Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   LL047_RS09990 Genome accession   NZ_CP086072
Coordinates   1980294..1982177 (-) Length   627 a.a.
NCBI ID   WP_347689039.1    Uniprot ID   -
Organism   Lactococcus lactis subsp. lactis strain EIP33     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1975294..1987177
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL047_RS09960 (LL047_09930) macP 1975332..1975610 (-) 279 WP_003130796.1 cell wall synthase accessory phosphoprotein MacP -
  LL047_RS09965 (LL047_09935) - 1975603..1976187 (-) 585 WP_010906182.1 NUDIX hydrolase -
  LL047_RS09970 (LL047_09940) glmU 1976292..1977668 (-) 1377 WP_003130798.1 bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU -
  LL047_RS09975 (LL047_09945) proC 1977896..1978684 (+) 789 WP_010906183.1 pyrroline-5-carboxylate reductase -
  LL047_RS09980 (LL047_09950) - 1978759..1979706 (-) 948 WP_003130410.1 IS30 family transposase -
  LL047_RS09985 (LL047_09955) rpsO 1979843..1980112 (-) 270 WP_010906184.1 30S ribosomal protein S15 -
  LL047_RS09990 (LL047_09960) pknB 1980294..1982177 (-) 1884 WP_347689039.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  LL047_RS09995 (LL047_09965) - 1982177..1982953 (-) 777 WP_003130802.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  LL047_RS10000 (LL047_09970) rsmB 1983034..1984308 (-) 1275 WP_010906185.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LL047_RS10010 (LL047_09980) - 1985430..1986209 (-) 780 WP_163688322.1 peptidoglycan amidohydrolase family protein -
  LL047_RS10015 (LL047_09985) - 1986209..1986496 (-) 288 WP_242166739.1 phage holin -
  LL047_RS10020 (LL047_09990) - 1986509..1986859 (-) 351 WP_014570718.1 hypothetical protein -
  LL047_RS10025 (LL047_09995) - 1986872..1987093 (-) 222 WP_031561031.1 hypothetical protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 67904.00 Da        Isoelectric Point: 4.7783

>NTDB_id=540122 LL047_RS09990 WP_347689039.1 1980294..1982177(-) (pknB) [Lactococcus lactis subsp. lactis strain EIP33]
MIQIGKIFADRYRIIKEIGRGGMANVYQGEDTFLGDRLVAIKVLRSNFENDDIAIARFQREAFAMAELSHPNIVGISDVG
EFESQQYIVMEFVDGMTLKQYINQNAPLANDEAIEIITEILSAMDMAHSHGIIHRDLKPQNVLVSSSGTVKVTDFGIAKA
LSETSLTQTNTMFGSVHYLSPEQARGLNATVQSDIYAIGIILFELLTGQIPFDGDSAVAIALKHFQESIPSIINLNPEVP
QALENVVIKATAKDIKNRYTDVEEMMTDVATSTSLDRRGEEKLVFNKDHDETKIMPANLINPYDTKPLIDKKEDNDSQTD
EKAASSEVGNKNKKSKKGLIIGLIILLLVVGGATLAWVVSTPTNVKIPNVTNSTLSQAKSKIKDAKLKVGTVHKQQSSTI
AEGKVIKTDPTSGTTVRSNSSVDIYVSTGNEDIIKMKDFVGEKIDEAMATLLKDYGIDESQVTQTSVPSDSYPAGTIIKQ
SPKKGSSFDTKGSEKITFEVSSGKQVEVPDYKPNGQYMTYSQYQAALKAAGFTNITLDPQATTNQQADGYVYSVYPNVGT
SVDPTQEIVVTYSVYTAPSSSSTTSESTTTSETSSSTTSSTSSSTTSQPSTDNNNSSKESSTTSSSS

