Detailed information NTG0529478

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: JKJ03_RS12205
• Genome accession: NZ_CP068563
• Coordinates: 2523646..2524260 (-)
• Length: 204 a.a.
• NCBI ID: WP_166706122.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain GUMT319
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2518646..2529260

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JKJ03_RS12175 (JKJ03_12175)	rpsT	2518946..2519212 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
JKJ03_RS12180 (JKJ03_12180)	holA	2519229..2520270 (-)	1042	Protein_2366	DNA polymerase III subunit delta	-
JKJ03_RS12185 (JKJ03_12185)	-	2520310..2520464 (-)	155	Protein_2367	hypothetical protein	-
JKJ03_RS12190 (JKJ03_12190)	-	2520505..2520639 (+)	135	WP_003152870.1	YqzM family protein	-
JKJ03_RS12195 (JKJ03_12195)	comEC	2520658..2523009 (-)	2352	WP_202735636.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
JKJ03_RS12200 (JKJ03_12200)	-	2523010..2523579 (-)	570	WP_003152868.1	ComE operon protein 2	-
JKJ03_RS12205 (JKJ03_12205)	comEA	2523646..2524260 (-)	615	WP_166706122.1	helix-hairpin-helix domain-containing protein	Machinery gene
JKJ03_RS12210 (JKJ03_12210)	comER	2524319..2525140 (+)	822	WP_015240246.1	late competence protein ComER	-
JKJ03_RS12215 (JKJ03_12215)	-	2525209..2525946 (-)	738	WP_166706119.1	class I SAM-dependent DNA methyltransferase	-
JKJ03_RS12220 (JKJ03_12220)	rsfS	2525943..2526299 (-)	357	WP_003152864.1	ribosome silencing factor	-
JKJ03_RS12225 (JKJ03_12225)	yqeK	2526317..2526877 (-)	561	WP_032856887.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
JKJ03_RS12230 (JKJ03_12230)	-	2526867..2527436 (-)	570	WP_003152860.1	nicotinate-nucleotide adenylyltransferase	-
JKJ03_RS12235 (JKJ03_12235)	yhbY	2527447..2527737 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
JKJ03_RS12240 (JKJ03_12240)	aroE	2527731..2528573 (-)	843	WP_015417858.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22220.30 Da
• Isoelectric Point: 5.1985

>NTDB_id=529478 JKJ03_RS12205 WP_166706122.1 2523646..2524260(-) (comEA) [Bacillus velezensis strain GUMT319]
MTKAMMTERLRWNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=529478 JKJ03_RS12205 WP_166706122.1 2523646..2524260(-) (comEA) [Bacillus velezensis strain GUMT319]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGTGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCCT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Lactococcus lactis subsp. cremoris KW2	33.778	100	0.373
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.058	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.577	100	0.363
comEA	Staphylococcus aureus MW2	34.579	100	0.363