Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LFW28_RS18060 Genome accession   NZ_CP084529
Coordinates   3770427..3771551 (+) Length   374 a.a.
NCBI ID   WP_000228551.1    Uniprot ID   P30852
Organism   Escherichia coli strain Origami2-DE3     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3765427..3776551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFW28_RS18035 (LFW28_18035) mscL 3765553..3765963 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  LFW28_RS18040 (LFW28_18040) trkA 3766093..3767469 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  LFW28_RS18045 (LFW28_18045) rsmB 3767491..3768780 (-) 1290 WP_000744778.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LFW28_RS18050 (LFW28_18050) fmt 3768826..3769773 (-) 948 WP_000004473.1 methionyl-tRNA formyltransferase -
  LFW28_RS18055 (LFW28_18055) def 3769788..3770297 (-) 510 WP_000114984.1 peptide deformylase -
  LFW28_RS18060 (LFW28_18060) dprA 3770427..3771551 (+) 1125 WP_000228551.1 DNA-protecting protein DprA Machinery gene
  LFW28_RS18065 (LFW28_18065) smg 3771523..3771996 (+) 474 WP_000460680.1 DUF494 family protein Smg -
  LFW28_RS18070 (LFW28_18070) yrdD 3772025..3772567 (+) 543 WP_001129722.1 type I DNA topoisomerase -
  LFW28_RS18075 (LFW28_18075) tsaC 3772572..3773144 (+) 573 WP_001301412.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  LFW28_RS18080 (LFW28_18080) aroE 3773149..3773967 (+) 819 WP_000451243.1 shikimate dehydrogenase -
  LFW28_RS18085 (LFW28_18085) yrdB 3773964..3774221 (+) 258 WP_001070563.1 DUF1488 domain-containing protein -
  LFW28_RS18090 (LFW28_18090) yrdA 3774197..3774751 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  LFW28_RS18095 (LFW28_18095) - 3775135..3775341 (-) 207 Protein_3566 hypothetical protein -
  LFW28_RS18100 (LFW28_18100) ybfA 3775584..3775790 (-) 207 WP_000424924.1 YbfA family protein -
  LFW28_RS18105 (LFW28_18105) kdpF 3776103..3776192 (+) 90 WP_001272653.1 K(+)-transporting ATPase subunit F -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40954.92 Da        Isoelectric Point: 6.3122

>NTDB_id=535067 LFW28_RS18060 WP_000228551.1 3770427..3771551(+) (dprA) [Escherichia coli strain Origami2-DE3]
MVDTEIWLRLMSISSLYGDDMVRIAHWVAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=535067 LFW28_RS18060 WP_000228551.1 3770427..3771551(+) (dprA) [Escherichia coli strain Origami2-DE3]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG
GGTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P30852

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.238

98.663

0.476
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

45.104

90.107

0.406
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Legionella pneumophila strain ERS1305867

44.242

88.235

0.39