Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   JS559_RS02010 Genome accession   NZ_CP069795
Coordinates   431514..431960 (+) Length   148 a.a.
NCBI ID   WP_237611099.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain A-85/14     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 426514..436960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS559_RS01985 (JS559_01990) - 427094..427870 (-) 777 WP_005597402.1 TSUP family transporter -
  JS559_RS01990 (JS559_01995) proB 427883..428974 (-) 1092 WP_005601213.1 glutamate 5-kinase -
  JS559_RS01995 (JS559_02000) folA 429061..429549 (+) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  JS559_RS02000 (JS559_02005) - 429676..429909 (+) 234 WP_005597399.1 YgjV family protein -
  JS559_RS02005 (JS559_02010) radA 429953..431332 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  JS559_RS02010 (JS559_02015) pilA 431514..431960 (+) 447 WP_237611099.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  JS559_RS02015 (JS559_02020) pilB 431978..433372 (+) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  JS559_RS02020 (JS559_02025) pilC 433356..434561 (+) 1206 WP_237611100.1 type II secretion system F family protein Machinery gene
  JS559_RS02025 (JS559_02030) - 434585..435223 (+) 639 WP_005604550.1 prepilin peptidase -
  JS559_RS02030 (JS559_02035) coaE 435245..435883 (+) 639 WP_115586748.1 dephospho-CoA kinase -
  JS559_RS02035 (JS559_02040) yacG 435865..436053 (+) 189 WP_115589824.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 16022.51 Da        Isoelectric Point: 7.6257

>NTDB_id=536657 JS559_RS02010 WP_237611099.1 431514..431960(+) (pilA) [Actinobacillus pleuropneumoniae strain A-85/14]
MQKLSLIRPLTNAFTLIELMIVIAIIAILATVAIPSYNSYTQKAALSELLAASASYKTDVEICIYNTGDSKNCSGGQNGV
RKMTELRQAKYLNAITVEGGTITVTGKGNLQEYGYTMTPIHNGSTISWETKCKGEDLSLFLANFCAIN

Nucleotide


Download         Length: 447 bp        

>NTDB_id=536657 JS559_RS02010 WP_237611099.1 431514..431960(+) (pilA) [Actinobacillus pleuropneumoniae strain A-85/14]
ATGCAAAAACTAAGTCTTATTCGACCGCTTACTAACGCGTTTACTTTAATTGAATTGATGATCGTGATTGCGATTATTGC
CATTTTAGCTACGGTTGCAATTCCGTCATATAACAGTTATACCCAAAAAGCGGCGCTTTCGGAGCTATTGGCGGCATCGG
CTTCTTATAAAACGGATGTCGAGATCTGCATATATAACACCGGAGATTCTAAAAACTGTAGCGGCGGTCAAAACGGTGTC
AGAAAAATGACGGAGCTTAGACAGGCTAAATATTTAAATGCCATTACGGTGGAAGGCGGAACGATTACGGTAACGGGGAA
GGGGAATCTGCAGGAATACGGTTATACGATGACACCGATTCATAACGGTAGCACTATTTCTTGGGAAACGAAATGTAAAG
GGGAGGACTTAAGTTTATTTCTGGCAAATTTCTGTGCGATAAATTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(13-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

68.243

100

0.682
  pilA Haemophilus influenzae 86-028NP

54.483

97.973

0.534
  pilA Haemophilus influenzae Rd KW20

52.414

97.973

0.514