Detailed information    

experimental Experimentally validated

Overview

Name   cclA/cilC   Type   Machinery gene
Locus tag   SP_RS08975 Genome accession   NC_003028
Coordinates   1721522..1722181 (+) Length   219 a.a.
NCBI ID   WP_000565004.1    Uniprot ID   A0AA95DDC2
Organism   Streptococcus pneumoniae TIGR4     
Function   processing and translocation of ComGC; assembly of the pseudopilus   
DNA binding and uptake

Function

The late gene cclA is an orthologue of the Bacillus comC gene and type IV prepilin peptidases.


Genomic Context

Location: 1716522..1727181
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SP_RS08935 (SP_1800) mgaSpn 1716678..1718159 (-) 1482 WP_001205280.1 virulence factor transcriptional regulator MgaSpn -
  SP_RS08940 (SP_1801) - 1718602..1718766 (+) 165 WP_001808603.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  SP_RS08945 (SP_1802) amaP 1718850..1719416 (+) 567 WP_000030213.1 alkaline shock response membrane anchor protein AmaP -
  SP_RS08950 (SP_1803) - 1719428..1719598 (+) 171 WP_000455066.1 DUF2273 domain-containing protein -
  SP_RS08955 (SP_1804) - 1719637..1720245 (+) 609 WP_000064115.1 Asp23/Gls24 family envelope stress response protein -
  SP_RS08960 (SP_1805) - 1720276..1720479 (+) 204 WP_000109957.1 CsbD family protein -
  SP_RS08965 (SP_1806) - 1720589..1720861 (-) 273 Protein_1712 transposase -
  SP_RS08970 (SP_1807) - 1721006..1721452 (-) 447 WP_000155427.1 GNAT family N-acetyltransferase -
  SP_RS08975 (SP_1808) cclA/cilC 1721522..1722181 (+) 660 WP_000565004.1 prepilin peptidase Machinery gene
  SP_RS08980 (SP_1809) - 1722233..1723384 (-) 1152 WP_000481750.1 helix-turn-helix domain-containing protein -
  SP_RS08985 (SP_1810) - 1723399..1724130 (-) 732 WP_000934018.1 hypothetical protein -
  SP_RS13065 - 1724356..1724460 (+) 105 Protein_1717 prepilin peptidase -
  SP_RS08990 (SP_1811) trpA 1724432..1725208 (-) 777 WP_001126999.1 tryptophan synthase subunit alpha -
  SP_RS08995 (SP_1812) trpB 1725201..1726424 (-) 1224 WP_000331293.1 tryptophan synthase subunit beta -
  SP_RS09000 (SP_1813) - 1726402..1727001 (-) 600 WP_000169894.1 phosphoribosylanthranilate isomerase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24842.93 Da        Isoelectric Point: 7.6165

>NTDB_id=156 SP_RS08975 WP_000565004.1 1721522..1722181(+) (cclA/cilC) [Streptococcus pneumoniae TIGR4]
MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLRPLDLIPILSQVFNRFRCRYCKVRYPVWYALFELSLG
LLFLLYSWGWLSLGQVVLITAGLTLGIYDFHHQEYPLLVWMTFQLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCLIIFGKLLLV

Nucleotide


Download         Length: 660 bp        

>NTDB_id=156 SP_RS08975 WP_000565004.1 1721522..1722181(+) (cclA/cilC) [Streptococcus pneumoniae TIGR4]
ATGATTGATTTTTATTTTTTTCTCGTCGGGAGCATTCTAGCTTCCTTTCTTGGTTTGGTCATTGACCGTTTTCCAGAGCA
ATCCATTATCAGTTCAGCCAGTCACTGCGATTCCTGTCAGACTCCCTTGCGTCCCTTAGATTTGATTCCGATTCTCTCAC
AGGTCTTCAATCGCTTTCGCTGTCGCTACTGCAAAGTTCGCTATCCTGTCTGGTATGCCCTCTTTGAATTAAGCTTAGGA
CTCCTCTTTCTGCTTTACTCTTGGGGATGGCTCTCCTTGGGGCAAGTCGTCCTAATCACCGCTGGTTTGACCTTGGGTAT
CTACGACTTTCACCATCAGGAATATCCCTTACTGGTCTGGATGACTTTCCAGCTAATCCTAATAGCTTCCTCTGGCTGGA
ATCTGGTCATGGTCTCCTTCCTCATACTTGGAATTTTGGCTCATTTTATCGATATCCGCATGGGTGCAGGGGATTTCCTC
TTTTTAGCTTCTTGTGCTCTCGTCTTTAGCGTAACGGAGTTACTGATCTTGATTCAGTTCGCTTCTGCGACGGGTATCCT
GGCCTTTCTCCTGCAAAAGAAAAAGGAAAGACTTCCTTTCGTGCCTTTCCTCTTACTTGCTACTTGTTTGATTATTTTTG
GTAAGCTACTGCTTGTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-88)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cclA/cilC Streptococcus pneumoniae R6

94.977

100

0.95
  cclA/cilC Streptococcus pneumoniae D39

94.977

100

0.95
  cclA/cilC Streptococcus pneumoniae Rx1

94.977

100

0.95
  cclA/cilC Streptococcus mitis SK321

91.781

100

0.918
  cclA/cilC Streptococcus mitis NCTC 12261

87.215

100

0.872

Multiple sequence alignment    



References

[1] Liming Weng et al. (2013) Exit from competence for genetic transformation in Streptococcus pneumoniae is regulated at multiple levels. PloS One 8(5):e64197. [PMID: 23717566]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]