Detailed information NTG0540312

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: JWU58_RS25385
• Genome accession: NZ_CP070503
• Coordinates: 5605924..5606988 (-)
• Length: 354 a.a.
• NCBI ID: WP_024011266.1
• Uniprot ID: V8RDF4
• Organism: Pseudomonas atacamensis strain SM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5600924..5611988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JWU58_RS25360 (JWU58_25360)	aroK	5601602..5602120 (-)	519	WP_024011270.1	shikimate kinase AroK	-
JWU58_RS25365 (JWU58_25365)	pilQ	5602125..5604203 (-)	2079	WP_206421196.1	type IV pilus secretin PilQ	Machinery gene
JWU58_RS25370 (JWU58_25370)	-	5604217..5604741 (-)	525	WP_016772049.1	pilus assembly protein PilP	-
JWU58_RS25375 (JWU58_25375)	pilO	5604738..5605361 (-)	624	WP_024011267.1	type 4a pilus biogenesis protein PilO	-
JWU58_RS25380 (JWU58_25380)	-	5605358..5605924 (-)	567	WP_016772051.1	PilN domain-containing protein	-
JWU58_RS25385 (JWU58_25385)	pilM	5605924..5606988 (-)	1065	WP_024011266.1	pilus assembly protein PilM	Machinery gene
JWU58_RS25390 (JWU58_25390)	-	5607208..5609652 (+)	2445	WP_420893298.1	penicillin-binding protein 1A	-
JWU58_RS25395 (JWU58_25395)	-	5609851..5611119 (-)	1269	WP_016772053.1	malic enzyme-like NAD(P)-binding protein	-
JWU58_RS25400 (JWU58_25400)	-	5611304..5611987 (-)	684	WP_206422224.1	thermonuclease family protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38045.57 Da
• Isoelectric Point: 4.7539

>NTDB_id=540312 JWU58_RS25385 WP_024011266.1 5605924..5606988(-) (pilM) [Pseudomonas atacamensis strain SM1]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=540312 JWU58_RS25385 WP_024011266.1 5605924..5606988(-) (pilM) [Pseudomonas atacamensis strain SM1]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTGGAGCCGTTGCCGGCGAACGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCGGTG
GCAGTGGCGGGTTCGGCAGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACGCATCTGGC
GGCCTCACAGGAGCGCCTGACAGTGGCGGTGGTCGACATTGGCGCGACCATGACCACTCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTTGGCGGGCGCCAACTCACCGAAGAGATTCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAGCAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCCTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGTTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CGGGCGGCACCGCATCGGTCCCCGGCCTGGACCGCCTGATCGAACAGCGTCTGGACACGCCGACGCAGGTCGCCAACCCG
TTCGCCGACATGGCTTTAGGCAGCAAGGTCAATGCTGGGGCGCTGGCCAGTGATGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: V8RDF4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.215	100	0.562
comM	Acinetobacter nosocomialis M2	56.215	100	0.562
comM	Acinetobacter baylyi ADP1	54.52	100	0.545
pilM	Legionella pneumophila strain ERS1305867	48.305	100	0.483