Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   LCF29_RS04190 Genome accession   NZ_CP084096
Coordinates   847444..848937 (+) Length   497 a.a.
NCBI ID   WP_000249344.1    Uniprot ID   -
Organism   Escherichia coli O78 strain 3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 842444..853937
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCF29_RS04170 (LCF29_04170) pilD 843092..843901 (+) 810 WP_000895861.1 prepilin peptidase PppA Machinery gene
  LCF29_RS04175 (LCF29_04175) gspS2 843967..844377 (+) 411 WP_023154867.1 type II secretion system pilot lipoprotein GspS-beta -
  LCF29_RS04180 (LCF29_04180) gspC 844395..845354 (+) 960 WP_000135081.1 type II secretion system protein GspC -
  LCF29_RS04185 (LCF29_04185) gspD 845384..847444 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  LCF29_RS04190 (LCF29_04190) pilB/pilB1 847444..848937 (+) 1494 WP_000249344.1 type II secretion system ATPase GspE Machinery gene
  LCF29_RS04195 (LCF29_04195) gspF 848937..850160 (+) 1224 WP_001173419.1 type II secretion system inner membrane protein GspF -
  LCF29_RS04200 (LCF29_04200) gspG 850177..850632 (+) 456 WP_225343695.1 type II secretion system major pseudopilin GspG -
  LCF29_RS04205 (LCF29_04205) gspH 850636..851199 (+) 564 WP_001115148.1 type II secretion system minor pseudopilin GspH -
  LCF29_RS04210 (LCF29_04210) gspI 851196..851567 (+) 372 WP_000820103.1 type II secretion system minor pseudopilin GspI -
  LCF29_RS04215 (LCF29_04215) gspJ 851564..852169 (+) 606 WP_001250464.1 type II secretion system minor pseudopilin GspJ -
  LCF29_RS04220 (LCF29_04220) gspK 852166..853143 (+) 978 WP_000625913.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55264.47 Da        Isoelectric Point: 6.0390

>NTDB_id=534170 LCF29_RS04190 WP_000249344.1 847444..848937(+) (pilB/pilB1) [Escherichia coli O78 strain 3]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDMVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQPAPA
TDEEKRLLGITDVRTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=534170 LCF29_RS04190 WP_000249344.1 847444..848937(+) (pilB/pilB1) [Escherichia coli O78 strain 3]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACCCTCGCGGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGTGATGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTACGC
CCTGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGTG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAATCAGGCCCGCCTGACGCTGGAGCGTCTGGGGTTGAGTCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTATGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATAGGTCAGACGCAGGTTAACACTCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATATGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCGTCGCTTACCGGGCATCT
GGTGCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTCCGCACGCTGTGCCCCGACTGCCGCCAGCCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGTGCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTCCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTAGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.187

100

0.427
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.765

85.513

0.408
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Acinetobacter baumannii D1279779

46.766

80.885

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Legionella pneumophila strain ERS1305867

43.387

86.72

0.376
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilF Thermus thermophilus HB27

44.75

80.483

0.36