Detailed information    

experimental Experimentally validated

Overview

Name   cclA/cilC   Type   Machinery gene
Locus tag   SM12261_RS02330 Genome accession   NZ_CP028414
Coordinates   422511..423170 (-) Length   219 a.a.
NCBI ID   WP_000608383.1    Uniprot ID   A0AAX2L6K8
Organism   Streptococcus mitis NCTC 12261     
Function   processing and translocation of ComGC; assembly of the pseudopilus   
DNA binding and uptake

Function

Upregulated in response to CSP as measured by RNA-seq.


Genomic Context

Location: 417511..428170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SM12261_RS02300 (SM12261_0447) - 417632..418198 (+) 567 WP_000601911.1 aminodeoxychorismate/anthranilate synthase component II -
  SM12261_RS02305 (SM12261_0448) trpD 418209..419213 (+) 1005 WP_000658712.1 anthranilate phosphoribosyltransferase -
  SM12261_RS02310 (SM12261_0449) trpC 419210..419983 (+) 774 WP_000076529.1 indole-3-glycerol phosphate synthase TrpC -
  SM12261_RS02315 (SM12261_0450) - 419970..420569 (+) 600 WP_000169924.1 phosphoribosylanthranilate isomerase -
  SM12261_RS02320 (SM12261_0451) trpB 420547..421770 (+) 1224 WP_000331264.1 tryptophan synthase subunit beta -
  SM12261_RS02325 (SM12261_0452) trpA 421763..422539 (+) 777 WP_001127014.1 tryptophan synthase subunit alpha -
  SM12261_RS02330 (SM12261_0453) cclA/cilC 422511..423170 (-) 660 WP_000608383.1 prepilin peptidase Machinery gene
  SM12261_RS02335 (SM12261_0454) - 423240..423695 (+) 456 WP_000155436.1 GNAT family N-acetyltransferase -
  SM12261_RS09750 - 423731..424005 (+) 275 Protein_380 transposase -
  SM12261_RS02345 (SM12261_0455) - 424115..424318 (-) 204 WP_000109960.1 CsbD family protein -
  SM12261_RS02350 (SM12261_0456) - 424349..424957 (-) 609 WP_000064112.1 Asp23/Gls24 family envelope stress response protein -
  SM12261_RS02355 - 424995..425165 (-) 171 WP_000455069.1 DUF2273 domain-containing protein -
  SM12261_RS02360 (SM12261_0457) amaP 425177..425749 (-) 573 WP_000047598.1 alkaline shock response membrane anchor protein AmaP -
  SM12261_RS02365 - 425826..426061 (-) 236 Protein_385 GlsB/YeaQ/YmgE family stress response membrane protein -
  SM12261_RS02370 (SM12261_0458) - 426438..427303 (+) 866 Protein_386 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24965.33 Da        Isoelectric Point: 8.1626

>NTDB_id=496 SM12261_RS02330 WP_000608383.1 422511..423170(-) (cclA/cilC) [Streptococcus mitis NCTC 12261]
MINIYFFLVGSILASFLGLVIDRFPEQSIIQPASHCDSCHTPLRPLDLIPILSQVFNRFRCRYCKVRYPVWYAFFELGLG
LLFLAWSLGWLSLGQVILITAGLTLGIYDFRHQEYPLLVWMTFHIILMAFCGWSLIMLFFLVLGIIAHFIDIRMGAGDFL
FLASCSIVFSVTELLILIQFASAMGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLLV

Nucleotide


Download         Length: 660 bp        

>NTDB_id=496 SM12261_RS02330 WP_000608383.1 422511..423170(-) (cclA/cilC) [Streptococcus mitis NCTC 12261]
ATGATTAATATTTATTTTTTTCTTGTCGGGAGCATTCTCGCTTCCTTTCTTGGTTTGGTTATTGACCGTTTCCCAGAGCA
ATCCATTATCCAACCTGCTAGTCACTGCGATTCCTGTCATACTCCCTTGCGTCCCTTAGATTTGATTCCGATTCTCTCGC
AGGTCTTCAATCGCTTTCGCTGTCGCTACTGCAAGGTTCGCTATCCTGTCTGGTATGCCTTCTTTGAACTAGGCTTAGGA
CTCCTCTTTCTAGCTTGGTCTTTGGGCTGGCTTTCCTTGGGTCAAGTCATCCTGATAACTGCCGGTTTGACCTTGGGCAT
CTACGACTTTCGCCATCAGGAATATCCCTTACTAGTCTGGATGACTTTTCATATAATCCTCATGGCTTTCTGTGGTTGGA
GTCTGATTATGCTCTTCTTTCTTGTTCTAGGAATAATAGCCCATTTTATCGATATCCGTATGGGAGCAGGGGATTTTCTC
TTTTTAGCTTCTTGTTCTATCGTCTTTAGCGTAACGGAGTTACTGATCTTGATTCAATTTGCTTCTGCGATGGGAATTCT
AGCCTTTCTCCTGCAAAAGAAAAAGGAAAGACTTCCTTTCGTACCTTTCCTCTTACTTGCTGCTTGTGTGATTATTTTTG
GTAAGCTACTGCTTGTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-86)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cclA/cilC Streptococcus mitis SK321

88.128

100

0.881
  cclA/cilC Streptococcus pneumoniae Rx1

87.671

100

0.877
  cclA/cilC Streptococcus pneumoniae D39

87.671

100

0.877
  cclA/cilC Streptococcus pneumoniae R6

87.671

100

0.877
  cclA/cilC Streptococcus pneumoniae TIGR4

87.215

100

0.872

Multiple sequence alignment    



References

[1] G Salvadori et al. (2018) High-resolution profiles of the Streptococcus mitis CSP signaling pathway reveal core and strain-specific regulated genes. BMC Genomics 19(1):453. [PMID: 29898666]