) - 593840..594385 (+) 546 WP_011002877.1 pilus assembly protein PilP -
  LGV82_RS02755 (LGV82_02750) pilQ 594388..596526 (+) 2139 WP_275541829.1 temperature dependent type IV pilus secretin PilQ Machinery gene
  LGV82_RS02760 (LGV82_02755) - 596668..597240 (+) 573 WP_011002875.1 shikimate kinase -
  LGV82_RS02765 (LGV82_02760) aroB 597237..598343 (+) 1107 WP_011002874.1 3-dehydroquinate synthase -
  LGV82_RS02770 (LGV82_02765) - 598363..599526 (+) 1164 WP_011002873.1 deoxyguanosinetriphosphate triphosphohydrolase -
  LGV82_RS02775 (LGV82_02770) - 599676..600350 (-) 675 WP_011002872.1 OmpW family protein -
  LGV82_RS02780 (LGV82_02775) - 600617..601342 (+) 726 WP_247318054.1 transposase -

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76379.47 Da        Isoelectric Point: 9.8259

>NTDB_id=540343 LGV82_RS02755 WP_275541829.1 594388..596526(+) (pilQ) [Ralstonia pseudosolanacearum strain RS650]
MRRVKGGLVQAVRVARGGAVAWLSLCMLMVGGQALAQQAITAPASNAVERVEQASTGESTVVTVTLKDTPAQKPVEFSTQ
QPARIAIDFFGTAFAQGRANYQYGGKLLRAASVVQIGDRTRVVLDLARQSQYKSEIRGNQYVLTLGAAPTASATPVPTFA
APAPTAGVERPSVRNIDFRRGEELAGRVVVDLSTSNSAINIAQQGQNLVVDFAGATLPQSLRRRFDVSDFGTPVQAMRAT
DNGTGARLVIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERMSLNFQNIDIRSLLQVFADFTNLNIVTSD
SVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRNELATKEKAELESQQQVTELEPLRSQVFRLNYQRADDV
RNMLLGTGGTAGGGAAASRILSKRGSLTSDARTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEADDTFSRNLG
AKLGFAAKTNGAGYGNTYSNVVSPVTKDATWDNSAAVSLPANGINGVNAASVAVSLFNAGAGRFLALELSALEADGRGKI
ISSPRVVTADNIKALIEQGTEVPYQQATSSGATSVSFKKANLKLEVTPKITPDGNVFLDVDVNKDSLGTQTTNGFAINTK
HVQTQVLVENGGTVVIGGIYTQNERTDVNKVPLLGDIPVLGNLFKSTAKTNDRTELLVFLTPRVLSDQLSLK

