Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   JWU58_RS25365 Genome accession   NZ_CP070503
Coordinates   5602125..5604203 (-) Length   692 a.a.
NCBI ID   WP_206421196.1    Uniprot ID   -
Organism   Pseudomonas atacamensis strain SM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5597125..5609203
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWU58_RS25350 (JWU58_25350) - 5598752..5600338 (-) 1587 WP_130902918.1 AAA family ATPase -
  JWU58_RS25355 (JWU58_25355) aroB 5600349..5601449 (-) 1101 WP_206421195.1 3-dehydroquinate synthase -
  JWU58_RS25360 (JWU58_25360) aroK 5601602..5602120 (-) 519 WP_024011270.1 shikimate kinase AroK -
  JWU58_RS25365 (JWU58_25365) pilQ 5602125..5604203 (-) 2079 WP_206421196.1 type IV pilus secretin PilQ Machinery gene
  JWU58_RS25370 (JWU58_25370) - 5604217..5604741 (-) 525 WP_016772049.1 pilus assembly protein PilP -
  JWU58_RS25375 (JWU58_25375) pilO 5604738..5605361 (-) 624 WP_024011267.1 type 4a pilus biogenesis protein PilO -
  JWU58_RS25380 (JWU58_25380) - 5605358..5605924 (-) 567 WP_016772051.1 PilN domain-containing protein -
  JWU58_RS25385 (JWU58_25385) pilM 5605924..5606988 (-) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74866.12 Da        Isoelectric Point: 5.4106

>NTDB_id=540311 JWU58_RS25365 WP_206421196.1 5602125..5604203(-) (pilQ) [Pseudomonas atacamensis strain SM1]
MNRIFSTLGFSLWIALVSPMVMAASLKALDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVTSQLASRNLD
LGSGNARTATVAEAKDRTRLIVSLTQLAPYSTRVEGNNLFVVVGQGASATAPRPAAVAARAATAAAAPARPAAQRSRAIR
GVDFQRGTEGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPEKLRVRLDVKDFATPVQFVNAALTGDRTVITVEPSG
TFDYSTFQTDNKLTVSVRPMSIDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNASGVN
GSSSTIGTPGSTGTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=540311 JWU58_RS25365 WP_206421196.1 5602125..5604203(-) (pilQ) [Pseudomonas atacamensis strain SM1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGGTGTCACCGATGGTAATGGCGGCCAGTCTGAA
AGCGCTGGATGTTGCCGCGCTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGACGGCCCGCCACCACAGCCCAAGG
GCTACACAACCGAGTCGCCGGCGCGCATTGCACTGGATCTGCCGGGCGTGACCAGCCAGTTGGCCAGCAGGAATCTTGAT
CTGGGCAGCGGCAACGCGCGCACGGCCACGGTGGCCGAAGCCAAGGATCGCACGCGGCTGATTGTCAGCCTGACGCAGTT
GGCGCCTTACAGCACGCGGGTCGAGGGCAATAATCTGTTTGTGGTGGTCGGTCAGGGCGCTTCCGCGACTGCGCCGCGCC
CGGCTGCCGTGGCAGCGCGTGCCGCCACGGCCGCTGCGGCACCTGCCAGACCAGCCGCGCAACGAAGCAGGGCGATTCGC
GGTGTCGATTTCCAGCGCGGCACCGAGGGTGAGGGCAACGTAGTCATCGACCTCTCCGATCCGACCATCGCCCCGGATAT
CCAGGAGCACGATGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCGGAAAAACTGCGCGTGCGCCTCGACGTCA
AGGACTTCGCCACGCCCGTGCAGTTCGTCAACGCAGCGCTCACCGGCGATCGCACCGTGATCACTGTCGAGCCCAGCGGC
ACCTTCGACTACTCCACGTTTCAGACCGATAACAAGCTCACCGTGAGCGTGCGGCCGATGAGCATCGACGACCTGCAAAA
GCGCAACGCCGACCGTCAGGCCTATGTCGGCGACAAGCTCTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCGGACTTCACCAATCTCAATCTGGTGGCGAGCGATACGGTGCAGGGCGGTATCACTTTGCGCCTGCAGAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGACTGGACAAGCGCAAGATCGGCAACGTGCTGCTGGT
CGCGCCGGCCGATGAAATTGCCGCTCGCGAGCGTCAGGAACTGGAATCGCAGAAGCAGATCGCCGAGCTGGCGCCCCTGC
GTCGTGAGCTGCTGCAAGTCAATTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCCGTGACCAGTGCCGAGGCC
AAGGTCGACGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGATCGCCTCGA
CGAACTCCGGCGGATCGTCGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCGTTGAGGCCAACG
TCGATTATGACAAGAGCCTGGGCGTGCGCTGGGGCGGTTCGGTGCAGAACAAGGGCAACTGGAACGCCTCAGGGGTCAAT
GGCTCCTCGAGCACCATCGGTACGCCGGGCAGCACCGGCACCAACTCGCCGTTCGTCGACATGGGCACGGTCAACAATAC
CTCGGGGATCGGCATTGCTTTCATCACCGACAACGTACTGCTGGATCTCGAACTGACGGCGATGGAAAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAGGTGGTCACCTCGGACAAGGAAACCGCGAAGATCCTCAAAGGCACCGAGATTCCCTAT
CAGGAGGCCAGCTCCAGCGGCGCGACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACTCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAAGTGCTGGTAAATGACGGCGAGACCATCGTAATCGGCGGTGTTTTTTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
TTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.028

100

0.744
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii strain A118

37.787

100

0.405
  pilQ Acinetobacter baumannii D1279779

37.787

100

0.405
  pilQ Legionella pneumophila strain ERS1305867

38.235

98.266

0.376
  pilQ Legionella pneumophila strain Lp02

38.144

98.121

0.374