Detailed information    

experimental Experimentally validated

Overview

Name   cclA/cilC   Type   Machinery gene
Locus tag   SMSK321_RS08155 Genome accession   NZ_AEDT01000025
Coordinates   10266..10925 (+) Length   219 a.a.
NCBI ID   WP_000565601.1    Uniprot ID   -
Organism   Streptococcus mitis SK321     
Function   processing and translocation of ComGC; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 5266..15925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SMSK321_RS08120 (SMSK321_1043) - 5599..7080 (-) 1482 WP_001205288.1 M protein trans-acting positive regulator PRD domain-containing protein -
  SMSK321_RS08125 (SMSK321_1044) - 7366..7602 (+) 237 WP_000964739.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  SMSK321_RS08130 (SMSK321_1045) amaP 7686..8258 (+) 573 WP_000030210.1 alkaline shock response membrane anchor protein AmaP -
  SMSK321_RS08135 (SMSK321_1046) - 8270..8440 (+) 171 WP_000455066.1 DUF2273 domain-containing protein -
  SMSK321_RS08140 (SMSK321_1047) - 8480..9088 (+) 609 WP_000064116.1 Asp23/Gls24 family envelope stress response protein -
  SMSK321_RS08145 (SMSK321_1048) - 9119..9322 (+) 204 WP_000109954.1 CsbD family protein -
  SMSK321_RS12675 - 9500..9724 (-) 225 Protein_11 transposase -
  SMSK321_RS08150 (SMSK321_1049) - 9740..10195 (-) 456 WP_000155439.1 GNAT family N-acetyltransferase -
  SMSK321_RS08155 (SMSK321_1050) cclA/cilC 10266..10925 (+) 660 WP_000565601.1 prepilin peptidase Machinery gene
  SMSK321_RS0102425 trpA 10897..11673 (-) 777 WP_001127016.1 tryptophan synthase subunit alpha -
  SMSK321_RS08160 (SMSK321_1051) trpB 11666..12889 (-) 1224 WP_000331275.1 tryptophan synthase subunit beta -
  SMSK321_RS08165 (SMSK321_1052) - 12867..13466 (-) 600 WP_000169879.1 phosphoribosylanthranilate isomerase -
  SMSK321_RS0102430 trpC 13453..14220 (-) 768 WP_000076525.1 indole-3-glycerol phosphate synthase TrpC -
  SMSK321_RS08170 (SMSK321_1053) trpD 14217..15221 (-) 1005 WP_000658701.1 anthranilate phosphoribosyltransferase -
  SMSK321_RS0102435 - 15232..15798 (-) 567 WP_000601914.1 aminodeoxychorismate/anthranilate synthase component II -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24678.74 Da        Isoelectric Point: 7.6417

>NTDB_id=524 SMSK321_RS08155 WP_000565601.1 10266..10925(+) (cclA/cilC) [Streptococcus mitis SK321]
MIDIYFFLVGSILASFLGLVIDRFPEQSIISPASHCDSCQTRLRPLDLIPILSQVFNHFRCRYCKAPYPVWYALLELGLG
LLFLSYSWGFLSLGQVILITAGLTLGIYDFRHQEYPLLVWMTFHLILMASSGWNLVMVFFLALGILAHFIDIRMGAGDFL
FLASCALVFSATELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLLV

Nucleotide


Download         Length: 660 bp        

>NTDB_id=524 SMSK321_RS08155 WP_000565601.1 10266..10925(+) (cclA/cilC) [Streptococcus mitis SK321]
ATGATTGATATTTATTTTTTTCTTGTCGGGAGCATTCTCGCTTCCTTTCTTGGTTTGGTCATTGACCGTTTTCCAGAGCA
ATCCATTATCAGTCCAGCTAGTCACTGCGATTCCTGTCAGACTCGCTTGCGTCCCTTAGATTTAATTCCAATCCTCTCGC
AGGTTTTCAATCACTTTCGCTGTCGCTACTGCAAGGCTCCTTATCCTGTCTGGTATGCCCTTTTGGAACTAGGCTTAGGT
CTCCTATTTCTATCTTACTCTTGGGGCTTCCTTTCCTTGGGACAAGTCATCCTCATAACTGCTGGCTTGACCTTGGGTAT
CTACGACTTTCGCCATCAGGAATATCCCTTACTGGTCTGGATGACTTTCCACCTAATCCTCATGGCTTCCTCTGGCTGGA
ATCTAGTCATGGTCTTCTTCCTTGCCCTTGGAATTTTAGCTCATTTTATCGATATTCGCATGGGCGCAGGGGATTTTCTC
TTTTTAGCTTCTTGCGCTCTCGTCTTTAGCGCAACAGAATTACTGATCTTGATTCAGTTTGCTTCTGCAACAGGAATTCT
AGCCTTTCTCCTGCAAAAGAAAAAGGAAAGACTTCCTTTCGTGCCTTTCCTCTTACTTGCTACTTGCGTGATTATTTTTG
GTAAGCTACTGCTTGTTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-89)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cclA/cilC Streptococcus pneumoniae Rx1

92.694

100

0.927
  cclA/cilC Streptococcus pneumoniae D39

92.694

100

0.927
  cclA/cilC Streptococcus pneumoniae R6

92.694

100

0.927
  cclA/cilC Streptococcus pneumoniae TIGR4

91.781

100

0.918
  cclA/cilC Streptococcus mitis NCTC 12261

88.128

100

0.881

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]