Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   JS559_RS02005 Genome accession   NZ_CP069795
Coordinates   429953..431332 (-) Length   459 a.a.
NCBI ID   WP_005601211.1    Uniprot ID   -
Organism   Actinobacillus pleuropneumoniae strain A-85/14     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 424953..436332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JS559_RS01980 (JS559_01985) nth 426461..427093 (-) 633 WP_005597404.1 endonuclease III -
  JS559_RS01985 (JS559_01990) - 427094..427870 (-) 777 WP_005597402.1 TSUP family transporter -
  JS559_RS01990 (JS559_01995) proB 427883..428974 (-) 1092 WP_005601213.1 glutamate 5-kinase -
  JS559_RS01995 (JS559_02000) folA 429061..429549 (+) 489 WP_005597400.1 type 3 dihydrofolate reductase -
  JS559_RS02000 (JS559_02005) - 429676..429909 (+) 234 WP_005597399.1 YgjV family protein -
  JS559_RS02005 (JS559_02010) radA 429953..431332 (-) 1380 WP_005601211.1 DNA repair protein RadA Machinery gene
  JS559_RS02010 (JS559_02015) pilA 431514..431960 (+) 447 WP_237611099.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  JS559_RS02015 (JS559_02020) pilB 431978..433372 (+) 1395 WP_005601209.1 GspE/PulE family protein Machinery gene
  JS559_RS02020 (JS559_02025) pilC 433356..434561 (+) 1206 WP_237611100.1 type II secretion system F family protein Machinery gene
  JS559_RS02025 (JS559_02030) - 434585..435223 (+) 639 WP_005604550.1 prepilin peptidase -
  JS559_RS02030 (JS559_02035) coaE 435245..435883 (+) 639 WP_115586748.1 dephospho-CoA kinase -
  JS559_RS02035 (JS559_02040) yacG 435865..436053 (+) 189 WP_115589824.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 459 a.a.        Molecular weight: 49678.24 Da        Isoelectric Point: 7.9094

>NTDB_id=536656 JS559_RS02005 WP_005601211.1 429953..431332(-) (radA) [Actinobacillus pleuropneumoniae strain A-85/14]
MAKAPKTAYVCNDCGAEYSRWMGQCKECKSWNTVSEIRLISSKEATKSDRFSGYAGETSGKVQTLSEISLQEVPRFSSGF
NELDRVLGGGVVPGSAILIGGHPGAGKSTLLLQVMCGLSKDLPTLYVTGEESLQQVAMRANRLGLPTDNLKMLSETSVEH
ICNIADQEKPKIMVIDSIQVMHLADIQSSPGSVSQVRECASFLTRYAKTRQVAIIMVGHVTKDGTLAGPKVLEHCIDASI
LLEGESDSRFRTLRSQKNRFGAVNELGVFAMTEQGLREVKNPSAIFLSRSDEQTPGSSVMVLWEGTRPLLVEIQALVDHS
MLANPRRVAVGLDHNRLSLLLAVLHRHGGLQMSDQDVFVNVVGGVKVTETSADLALILALISSFRNRALPQDLVVFGEVG
LAGEIRPVPSGQERISEAAKHGFKRAIIPHGNAPKKPIKGMEVFTVKKLSDALDILGNL

Nucleotide


Download         Length: 1380 bp        

>NTDB_id=536656 JS559_RS02005 WP_005601211.1 429953..431332(-) (radA) [Actinobacillus pleuropneumoniae strain A-85/14]
ATGGCTAAAGCACCAAAAACAGCTTATGTATGTAATGATTGCGGCGCGGAATATTCTCGTTGGATGGGGCAATGCAAAGA
ATGTAAGTCTTGGAATACCGTGAGTGAAATTAGACTTATCTCGAGTAAAGAAGCAACAAAAAGCGACCGCTTCAGCGGAT
ATGCCGGCGAAACCTCCGGTAAAGTTCAAACTTTATCCGAAATCAGCTTACAAGAAGTGCCTCGTTTTAGCAGCGGCTTT
AATGAATTGGATCGCGTACTGGGCGGCGGCGTCGTGCCGGGTTCGGCAATTTTAATCGGCGGCCATCCCGGTGCCGGTAA
ATCCACTCTGCTTTTACAAGTAATGTGCGGTTTGTCTAAAGATTTGCCGACCCTTTACGTGACCGGTGAAGAATCCCTAC
AACAAGTGGCGATGCGAGCGAATCGTTTAGGCTTACCGACCGACAATCTCAAGATGCTTTCAGAAACTTCGGTCGAGCAT
ATTTGTAATATCGCCGACCAAGAAAAGCCGAAAATTATGGTAATCGACTCAATTCAGGTCATGCACCTTGCCGATATTCA
ATCTTCTCCCGGTAGCGTTTCGCAAGTGCGGGAATGCGCTTCGTTTCTGACTCGCTATGCAAAAACCCGCCAAGTAGCGA
TTATTATGGTCGGACACGTCACCAAAGACGGGACACTTGCCGGCCCGAAAGTGTTGGAACACTGTATTGACGCCTCAATT
TTACTGGAAGGCGAATCGGACTCTCGATTCCGTACCTTACGCAGCCAGAAAAACCGTTTCGGTGCGGTCAATGAATTGGG
CGTATTTGCCATGACCGAACAAGGCTTACGAGAAGTAAAAAACCCTTCCGCGATTTTCCTCAGCCGTAGCGATGAACAAA
CGCCGGGCAGTTCCGTGATGGTGCTTTGGGAAGGAACCCGTCCGTTATTAGTCGAAATTCAAGCGTTGGTTGATCATTCG
ATGTTGGCTAATCCTCGCCGTGTCGCTGTAGGTTTAGATCACAACCGTTTATCGCTCTTACTTGCGGTGTTACATCGCCA
CGGCGGTTTGCAAATGTCGGATCAAGATGTGTTCGTCAATGTGGTCGGCGGGGTAAAAGTGACCGAGACCAGTGCCGATC
TCGCCCTAATTTTGGCGTTAATTTCCAGTTTTAGAAATCGTGCGTTACCGCAAGATTTGGTCGTATTCGGCGAGGTGGGA
TTAGCCGGAGAAATTCGTCCGGTACCGAGCGGTCAAGAACGTATCAGCGAAGCGGCGAAGCACGGTTTTAAACGTGCGAT
TATTCCTCACGGCAACGCACCTAAAAAACCGATTAAAGGTATGGAAGTCTTTACCGTTAAGAAACTAAGCGACGCACTAG
ATATTCTCGGCAATCTCTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(83-224)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)

Subunit ChlI of Mg-chelatase (ChlI)

(347-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

48.359

99.564

0.481
  radA Streptococcus mitis NCTC 12261

44.493

98.911

0.44
  radA Streptococcus mitis SK321

44.273

98.911

0.438
  radA Streptococcus pneumoniae Rx1

45.581

93.682

0.427
  radA Streptococcus pneumoniae D39

45.581

93.682

0.427
  radA Streptococcus pneumoniae R6

45.581

93.682

0.427
  radA Streptococcus pneumoniae TIGR4

45.581

93.682

0.427