Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   JWV26_RS13355 Genome accession   NZ_CP070505
Coordinates   2898483..2899547 (+) Length   354 a.a.
NCBI ID   WP_017678539.1    Uniprot ID   A0A1G6YHQ2
Organism   Ectopseudomonas toyotomiensis strain SM2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2893483..2904547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWV26_RS13345 (JWV26_13345) - 2894531..2895799 (+) 1269 WP_017678537.1 malic enzyme-like NAD(P)-binding protein -
  JWV26_RS13350 (JWV26_13350) - 2895890..2898301 (-) 2412 WP_196457609.1 penicillin-binding protein 1A -
  JWV26_RS13355 (JWV26_13355) pilM 2898483..2899547 (+) 1065 WP_017678539.1 pilus assembly protein PilM Machinery gene
  JWV26_RS13360 (JWV26_13360) - 2899547..2900122 (+) 576 WP_206417051.1 PilN domain-containing protein -
  JWV26_RS13365 (JWV26_13365) pilO 2900119..2900745 (+) 627 WP_196457603.1 type 4a pilus biogenesis protein PilO -
  JWV26_RS13370 (JWV26_13370) pilP 2900742..2901266 (+) 525 WP_196457600.1 type 4a pilus biogenesis lipoprotein PilP -
  JWV26_RS13375 (JWV26_13375) pilQ 2901321..2903420 (+) 2100 WP_196457961.1 type IV pilus secretin PilQ Machinery gene
  JWV26_RS13380 (JWV26_13380) aroK 2903426..2903944 (+) 519 WP_206417052.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37829.19 Da        Isoelectric Point: 4.4647

>NTDB_id=540327 JWV26_RS13355 WP_017678539.1 2898483..2899547(+) (pilM) [Ectopseudomonas toyotomiensis strain SM2]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYSVEPLPPNAVVEKNIAELEGVGQALSRVLAKAKSGVKTAAV
AVAGSAVITKTIEMEAGLSEDDLENQLKIEADQYIPYPLEEVAIDFEVQGPAPRNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLEAQLGGGHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=540327 JWV26_RS13355 WP_017678539.1 2898483..2899547(+) (pilM) [Ectopseudomonas toyotomiensis strain SM2]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACTTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAAGTAGAGGCTTATTCAGTCGAGCCGCTTCCACCTAACGCAGTGGTCGAGAAGAACA
TCGCCGAGCTGGAGGGGGTCGGTCAGGCGCTCTCTCGCGTATTGGCCAAGGCCAAGAGCGGCGTCAAGACGGCTGCAGTT
GCTGTGGCGGGTTCGGCTGTCATCACCAAGACCATCGAAATGGAAGCAGGTCTTTCCGAGGACGACTTGGAAAACCAGCT
AAAGATCGAGGCCGATCAGTACATTCCCTACCCGCTGGAAGAAGTCGCCATCGATTTCGAAGTGCAGGGGCCGGCGCCGC
GCAACCCCGAGCGGGTCGAGGTGCTGCTGGCTGCATGCCGTAAGGAGAACGTGGAGGTCCGCGAGGCTGCCCTTGCGCTC
GCAGGACTTACCGCGAAAGTGGTCGATGTCGAGGCGTATGCCCTCGAACGCGCTTACGGTTTGCTGGAGGCGCAACTGGG
CGGCGGTCACGATGAGTTGACCGTCGCGGTGGTGGATATTGGCGCGACGATGACCACGCTCAGTGTTCTGCATAATGGCC
GCACCATCTACACCCGCGAGCAGCTTTTCGGGGGCAAGCAGCTGACCGAGGAAATCCAGCGCCGCTACGGCCTGTCGGTC
GAGGAAGCTGGGCTTGCCAAGAAGCAAGGTGGTCTTCCAGATGACTACGACAGTGAGGTTTTGCAACCGTTCAAAGAAGC
TGTCGTTCAGCAGGTTTCGCGCTCGCTGCAGTTCTTCTTCGCCGCCGGGCAATTCAATGATGTTGACTACATCCTCCTGG
CTGGCGGCACCGCGTCCATTCCTGATCTCGATCGGTTGATCCAGCAGAAGATTGGTACGCAGACGCTAGTGGCCAATCCC
TTTGCCGATATGGCGCTGAGCAGCAAGGTCAACGCCGGTGCGCTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGTGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1G6YHQ2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.39

100

0.534
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449