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=540343 LGV82_RS02755 WP_275541829.1 594388..596526(+) (pilQ) [Ralstonia pseudosolanacearum strain RS650]
ATGCGTCGCGTGAAGGGCGGCCTGGTGCAGGCCGTTCGAGTGGCCCGGGGGGGCGCAGTGGCATGGCTTTCGCTGTGCAT
GCTGATGGTGGGTGGACAGGCGTTGGCCCAGCAGGCAATCACGGCGCCGGCAAGCAATGCCGTCGAACGCGTGGAACAAG
CCTCGACAGGCGAATCCACCGTGGTGACGGTGACGCTGAAGGACACGCCCGCCCAGAAGCCGGTGGAATTCAGCACGCAG
CAGCCTGCGCGCATCGCCATCGACTTCTTCGGCACGGCATTCGCCCAGGGGCGTGCCAACTATCAATATGGCGGCAAGCT
GCTGCGCGCGGCCAGCGTGGTGCAGATCGGTGACCGCACCCGCGTCGTGCTGGATCTGGCGCGCCAGTCGCAGTACAAGT
CCGAGATCCGGGGCAACCAGTACGTGCTGACGCTGGGCGCCGCGCCGACGGCGTCGGCCACCCCGGTGCCGACCTTCGCC
GCGCCCGCGCCGACGGCCGGTGTGGAGCGGCCGTCCGTGCGCAACATCGACTTCCGCCGTGGCGAAGAGCTGGCCGGCCG
CGTGGTGGTCGACCTGTCGACGTCCAACTCCGCGATCAACATCGCTCAGCAGGGGCAAAACCTGGTGGTCGATTTCGCCG
GTGCCACGCTGCCGCAATCGCTGCGTCGCCGCTTTGACGTGAGCGACTTCGGCACGCCGGTGCAGGCCATGCGCGCCACC
GACAACGGCACGGGCGCTCGCCTGGTCATCGAGCCGCGCGGCAACTGGCAGTACAGCTCGTACCAGACCGACACGCAGTT
TGTGGTGGAAGTCCGCCCGACCAAGGAGGACCCGAACAAGCTGATCTCCGGTCCGGGCTATCGCGGGGAGCGCATGTCGC
TGAACTTCCAGAACATCGACATTCGTTCGCTGCTGCAGGTCTTTGCCGACTTCACCAACCTGAACATCGTCACCAGCGAC
AGCGTGACGGGGACCCTGTCGCTGCGTCTGAAGGACGTGCCGTGGGATCAGGCGCTGCAGATCGTGCTGGATTCCAAGGG
CCTGGCCTCGCGCCGCAACGGCAACGTGCTGTGGGTGGCGCCGCGCAACGAACTGGCGACCAAGGAGAAGGCTGAGCTGG
AGTCGCAGCAACAGGTGACCGAGCTGGAGCCGCTGCGCAGCCAGGTGTTCCGTCTGAATTACCAGCGTGCTGACGACGTG
CGCAACATGCTGCTGGGTACCGGAGGAACGGCGGGCGGTGGTGCTGCGGCGTCGCGCATCTTGTCCAAGCGTGGCTCGCT
GACGTCGGATGCCCGTACCAACCAGCTGTTCGTCTCGGATATCCCGAGCAAGCTGGAGGAGGTGCAGGCCTTCCTGCTGA
AGATCGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGATGACACGTTCAGCCGCAATCTTGGG
GCAAAGCTGGGCTTCGCGGCCAAGACCAACGGTGCCGGGTATGGCAACACCTACAGCAACGTGGTGTCGCCGGTCACGAA
AGACGCTACTTGGGATAACAGCGCGGCCGTCAGCCTGCCCGCCAATGGCATCAACGGTGTGAACGCCGCCAGTGTGGCAG
TGAGCTTGTTCAACGCCGGTGCCGGCCGTTTCCTGGCGCTCGAACTGTCGGCGCTGGAAGCCGATGGCCGCGGCAAGATC
ATCTCCAGCCCGCGTGTGGTCACCGCTGACAACATCAAGGCGCTGATCGAGCAGGGTACCGAAGTGCCTTACCAGCAGGC
GACCTCGTCGGGTGCGACGTCGGTTTCGTTCAAAAAGGCCAACCTGAAATTGGAGGTGACGCCGAAGATTACGCCGGACG
GCAACGTGTTCCTGGACGTGGACGTCAACAAGGACAGCCTGGGCACGCAGACCACCAACGGCTTCGCCATCAACACCAAG
CATGTGCAGACCCAGGTGCTGGTCGAGAACGGCGGTACGGTTGTGATTGGCGGCATCTACACGCAGAACGAACGGACCGA
TGTGAACAAGGTCCCGCTGCTGGGTGACATCCCGGTGCTGGGCAACCTGTTCAAGAGCACGGCCAAGACCAACGACCGGA
CGGAACTGCTGGTGTTCCTGACGCCGCGCGTGCTGTCCGACCAGCTGTCGCTGAAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(551-705)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-461)

Secretin and TonB N terminus short domain (STN)

(316-361)

AMIN domain (AMIN)

(61-143)

AMIN domain (AMIN)

(195-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

41.467

93.82

0.389
  pilQ Neisseria gonorrhoeae strain FA1090

36.401

100

0.372
  comQ/pilQ Acinetobacter baylyi ADP1

38.15

97.191

0.371
  pilQ Legionella pneumophila strain Lp02

39.269

92.275

0.362
  pilQ Legionella pneumophila strain ERS1305867

39.269

92.275

0.362