Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   B5T_RS19730 Genome accession   NC_018691
Coordinates   4403994..4405061 (-) Length   355 a.a.
NCBI ID   WP_148279306.1    Uniprot ID   -
Organism   Alloalcanivorax dieselolei B5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4398994..4410061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5T_RS19705 (B5T_03969) aroK 4399469..4400005 (-) 537 WP_041717145.1 shikimate kinase AroK -
  B5T_RS19710 (B5T_03970) pilQ 4400002..4402152 (-) 2151 WP_014996282.1 type IV pilus secretin PilQ Machinery gene
  B5T_RS19715 (B5T_03971) - 4402173..4402706 (-) 534 WP_014996283.1 pilus assembly protein PilP -
  B5T_RS19720 (B5T_03972) - 4402728..4403426 (-) 699 WP_014996284.1 type 4a pilus biogenesis protein PilO -
  B5T_RS19725 (B5T_03973) - 4403413..4403997 (-) 585 WP_014996285.1 PilN domain-containing protein -
  B5T_RS19730 (B5T_03974) pilM 4403994..4405061 (-) 1068 WP_148279306.1 pilus assembly protein PilM Machinery gene
  B5T_RS19735 (B5T_03975) - 4405396..4407921 (+) 2526 WP_014996287.1 penicillin-binding protein 1A -
  B5T_RS19740 (B5T_03976) - 4407971..4409356 (-) 1386 WP_014996288.1 DKNYY domain-containing protein -

Sequence

Protein


Download         Length: 355 a.a.        Molecular weight: 38371.90 Da        Isoelectric Point: 4.3939

>NTDB_id=53757 B5T_RS19730 WP_148279306.1 4403994..4405061(-) (pilM) [Alloalcanivorax dieselolei B5]
MLPFLRKKAQAMLGVDISSTAVKLLELSRSGGRFKVESYAVEPLTANTVVEKNITDVEGVGDVIARVVARARPGVKAAAV
AVPGSATITKVIEMDASLSEDDMETQLRVEADQYIPFPLDEVRMDFQVLGATEDTPDRVEVLVVASRTENVEMRADALEI
GNLQAKVVDVEAYAMERVFQLVAPALPNADELETVAVFDIGATMTTLNVFHQGRSVYTREQLFGGKQLTEEIQRRYGISM
EEAGLAKKTGELPEDYESEVLQPFKDAIVQQINRSLQFFYGSTQFNQVDYILLAGGSASIAGLPALIQDKVGAGCSLADP
FAGMSKAGKVNVRALANDAPAMMIACGLALRSFEL

Nucleotide


Download         Length: 1068 bp        

>NTDB_id=53757 B5T_RS19730 WP_148279306.1 4403994..4405061(-) (pilM) [Alloalcanivorax dieselolei B5]
GTGCTGCCTTTTCTAAGAAAAAAGGCCCAAGCCATGCTGGGGGTCGATATCAGTTCCACCGCCGTGAAGCTGCTGGAGCT
ATCCAGGTCCGGGGGGCGCTTCAAGGTCGAGAGCTACGCCGTCGAGCCGCTCACGGCCAATACCGTGGTGGAGAAAAACA
TCACCGATGTGGAAGGGGTGGGGGATGTGATCGCCCGGGTGGTTGCCCGGGCCCGCCCGGGGGTCAAGGCCGCCGCCGTG
GCGGTGCCCGGCTCCGCCACCATCACCAAGGTGATCGAAATGGACGCCTCGCTCAGCGAGGACGATATGGAAACGCAATT
GCGGGTGGAGGCGGATCAGTACATCCCGTTCCCCCTGGACGAAGTGCGCATGGATTTTCAGGTGCTCGGCGCCACCGAGG
ATACCCCCGACCGGGTGGAAGTCCTGGTGGTGGCGTCGCGTACGGAGAACGTGGAAATGCGCGCCGATGCCCTGGAAATC
GGTAACCTTCAGGCCAAGGTGGTGGACGTGGAGGCCTACGCCATGGAGCGGGTCTTTCAACTGGTGGCGCCCGCCCTGCC
CAACGCCGATGAGCTGGAAACGGTGGCGGTGTTCGATATCGGCGCCACCATGACCACCCTCAATGTGTTTCATCAAGGGC
GCAGCGTCTATACCCGCGAGCAACTGTTCGGCGGCAAGCAGCTCACCGAGGAAATTCAGCGCCGCTACGGCATCTCCATG
GAGGAGGCCGGGCTGGCGAAGAAAACCGGGGAATTGCCGGAGGACTATGAGAGCGAGGTGTTGCAGCCGTTCAAGGACGC
CATCGTCCAGCAGATCAACCGGTCCCTGCAGTTCTTCTATGGATCCACCCAGTTCAATCAGGTGGATTACATCCTGCTGG
CCGGTGGTTCGGCTTCCATCGCCGGATTGCCGGCGCTGATCCAGGACAAGGTGGGCGCCGGGTGTTCCCTGGCCGATCCC
TTCGCCGGCATGAGCAAGGCCGGCAAGGTCAACGTCCGGGCCCTGGCCAACGACGCCCCGGCGATGATGATCGCCTGCGG
CCTGGCGCTGAGGAGCTTTGAACTATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.367

99.718

0.552
  comM Acinetobacter nosocomialis M2

55.367

99.718

0.552
  comM Acinetobacter baylyi ADP1

51.977

99.718

0.518
  pilM Legionella pneumophila strain ERS1305867

46.893

99.718

0.468

Multiple sequence alignment