NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	K8B89_RS22425	Genome accession	NZ_CP082775
Coordinates	4531524..4532648 (-)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	A0A0H3EME5
Organism	Escherichia coli O25:H4 strain CU568-3
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 4526524..4537648

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K8B89_RS22395 (K8B89_22235)	yrdA	4528324..4528878 (+)	555	WP_000005712.1	gamma carbonic anhydrase family protein	-
K8B89_RS22400 (K8B89_22240)	yrdB	4528854..4529111 (-)	258	WP_001070565.1	DUF1488 domain-containing protein	-
K8B89_RS22405 (K8B89_22245)	aroE	4529108..4529926 (-)	819	WP_000451255.1	shikimate dehydrogenase	-
K8B89_RS22410 (K8B89_22250)	tsaC	4529931..4530503 (-)	573	WP_001296460.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
K8B89_RS22415 (K8B89_22255)	yrdD	4530508..4531050 (-)	543	WP_001296461.1	type I DNA topoisomerase	-
K8B89_RS22420 (K8B89_22260)	smg	4531079..4531552 (-)	474	WP_000460673.1	DUF494 family protein Smg	-
K8B89_RS22425 (K8B89_22265)	dprA	4531524..4532648 (-)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
K8B89_RS22430 (K8B89_22270)	def	4532778..4533287 (+)	510	WP_000114984.1	peptide deformylase	-
K8B89_RS22435 (K8B89_22275)	fmt	4533302..4534249 (+)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
K8B89_RS22440 (K8B89_22280)	rsmB	4534301..4535590 (+)	1290	WP_000744765.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
K8B89_RS22445 (K8B89_22285)	trkA	4535612..4536988 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
K8B89_RS22450 (K8B89_22290)	mscL	4537118..4537531 (+)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=528897 K8B89_RS22425 WP_000228517.1 4531524..4532648(-) (dprA) [Escherichia coli O25:H4 strain CU568-3]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=528897 K8B89_RS22425 WP_000228517.1 4531524..4532648(-) (dprA) [Escherichia coli O25:H4 strain CU568-3]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H3EME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0528897