Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   K8B73_RS03875 Genome accession   NZ_CP082827
Coordinates   806988..808481 (+) Length   497 a.a.
NCBI ID   WP_000249312.1    Uniprot ID   -
Organism   Escherichia coli strain SCAID WND2-2021 (3/145)     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 801988..813481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K8B73_RS03855 (K8B73_03855) pilD 802636..803445 (+) 810 WP_000895878.1 prepilin peptidase PppA Machinery gene
  K8B73_RS03860 (K8B73_03860) gspS2 803511..803921 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  K8B73_RS03865 (K8B73_03865) gspC 803939..804898 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  K8B73_RS03870 (K8B73_03870) gspD 804928..806988 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  K8B73_RS03875 (K8B73_03875) pilB/pilB1 806988..808481 (+) 1494 WP_000249312.1 type II secretion system ATPase GspE Machinery gene
  K8B73_RS03880 (K8B73_03880) gspF 808481..809704 (+) 1224 WP_001173444.1 type II secretion system inner membrane protein GspF -
  K8B73_RS03885 (K8B73_03885) gspG 809721..810176 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  K8B73_RS03890 (K8B73_03890) gspH 810180..810743 (+) 564 WP_001115118.1 type II secretion system minor pseudopilin GspH -
  K8B73_RS03895 (K8B73_03895) gspI 810740..811111 (+) 372 WP_000820140.1 type II secretion system minor pseudopilin GspI -
  K8B73_RS03900 (K8B73_03900) gspJ 811108..811713 (+) 606 WP_021547736.1 type II secretion system minor pseudopilin GspJ -
  K8B73_RS03905 (K8B73_03905) gspK 811710..812687 (+) 978 WP_223107137.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55164.31 Da        Isoelectric Point: 5.9081

>NTDB_id=530087 K8B73_RS03875 WP_000249312.1 806988..808481(+) (pilB/pilB1) [Escherichia coli strain SCAID WND2-2021 (3/145)]
MVPVAQETIANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYIIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=530087 K8B73_RS03875 WP_000249312.1 806988..808481(+) (pilB/pilB1) [Escherichia coli strain SCAID WND2-2021 (3/145)]
ATGGTGCCTGTAGCACAGGAAACCATCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCAGAAGAACTACCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCAATCATCAAACTGATCAACGCCATGTTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACACTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATTAAGGTGATGGCACGGCTGGATATCGCCGAAAAACGCGTACC
GCAGGATGGGCGCATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATAATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTTGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362