Detailed information    

experimental Experimentally validated

Overview

Name   cclA/cilC   Type   Machinery gene
Locus tag   KZH43_RS07930 Genome accession   NZ_CP079923
Coordinates   1593089..1593748 (+) Length   219 a.a.
NCBI ID   WP_000565010.1    Uniprot ID   A0A0B7M2C6
Organism   Streptococcus pneumoniae Rx1     
Function   processing and translocation of ComGC; assembly of the pseudopilus   
DNA binding and uptake

Function

The late gene cclA is an orthologue of the Bacillus comC gene and type IV prepilin peptidases.


Genomic Context

Location: 1588089..1598748
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KZH43_RS07890 (KZH43_07890) mgaSpn 1588246..1589727 (-) 1482 WP_108068858.1 virulence factor transcriptional regulator MgaSpn -
  KZH43_RS10865 (KZH43_07895) - 1590111..1590333 (+) 223 Protein_1590 GlsB/YeaQ/YmgE family stress response membrane protein -
  KZH43_RS07900 (KZH43_07900) amaP 1590417..1590983 (+) 567 WP_000030209.1 alkaline shock response membrane anchor protein AmaP -
  KZH43_RS07905 (KZH43_07905) - 1590995..1591165 (+) 171 WP_000455066.1 DUF2273 domain-containing protein -
  KZH43_RS07910 (KZH43_07910) - 1591204..1591812 (+) 609 WP_000064115.1 Asp23/Gls24 family envelope stress response protein -
  KZH43_RS07915 (KZH43_07915) - 1591843..1592046 (+) 204 WP_000109957.1 CsbD family protein -
  KZH43_RS07920 (KZH43_07920) - 1592156..1592395 (-) 240 WP_001005783.1 transposase -
  KZH43_RS07925 (KZH43_07925) - 1592573..1593019 (-) 447 WP_000155429.1 GNAT family N-acetyltransferase -
  KZH43_RS07930 (KZH43_07930) cclA/cilC 1593089..1593748 (+) 660 WP_000565010.1 A24 family peptidase Machinery gene
  KZH43_RS07935 (KZH43_07935) - 1593800..1594951 (-) 1152 WP_000481753.1 ImmA/IrrE family metallo-endopeptidase -
  KZH43_RS07940 (KZH43_07940) - 1594966..1595697 (-) 732 WP_000934017.1 hypothetical protein -
  KZH43_RS07945 (KZH43_07945) - 1595917..1596027 (+) 111 Protein_1600 prepilin peptidase -
  KZH43_RS07950 (KZH43_07950) trpA 1595999..1596775 (-) 777 WP_001127036.1 tryptophan synthase subunit alpha -
  KZH43_RS07955 (KZH43_07955) trpB 1596768..1597991 (-) 1224 WP_000331283.1 tryptophan synthase subunit beta -
  KZH43_RS07960 (KZH43_07960) - 1597969..1598568 (-) 600 WP_000169895.1 phosphoribosylanthranilate isomerase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24845.99 Da        Isoelectric Point: 7.5762

>NTDB_id=266 KZH43_RS07930 WP_000565010.1 1593089..1593748(+) (cclA/cilC) [Streptococcus pneumoniae Rx1]
MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLRPLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLG
LLFLLYSWELLSLSQVILITAGLTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLLV

Nucleotide


Download         Length: 660 bp        

>NTDB_id=266 KZH43_RS07930 WP_000565010.1 1593089..1593748(+) (cclA/cilC) [Streptococcus pneumoniae Rx1]
ATGATTGATTTTTATTTTTTTCTCGTCGGGAGCATTCTAGCTTCCTTTCTTGGTTTGGTCATTGACCGTTTTCCAGAGCA
ATCCATTATCAGTTCAGCCAGTCACTGCGATTCCTGTCAGACTCGCTTGCGTCCCTTAGATTTGATTCCGATTCTCTCAC
AGGTCTTCAATCGCTTTTGCTGTCGCTACTGCAAGGTCCGCTATCCTGTCTGGTATGCCCTCTTTGAACTAGGCTTAGGA
CTCCTCTTTCTGCTTTACTCTTGGGAATTACTTTCCTTGAGTCAAGTCATCCTAATCACTGCTGGTTTGACCTTGGGCAT
CTACGACTTTCGCCATCAGGAATATCCCTTACTGGTCTGGATGACTTTCCACCTAATCCTAATAGCTTCCTCTGGCTGGA
ATCTGGTCATGGTCTCCTTCCTCATACTTGGAATTTTGGCTCATTTTATCGATATCCGCATGGGTGCAGGGGATTTCCTT
TTTTTAGCTTCTTGCGCTCTCGTCTTTAGCGTAACGGAGTTACTGATCTTGATTCAGTTCGCTTCTGCGACGGGTATCCT
GGCCTTTCTCCTGCAAAAGAAAAAGGAAAGACTTCCTTTCGTGCCTTTCCTCTTACTTGCTGCTTGTGTGATTATTTTTG
GTAAGCTACTGCTTGTTTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(8-89)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M2C6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cclA/cilC Streptococcus pneumoniae R6

100

100

1
  cclA/cilC Streptococcus pneumoniae D39

100

100

1
  cclA/cilC Streptococcus pneumoniae TIGR4

94.977

100

0.95
  cclA/cilC Streptococcus mitis SK321

92.694

100

0.927
  cclA/cilC Streptococcus mitis NCTC 12261

87.671

100

0.877

Multiple sequence alignment    



References

[1] Liming Weng et al. (2013) Exit from competence for genetic transformation in Streptococcus pneumoniae is regulated at multiple levels. PloS One 8(5):e64197. [PMID: 23717566]
[2] Scott N Peterson et al. (2004) Identification of competence pheromone responsive genes in Streptococcus pneumoniae by use of DNA microarrays. Molecular Microbiology 51(4):1051-70. [PMID: 14763980]