Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   SCV20265_RS32390 Genome accession   NC_023149
Coordinates   5469755..5470294 (-) Length   179 a.a.
NCBI ID   WP_003116248.1    Uniprot ID   Q59651
Organism   Pseudomonas aeruginosa SCV20265     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5464755..5475294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCV20265_RS25520 (SCV20265_5152) - 5465439..5467694 (-) 2256 WP_023122771.1 DUF1631 domain-containing protein -
  SCV20265_RS25525 (SCV20265_5153) nadC 5467980..5468828 (+) 849 WP_016562338.1 carboxylating nicotinate-nucleotide diphosphorylase -
  SCV20265_RS25535 - 5468993..5469691 (-) 699 WP_023122772.1 hypothetical protein -
  SCV20265_RS32390 pilE 5469755..5470294 (-) 540 WP_003116248.1 pilin Machinery gene
  SCV20265_RS25545 (SCV20265_5154) pilB 5470523..5472226 (+) 1704 WP_003116249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SCV20265_RS25550 (SCV20265_5155) pilC 5472230..5473447 (+) 1218 WP_003116250.1 type II secretion system F family protein Machinery gene
  SCV20265_RS25555 (SCV20265_5156) pilD 5473450..5474322 (+) 873 WP_003116251.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  SCV20265_RS25560 (SCV20265_5157) coaE 5474319..5474930 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  SCV20265_RS25565 (SCV20265_5158) yacG 5474927..5475127 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 179 a.a.        Molecular weight: 18003.27 Da        Isoelectric Point: 4.6894

>NTDB_id=53540 SCV20265_RS32390 WP_003116248.1 5469755..5470294(-) (pilE) [Pseudomonas aeruginosa SCV20265]
MTAQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRARVTEGVGLAASAKTLIGDSSATAGELAASARVWNAQAGNAGAT
SKYVTSVQIAEATGEITVTFNAANVGNIPANSTLVFTPYVQNAAGAPTQLGASYASGVTGSIDWGCASDSNAVSSGTDRN
MPALTAGTLPARFAPSECR

Nucleotide


Download         Length: 540 bp        

>NTDB_id=53540 SCV20265_RS32390 WP_003116248.1 5469755..5470294(-) (pilE) [Pseudomonas aeruginosa SCV20265]
ATGACAGCTCAGAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATTCTTGCTGCCGTCGCTTT
GCCGGCATATCAGGATTACACCATTCGTGCTCGCGTGACAGAGGGGGTTGGCTTGGCTGCCAGCGCCAAGACGCTTATTG
GCGATAGCTCTGCCACTGCCGGTGAGCTAGCCGCTTCGGCAAGGGTCTGGAATGCTCAAGCCGGTAACGCCGGTGCTACC
AGTAAGTATGTGACCTCTGTACAAATTGCAGAGGCGACTGGTGAAATCACTGTTACTTTCAATGCCGCAAACGTGGGTAA
TATTCCGGCTAACTCTACCCTGGTATTTACTCCCTATGTGCAGAATGCTGCCGGTGCCCCGACTCAATTGGGTGCCAGTT
ATGCTTCCGGTGTGACTGGCTCTATTGACTGGGGTTGTGCATCGGACTCCAACGCGGTTTCCAGTGGTACCGACCGCAAT
ATGCCTGCCCTGACTGCAGGTACCCTGCCGGCTCGTTTCGCTCCTAGCGAATGCCGCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(36-179)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q59651

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.451

100

0.447
  pilA Ralstonia pseudosolanacearum GMI1000

44.444

100

0.447
  pilA2 Legionella pneumophila str. Paris

42.373

98.883

0.419
  pilA2 Legionella pneumophila strain ERS1305867

41.808

98.883

0.413
  comP Acinetobacter baylyi ADP1

38.889

100

0.391