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=540122 LL047_RS09990 WP_347689039.1 1980294..1982177(-) (pknB) [Lactococcus lactis subsp. lactis strain EIP33]
ATGATTCAAATCGGTAAAATCTTTGCCGATCGTTATCGGATTATCAAAGAAATCGGACGTGGCGGAATGGCGAACGTTTA
TCAAGGTGAAGATACTTTTCTTGGTGACCGCTTAGTTGCAATAAAAGTGCTACGTTCAAATTTTGAAAACGATGATATCG
CGATTGCTCGTTTTCAGCGAGAAGCATTCGCCATGGCAGAACTTTCACATCCAAATATCGTAGGTATTTCTGATGTTGGT
GAATTTGAAAGTCAACAATACATTGTCATGGAATTTGTTGACGGCATGACACTTAAACAATACATCAATCAAAATGCACC
TTTAGCCAATGATGAAGCTATTGAAATTATTACTGAAATTTTATCAGCAATGGACATGGCCCATTCACATGGTATTATTC
ACCGGGATTTAAAACCACAAAATGTTTTAGTTTCCAGTAGTGGAACTGTAAAAGTAACGGATTTTGGAATTGCGAAAGCA
CTCTCTGAAACTTCACTTACTCAAACAAATACAATGTTTGGTTCGGTTCATTATTTATCACCAGAGCAAGCGCGTGGTTT
AAATGCAACTGTTCAATCAGATATTTATGCGATTGGAATTATTTTATTTGAGCTTTTAACAGGTCAAATTCCATTTGATG
GAGATTCTGCAGTTGCTATCGCTCTAAAACATTTCCAAGAGAGTATTCCAAGCATCATTAATCTCAATCCAGAAGTTCCG
CAAGCTTTGGAAAATGTTGTCATTAAAGCTACAGCGAAAGATATTAAAAATCGCTATACTGATGTAGAAGAAATGATGAC
TGATGTGGCAACTTCAACGAGTCTTGACCGTCGTGGGGAAGAAAAATTAGTTTTCAATAAGGATCATGATGAAACAAAAA
TTATGCCTGCCAATCTAATTAATCCTTATGACACCAAGCCTCTGATTGATAAAAAAGAAGATAATGATTCACAAACAGAT
GAAAAAGCTGCAAGCTCAGAAGTAGGAAATAAAAACAAAAAATCTAAAAAAGGCTTGATTATTGGTCTTATTATCCTTCT
TTTAGTTGTTGGCGGTGCTACTTTGGCTTGGGTTGTCTCAACACCAACTAATGTTAAGATACCTAATGTGACAAACTCGA
CACTTAGCCAAGCAAAAAGCAAAATTAAAGATGCAAAACTTAAAGTGGGAACAGTTCATAAACAACAAAGTTCAACGATT
GCTGAAGGAAAAGTTATCAAGACTGATCCAACTAGTGGAACAACCGTGAGATCTAATAGCTCCGTTGATATCTATGTTTC
TACGGGAAATGAAGATATCATCAAAATGAAGGACTTTGTCGGTGAAAAGATTGATGAGGCGATGGCAACATTACTTAAGG
ATTATGGTATTGATGAAAGCCAAGTCACTCAAACTTCCGTTCCTAGTGATTCTTACCCAGCAGGTACAATCATTAAACAA
AGCCCTAAAAAAGGTTCTAGTTTTGATACCAAAGGGAGTGAAAAAATTACCTTTGAAGTTTCAAGTGGAAAACAAGTTGA
AGTTCCGGATTATAAACCGAACGGTCAATATATGACTTATTCACAGTATCAAGCTGCCTTAAAAGCTGCAGGATTCACTA
ATATTACACTAGACCCTCAAGCAACAACGAATCAACAAGCTGATGGTTATGTTTACTCAGTCTATCCAAATGTAGGGACA
TCTGTTGATCCAACTCAAGAAATTGTAGTCACTTATAGCGTCTATACTGCTCCAAGTTCAAGTTCAACAACTTCTGAATC
AACTACGACGTCTGAAACTTCATCAAGTACAACTTCAAGTACTTCAAGCTCTACAACAAGTCAGCCGTCAACAGATAACA
ACAATAGCAGCAAAGAATCTTCAACAACGTCATCAAGTAGTTGA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-265)

PASTA domain (PASTA)

(365-428)

PASTA domain (PASTA)

(506-573)

PASTA domain (PASTA)

(434-501)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

49.081

86.762

0.426
  stkP/pknB Streptococcus salivarius strain HSISS4

51.252

82.775

0.424
  stkP Streptococcus pneumoniae TIGR4

47.826

80.702

0.386
  stkP Streptococcus pneumoniae D39

47.628

80.702

0.384
  stkP Streptococcus pneumoniae R6

47.628

80.702

0.384
  stkP Streptococcus pneumoniae Rx1

47.431

80.702

0.383