Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   LA372_RS02220 Genome accession   NZ_CP083585
Coordinates   449574..450698 (+) Length   374 a.a.
NCBI ID   WP_000228567.1    Uniprot ID   Q32B64
Organism   Escherichia coli strain ECNB21-M121     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 444574..455698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA372_RS02195 (LA372_02195) mscL 444700..445110 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  LA372_RS02200 (LA372_02200) trkA 445240..446616 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  LA372_RS02205 (LA372_02205) rsmB 446638..447927 (-) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  LA372_RS02210 (LA372_02210) fmt 447973..448920 (-) 948 WP_000004453.1 methionyl-tRNA formyltransferase -
  LA372_RS02215 (LA372_02215) def 448935..449444 (-) 510 WP_000114984.1 peptide deformylase -
  LA372_RS02220 (LA372_02220) dprA 449574..450698 (+) 1125 WP_000228567.1 DNA-protecting protein DprA Machinery gene
  LA372_RS02225 (LA372_02225) smg 450670..451143 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  LA372_RS02230 (LA372_02230) yrdD 451172..451714 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  LA372_RS02235 (LA372_02235) tsaC 451719..452291 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  LA372_RS02240 (LA372_02240) aroE 452296..453114 (+) 819 WP_000451252.1 shikimate dehydrogenase -
  LA372_RS02245 (LA372_02245) yrdB 453111..453368 (+) 258 WP_001070591.1 DUF1488 domain-containing protein -
  LA372_RS02250 (LA372_02250) yrdA 453344..453898 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41022.01 Da        Isoelectric Point: 6.3845

>NTDB_id=532716 LA372_RS02220 WP_000228567.1 449574..450698(+) (dprA) [Escherichia coli strain ECNB21-M121]
MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=532716 LA372_RS02220 WP_000228567.1 449574..450698(+) (dprA) [Escherichia coli strain ECNB21-M121]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCGTCAGCAGCTTGTACGGCGATGATATGGTCCGTATCGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCACGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT
TGAACATGGTGGGGCACTTGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCATTAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q32B64

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Glaesserella parasuis strain SC1401

45.104

90.107

0.406
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

48.71

82.888

0.404
  dprA Vibrio cholerae strain A1552

48.71

82.888

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

43.939

88.235

0.388